Human L-lactate dehydrogenase B chain(LDHB) ELISA kit

Instructions
Code CSB-EL012841HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lactate dehydrogenase B
Alternative Names Epididymis secretory protein Li 281 ELISA Kit; HEL S 281 ELISA Kit; L lactate dehydrogenase B chain ELISA Kit; L-lactate dehydrogenase B chain ELISA Kit; Lactate Dehydrogenase B ELISA Kit; Lactate dehydrogenase H chain ELISA Kit; LDH B ELISA Kit; LDH H ELISA Kit; LDH heart subunit ELISA Kit; LDH-B ELISA Kit; LDH-H ELISA Kit; LDHB ELISA Kit; LDHB_HUMAN ELISA Kit; LDHBD ELISA Kit; LDHH ELISA Kit; Renal carcinoma antigen NY REN 46 ELISA Kit; Renal carcinoma antigen NY-REN-46 ELISA Kit; TRG-5 ELISA Kit; TRG5 ELISA Kit
Abbreviation LDHB
Uniprot No. P07195
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cerebrospinal fluid (CSF), urine.
Detection Range 23.5 mU/mL-1500 mU/mL
Sensitivity 5.8 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LDHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 89    
Range % 84-93    
1:2 Average % 100    
Range % 97-103    
1:4 Average % 87    
Range % 84-90    
1:8 Average % 102    
Range % 98-106    
Recovery
The recovery of human LDHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 103 100-106    
EDTA plasma (n=4) 89 85-92    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
mU/ml OD1 OD2 Average Corrected    
1500 2.184 2.203 2.194 2.038    
750 1.706 1.697 1.702 1.546    
375 1.215 1.241 1.228 1.072    
187.5 0.757 0.738 0.748 0.592    
94 0.442 0.468 0.455 0.299    
47 0.319 0.298 0.309 0.153    
23.5 0.244 0.231 0.238 0.082    
0 0.159 0.153 0.156      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Gene References into Functions
  1. High LDHB expression is significantly associated with the level of serum LDH and better clinical outcomes in lung squamous cell carcinoma PMID: 28756978
  2. this study shows that low base line lactate dehydrogenase is linked with a better tumor response, and that prolonged overall survival and progression-free survival are observed for patients under ipilimumab treatment with no increase in lactate dehydrogenase PMID: 27485076
  3. our study examines the inclusion of salivary LDH as potential diagnostic parameter and therapeutic index in Oral squamous cell carcinoma PMID: 26577856
  4. addition of exogenous lactic acid to growth media was sufficient to induce cell death that could be inhibited by the expression of LDHB. the results suggest that lactate dehydrogenase is a general suppressor of programmed cell death in yeast. PMID: 26032856
  5. The aim of this study was to demonstrate the effects of 6-week low-intensity training on changes in indicators of aerobic capacity and on HSPA1A, HSPB1, and LDHb expression in white blood cells in high level rowers. PMID: 26214432
  6. Lactate dehydrogenase B is associated with the response to neoadjuvant chemotherapy in oral squamous cell carcinoma. PMID: 25973606
  7. The readthrough-extended lactate dehydrogenase subunit LDHBx can also co-import LDHA, the other LDH subunit, into peroxisomes. PMID: 25247702
  8. We were able to provide evidence that methylation of HLTF and especially HPP1 detected in serum is strongly correlated with cell death in CRC using LDH as surrogate marker PMID: 24708595
  9. This meta-analysis shows that high serum LDH level is obviously associated with lower overall survival rate in patients with osteosarcoma, and it is an effective biomarker of prognosis. PMID: 24682390
  10. These observations support prospective clinical evaluation of LDHB as a predictive marker of response for patients with breast cancer receiving neoadjuvant chemotherapy. PMID: 23697991
  11. This study identifies LDHB as a regulator of cell proliferation in a subset of lung adenocarcinoma and may provide a novel therapeutic approach for treating lung cancer PMID: 23224736
  12. LDH concentrations in nasopharyngeal secretions are positively associated with acute otitis media risk. PMID: 23202721
  13. Loss of LDH-B expression is an early and frequent event in human breast cancer occurring due to promoter methylation, and is likely to contribute to an enhanced glycolysis of cancer cells under hypoxia. PMID: 23437403
  14. ata indicate that serum lactic dehydrogenase (S-LDH) appears to be a significant independent prognostic index in patients with metastatic nasopharyngeal carcinoma (NPC). PMID: 23266049
  15. Patients with severe vaso-occlusive crisis were more frequently males, who also had higher white blood cell (WBC) count, procalcitonin (PCT), and lactate dehydrogenase (LDH) levels. PMID: 22892192
  16. Data reveal that LDHB is upregulated and required only in certain cancer genotypes. PMID: 23139210
  17. results indicate that histone deacetylase inhibitors upregulate miRNAs, at least some of which act as tumor suppressors. lactate dehydrogenase B, which is regulated by the tumor suppressive miR-375, may therefore act as an oncogene in esophageal squamous cell carcinoma. PMID: 22752059
  18. Serum lactate dehydrogenase is a prognostic and predictive biomarker for survival benefit conferred by TORC1 inhibition in poor-risk renal cell carcinoma. PMID: 22891270
  19. Data indicate that serum lactate dehydrogenase (LDH) seemed able to predict clinical outcome for hepatocellular carcinoma (HCC) patients undergoing hepatocellular carcinoma (HCC). PMID: 22461886
  20. The significant elevation in serum CK [creatine kinase ]and LDH [L-Lactate Dehydrogenase ] activities indicates that these can be used as parameters for screening hypothyroid patients but not hyperthyroid patients. PMID: 22248949
  21. results confirmed the prognostic roles of LDH-B in urinary bladder urothelial carcinoma. PMID: 22027740
  22. Results suggest that lactate dehydrogenase B suppression plays an important role in triggering or maintaining the mitochondrial defects and then contributes to cancer cell invasiveness by inducing claudin-1 protein. PMID: 21356207
  23. Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. PMID: 20499337
  24. findings offer proof of concept for targeting LDHB as a therapeutic strategy in cancers driven by aberrant activation of the RTK-PI3K-AKT-mTOR signaling cascade PMID: 21199794
  25. S100B and LDH are not expressed in sentinel node progression of melanoma PMID: 20592382
  26. LDHB was elevated in idiopathic myelofibrosis. This isoenzymatic pattern could be expression of a metabolic adaptation. PMID: 17178662
  27. MYC, LDHB, and CCNB1 may have roles in progression of medulloblastoma PMID: 18593994

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Involvement in disease Lactate dehydrogenase B deficiency (LDHBD)
Subcellular Location Cytoplasm
Protein Families LDH/MDH superfamily, LDH family
Database Links

HGNC: 6541

OMIM: 150100

KEGG: hsa:3945

STRING: 9606.ENSP00000229319

UniGene: Hs.446149

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