Human Protein FAM132B(FAM132B) ELISA kit

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Name: Human Protein FAM132B(FAM132B) ELISA kit
Brand: CUSABIO
SKU: CSB-EL008059HU

Product Details

Description

This Human FAM132B ELISA Kit was designed for the quantitative measurement of Human FAM132B protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

Target Name

family with sequence similarity 132, member B

Alternative Names

Complement C1q tumor necrosis factor-related protein 15 ELISA Kit; ERFE_HUMAN ELISA Kit; Erythroferrone ELISA Kit; FAM132B ELISA Kit; Myonectin ELISA Kit

Abbreviation

FAM132B

Uniprot No.

Q4G0M1

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates, cell lysates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FAM132B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-95
1:2	Average %	95
	Range %	90-101
1:4	Average %	87
	Range %	83-94
1:8	Average %	99
	Range %	94-105

Recovery

The recovery of human FAM132B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-102
EDTA plasma (n=4)	88	84-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.455	2.430	2.443	2.344
500	2.201	2.250	2.226	2.127
250	1.705	1.854	1.780	1.681
125	1.110	1.202	1.156	1.057
62.5	0.699	0.741	0.720	0.621
31.2	0.435	0.422	0.429	0.330
15.6	0.266	0.270	0.268	0.169
0	0.103	0.095	0.099

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Customer Reviews and Q&A

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■ Q&A

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.

Thanks for your inquiry!
CSB-EL008059HU
This kit is designed against full length. Pls refer to:https://www.uniprot.org/uniprot/Q4G0M1.
Capture antibody is rabbit polyclonal antibody and detection antibody is rabbit polyclonal antibody.
Standard is 293 cell-derived recombinant protein.
Immunogen is 293 cell-derived recombinant protein.

Target Background

Function (From Uniprot)	Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.
Gene References into Functions	From a clinical point of view, erythroferrone could become a useful biological marker of iron metabolism and a therapeutic target. [review] PMID: 28666715 FAM132b expression is upregulated in Congenital Dyserythropoietic Anemia type II. PMID: 27540014 In hemodialysis patients DA and CERA increased levels of ERFE that regulated hepcidin 25 and led to iron mobilization from body stores during erythropoiesis. PMID: 26978524 Myonectin links skeletal muscle to lipid homeostasis in liver and adipose tissue in response to alterations in energy state, revealing a novel myonectin-mediated metabolic circuit. PMID: 22351773
Subcellular Location	Secreted.
Protein Families	Adipolin/erythroferrone family
Database Links	HGNC: 26727 OMIM: 615099 KEGG: hsa:151176 UniGene: Hs.24951

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Check Code：

Code	CSB-EL008059HU
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.