MDH1 Antibody

Code CSB-PA013621EA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: HL60 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, LO2 whole cell lysate, NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse liver tissue
    All lanes: MDH1 antibody at 2.6µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 37, 28, 39 kDa
    Observed band size: 37 kDa

  • IHC image of CSB-PA013621EA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of U251 cells with CSB-PA013621EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) MDH1 Polyclonal antibody
Uniprot No.
Target Names
MDH1
Alternative Names
cytoplasmic antibody; Cytosolic malate dehydrogenase antibody; Diiodophenylpyruvate reductase antibody; Malate dehydrogenase 1, NAD (soluble) antibody; Malate dehydrogenase antibody; Malate dehydrogenase cytoplasmic antibody; MDH s antibody; mdh1 antibody; MDHA antibody; MDHC_HUMAN antibody; MDHs antibody; MGC:1375 antibody; MOR2 antibody; Soluble malate dehydrogenase antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Malate dehydrogenase, cytoplasmic protein (1-334AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The MDH1 Antibody (Product code: CSB-PA013621EA01HU) is Non-conjugated. For MDH1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA013621EB01HU MDH1 Antibody, HRP conjugated ELISA
FITC CSB-PA013621EC01HU MDH1 Antibody, FITC conjugated
Biotin CSB-PA013621ED01HU MDH1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation.
Gene References into Functions
  1. expression of miR-126-5p suppresses the enzymatic activity of MDH1, mitochondrial respiration and caused cell death in non-small cell lung carcinoma cell lines. PMID: 29574159
  2. Data show that in the endogenous readthrough of the human MDH1 stop codon, the stop codon can encode tryptophan and arginine, and is tissue-specific. PMID: 27881739
  3. Proliferating cells rely on both MDH1 and LDH to replenish cytosolic NAD. PMID: 28263970
  4. Arginine methylation of MDH1 by CARM1 regulates cellular redox homeostasis and suppresses glutamine metabolism of pancreatic cancer. PMID: 27840030
  5. adipogenic differentiation may be regulated by the acetylation of MDH1 PMID: 22693256
  6. The MDH1 gene is not the cause of RP28-linked autosomal recessive retinitis pigmentosa. PMID: 20011630
  7. expression of MDH1 is maintained in the adult heart but is not present in levels as high as in the fetus PMID: 15565635
  8. Malate Dehydrogenase directly regulates the Tumor Suppressor Protein p53-dependent apoptosis upon glucose deprivation and involved in maintaining cellular metabolic state and further determining cell death. PMID: 19229245

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Subcellular Location
Cytoplasm.
Protein Families
LDH/MDH superfamily, MDH type 2 family
Database Links

HGNC: 6970

OMIM: 154200

KEGG: hsa:4190

STRING: 9606.ENSP00000438144

UniGene: Hs.526521

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