Human Pepsin,PG ELISA Kit

Code CSB-E08919h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

This Human Pepsin (PG) ELISA Kit was designed for the quantitative measurement of Human Pepsin (PG) protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .

Target Name Pepsin,PG
Alternative Names PGA3 ELISA Kit; Pepsin A-3 ELISA Kit; EC 3.4.23.1 ELISA Kit; Pepsinogen-3 ELISA Kit
Abbreviation PG
Uniprot No. P0DJD8
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %90
Range %84-95
1:200Average %93
Range %88-99
1:400Average %99
Range %95-104
1:800Average %101
Range %96-108
Recovery
The recovery of human PG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9893-104
EDTA plasma (n=4)9489-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.559 2.598 2.579 2.401
10002.062 2.079 2.071 1.893
5001.439 1.415 1.427 1.249
2500.867 0.855 0.861 0.683
1250.549 0.538 0.544 0.366
62.50.426 0.463 0.445 0.267
31.250.354 0.364 0.359 0.181
00.177 0.179 0.178  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Gene References into Functions
  1. Low Pepsinogen I is associated with precancerous gastric lesions. PMID: 25292040
  2. High serum Pepsinogen I level is associ9ated with the progression of gastric precancerous lesions. PMID: 24895149
  3. Significantly higher AUC was observed for the PGI/PGII ratio and is associated with atrophy and intestinal metaplasia. PMID: 23886209
  4. A significant negative correlation was found between the degree of corpus atrophy and the level of serum pepsinogen-I in atrophic gastritis patients with gastroesophageal reflux. PMID: 21175799
  5. Higher levels of Pepsinogen I is associated with early gastric cancer PMID: 15688378
  6. Helicobacter pylori eradication improves gastric histology and decreases serum gastrin, pepsinogen I and pepsinogen II levels in patients with duodenal ulcer. PMID: 17559360
  7. pepsinogens may have a role in esophageal squamous dysplasia PMID: 18844222
  8. Barrett's esophagus is characterized by the absence of Helicobacter pylori infection and high levels of serum pepsinogen I concentration in Japan. PMID: 19196398

Show More

Hide All

Subcellular Location Secreted.
Protein Families Peptidase A1 family
Database Links

HGNC: 8885

OMIM: 169700

KEGG: hsa:643834

STRING: 9606.ENSP00000322192

UniGene: Hs.601055

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2022 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1