Code | CSB-RA194949A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
IP | 1:200-1:1000 |
The KLF4 recombinant monoclonal antibody is produced using recombinant DNA technology and is suitable for detecting human KLF4 protein in ELISA, WB, IHC, IF, and IP applications. To produce the antibody, the gene coding for the KLF4 monoclonal antibody is synthesized by sequencing the cDNA of the KLF4 antibody-producing hybridomas. The hybridomas are generated by fusing myeloma cells with B cells isolated from an animal that was immunized with a synthesized peptide derived from human KLF4. The synthesized gene is then cloned into a vector and transfected into cells for cultivation. After culturing, the resulting KLF4 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography.
The KLF4 protein is a transcription factor that can bind to DNA and regulate the expression of target genes. It plays important roles in various cellular processes, including cell proliferation, differentiation, and apoptosis. KLF4 has also been shown to function as a tumor suppressor in some contexts by inducing cell cycle arrest and promoting apoptosis. It has also been implicated in promoting cell differentiation in a variety of cell types, including skin cells, gut epithelial cells, and immune cells.
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