Human Protein FAM132A(FAM132A) ELISA kit

Instructions
Code CSB-EL008058HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name family with sequence similarity 132, member A
Alternative Names C1QTNF12 ELISA Kit; C1QDC2 ELISA Kit; CTRP12 ELISA Kit; FAM132AAdipolin ELISA Kit; Adipose-derived insulin-sensitizing factor ELISA Kit; C1q and TNF related protein 12 ELISA Kit; Complement C1q tumor necrosis factor-related protein 12) [Cleaved into: Adipolin fC1QTNF12 ELISA Kit; Adipolin fCTRP12 ELISA Kit; Adipolin full-length form); Adipolin gC1QTNF12 ELISA Kit; Adipolin cleaved form ELISA Kit; Adipolin gCTRP12)] ELISA Kit
Abbreviation FAM132A
Uniprot No. Q5T7M4
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FAM132A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %85-89
1:2Average %99
Range %91-102
1:4Average %100
Range %93-108
1:8Average %94
Range %90-99
Recovery
The recovery of human FAM132A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-96
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.380 2.356 2.368 2.237
10001.815 1.776 1.796 1.665
5001.220 1.198 1.209 1.078
2500.669 0.666 0.668 0.537
1250.438 0.424 0.431 0.300
62.50.226 0.222 0.224 0.093
31.250.181 0.178 0.180 0.049
00.134 0.128 0.131  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Insulin-sensitizing adipocyte-secreted protein (adipokine) that regulates glucose metabolism in liver and adipose tissue. Promotes glucose uptake in adipocytes and suppresses de novo glucose production in hepatocytes via the PI3K-Akt signaling pathway. Administration lead to reduction of blood glucose. Able to attenuate inflammation in fat tissue.
Gene References into Functions
  1. Adipolin and adiponectin are cytokines that exert substantial impact on obesity, progression of atherosclerosis, insulin resistance, and glucose metabolism. PMID: 29452073
  2. Low serum CTRP12 level is associated with Type 2 diabetes mellitus. PMID: 28207876
  3. Coenzyme Q10 reduced HbA1c considerably in overweight and obese patients with diabetes, although interestingly adipolin levels declined simultaneously. In this study, Q10 modulated glucose homeostasis, which was expected to be mediated by increasing adipolin. The similar mechanisms of action of Q10 and adipolin may justify lowering effect of Q10 on adipolin. PMID: 27657997
  4. Serum adipolin concentrations were significantly lower in polycystic ovarian syndrome compared with control subjects. PMID: 24612181
  5. In subcutaneous adipose tissue explants, insulin significantly increased CTRP12 protein expression and secretion. PMID: 24492466
  6. we describe, for the first time, adipolin expression in human chondrocytes as well as the intracellular mechanism involved in its regulation PMID: 22736092
  7. Endopeptidase cleavage generates a functionally distinct isoform of C1q/tumor necrosis factor-related protein-12 (CTRP12) with an altered oligomeric state and signaling specificity. PMID: 22942287
  8. Reports cloning, expression, regulation and function of mouse adipolin (Fam132a) PMID: 21849507

Show More

Hide All

Subcellular Location Adipolin fC1QTNF12: Secreted, SUBCELLULAR LOCATION: Adipolin gC1QTNF12: Secreted
Protein Families Adipolin/erythroferrone family
Tissue Specificity Predominantly expressed by adipose tissues.
Database Links

HGNC: 32308

OMIM: 616593

KEGG: hsa:388581

STRING: 9606.ENSP00000329137

UniGene: Hs.197613

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1