Human Protein FAM132B(FAM132B) ELISA kit

Code CSB-EL008059HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
family with sequence similarity 132, member B
Alternative Names
Complement C1q tumor necrosis factor-related protein 15 ELISA Kit; ERFE_HUMAN ELISA Kit; Erythroferrone ELISA Kit; FAM132B ELISA Kit; Myonectin ELISA Kit
Abbreviation
FAM132B
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FAM132B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 86-95  
1:2 Average % 95  
Range % 90-101  
1:4 Average % 87  
Range % 83-94  
1:8 Average % 99  
Range % 94-105  
Recovery
The recovery of human FAM132B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-102  
EDTA plasma (n=4) 88 84-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.455 2.430 2.443 2.344  
500 2.201 2.250 2.226 2.127  
250 1.705 1.854 1.780 1.681  
125 1.110 1.202 1.156 1.057  
62.5 0.699 0.741 0.720 0.621  
31.2 0.435 0.422 0.429 0.330  
15.6 0.266 0.270 0.268 0.169  
0 0.103 0.095 0.099    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human FAM132B ELISA Kit was designed for the quantitative measurement of Human FAM132B protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

Customer Reviews and Q&A

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 Q&A
Q:

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.

A:
Thanks for your inquiry!
CSB-EL008059HU
This kit is designed against full length. Pls refer to:https://www.uniprot.org/uniprot/Q4G0M1.
Capture antibody is rabbit polyclonal antibody and detection antibody is rabbit polyclonal antibody.
Standard is 293 cell-derived recombinant protein.
Immunogen is 293 cell-derived recombinant protein.

Target Background

Function
(From Uniprot)
Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.
Gene References into Functions
  1. From a clinical point of view, erythroferrone could become a useful biological marker of iron metabolism and a therapeutic target. [review] PMID: 28666715
  2. FAM132b expression is upregulated in Congenital Dyserythropoietic Anemia type II. PMID: 27540014
  3. In hemodialysis patients DA and CERA increased levels of ERFE that regulated hepcidin 25 and led to iron mobilization from body stores during erythropoiesis. PMID: 26978524
  4. Myonectin links skeletal muscle to lipid homeostasis in liver and adipose tissue in response to alterations in energy state, revealing a novel myonectin-mediated metabolic circuit. PMID: 22351773
Subcellular Location
Secreted.
Protein Families
Adipolin/erythroferrone family
Database Links

HGNC: 26727

OMIM: 615099

KEGG: hsa:151176

UniGene: Hs.24951

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