MDH1 Antibody

Datasheet
Code CSB-PA013621EA01HU
See More Details
Size US$299
Uniprot No. P40925
Image
  • Western Blot
    Positive WB detected in: HL60 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, LO2 whole cell lysate, NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse liver tissue
    All lanes: MDH1 antibody at 2.6µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 37, 28, 39 kDa
    Observed band size: 37 kDa

  • IHC image of CSB-PA013621EA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of U251 cells with CSB-PA013621EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunogen Recombinant Human Malate dehydrogenase, cytoplasmic protein (1-334AA)
Raised in Rabbit
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Relevance cytosol, extracellular exosome, extracellular space, mitochondrion, L-malate dehydrogenase activity, malic enzyme activity, gluconeogenesis, malate metabolic process, NADH metabolic process, oxaloacetate metabolic process
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Malate dehydrogenase, cytoplasmic (EC 1.1.1.37) (Cytosolic malate dehydrogenase) (Diiodophenylpyruvate reductase) (EC 1.1.1.96), MDH1, MDHA
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Target Names MDH1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Subcellular Location Cytoplasm
Protein Families LDH/MDH superfamily, MDH type 2 family
Database Links

HGNC: 6970

OMIM: 154200

KEGG: hsa:4190

STRING: 9606.ENSP00000438144

UniGene: Hs.526521

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