Recombinant Human Erythroferrone (ERFE), partial

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Code CSB-EP677939HU1
Size US$388
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  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP677939HU1 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ERFE.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP677939HU1 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ERFE.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
ERFE
Uniprot No.
Research Area
Others
Alternative Names
Complement C1q tumor necrosis factor-related protein 15; ERFE_HUMAN; Erythroferrone; FAM132B; Myonectin
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
195-354aa
Target Protein Sequence
PGPRAPRVEAAFLCRLRRDALVERRALHELGVYYLPDAEGAFRRGPGLNLTSGQYRAPVAGFYALAATLHVALGEPPRRGPPRPRDHLRLLICIQSRCQRNASLEAIMGLESSSELFTISVNGVLYLQMGQWTSVFLDNASGCSLTVRSGSHFSAVLLGV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
33.4 kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Constructing a plasmid encoding the Human ERFE protein (195-354aa) initiates the general approach for generating the recombinant Human ERFE protein. Transformation of the plasmid into e.coli cells obtains the plasmid-containing e.coli cells, which are cultured and induced for protein expression. A N-terminal 6xHis-SUMO tag is fused to the protein. Subsequently, the protein is purified through affinity purification, and SDS-PAGE analysis is undertaken to confirm the presence and assess the purity of the protein. The protein's purity exceeds 85%.

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Target Background

Function
Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.
Gene References into Functions
  1. From a clinical point of view, erythroferrone could become a useful biological marker of iron metabolism and a therapeutic target. [review] PMID: 28666715
  2. FAM132b expression is upregulated in Congenital Dyserythropoietic Anemia type II. PMID: 27540014
  3. In hemodialysis patients DA and CERA increased levels of ERFE that regulated hepcidin 25 and led to iron mobilization from body stores during erythropoiesis. PMID: 26978524
  4. Myonectin links skeletal muscle to lipid homeostasis in liver and adipose tissue in response to alterations in energy state, revealing a novel myonectin-mediated metabolic circuit. PMID: 22351773
Subcellular Location
Secreted.
Protein Families
Adipolin/erythroferrone family
Database Links

HGNC: 26727

OMIM: 615099

KEGG: hsa:151176

UniGene: Hs.24951

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