Human Malate Dehydrogenase 1 (MDH1) ELISA Kit

Code CSB-E13662h
Size 96T,5×96T,10×96T
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Product Details

Target Name
malate dehydrogenase 1, NAD (soluble)
Alternative Names
cytoplasmic ELISA Kit; Cytosolic malate dehydrogenase ELISA Kit; Diiodophenylpyruvate reductase ELISA Kit; Malate dehydrogenase 1, NAD (soluble) ELISA Kit; Malate dehydrogenase ELISA Kit; Malate dehydrogenase cytoplasmic ELISA Kit; MDH s ELISA Kit; mdh1 ELISA Kit; MDHA ELISA Kit; MDHC_HUMAN ELISA Kit; MDHs ELISA Kit; MGC:1375 ELISA Kit; MOR2 ELISA Kit; Soluble malate dehydrogenase ELISA Kit
Abbreviation
MDH1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
9.38 mU/mL-600 mU/mL
Sensitivity
2.35 mU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MDH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %91-99
1:2Average %102
Range %97-107
1:4Average %110
Range %107-113
1:8Average %86
Range %83-89
Recovery
The recovery of human MDH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9188-94
EDTA plasma (n=4)9491-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
6002.157 2.198 2.178 2.053
3001.766 1.738 1.752 1.627
1501.255 1.269 1.262 1.137
750.764 0.798 0.781 0.656
37.50.512 0.531 0.522 0.397
18.750.305 0.324 0.315 0.190
9.380.198 0.188 0.193 0.068
00.126 0.124 0.125
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human MDH1 ELISA Kit was designed for the quantitative measurement of Human MDH1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 9.38 mU/mL-600 mU/mL and the sensitivity is 2.35 mU/mL.

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Target Background

Function
(From Uniprot)
Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation.
Gene References into Functions
  1. expression of miR-126-5p suppresses the enzymatic activity of MDH1, mitochondrial respiration and caused cell death in non-small cell lung carcinoma cell lines. PMID: 29574159
  2. Data show that in the endogenous readthrough of the human MDH1 stop codon, the stop codon can encode tryptophan and arginine, and is tissue-specific. PMID: 27881739
  3. Proliferating cells rely on both MDH1 and LDH to replenish cytosolic NAD. PMID: 28263970
  4. Arginine methylation of MDH1 by CARM1 regulates cellular redox homeostasis and suppresses glutamine metabolism of pancreatic cancer. PMID: 27840030
  5. adipogenic differentiation may be regulated by the acetylation of MDH1 PMID: 22693256
  6. The MDH1 gene is not the cause of RP28-linked autosomal recessive retinitis pigmentosa. PMID: 20011630
  7. expression of MDH1 is maintained in the adult heart but is not present in levels as high as in the fetus PMID: 15565635
  8. Malate Dehydrogenase directly regulates the Tumor Suppressor Protein p53-dependent apoptosis upon glucose deprivation and involved in maintaining cellular metabolic state and further determining cell death. PMID: 19229245

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Subcellular Location
Cytoplasm.
Protein Families
LDH/MDH superfamily, MDH type 2 family
Database Links

HGNC: 6970

OMIM: 154200

KEGG: hsa:4190

STRING: 9606.ENSP00000438144

UniGene: Hs.526521

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