Human Protein S100-A4(S100A4) ELISA kit

Human Protein S100-A4(S100A4) ELISA kit

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Human Protein S100-A4(S100A4) ELISA kit

Name: Human Protein S100-A4(S100A4) ELISA kit
Brand: CUSABIO
SKU: CSB-EL020632HU

^Instructions

Code	CSB-EL020632HU
Size	96T,5×96T,10×96T
Trial Size
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Target Data

Target Name

S100 calcium binding protein A4

Alternative Names

18A2 ELISA Kit; 42A ELISA Kit; calcium Placental protein ELISA Kit; Calvasculin ELISA Kit; CAPL ELISA Kit; Fibroblast specific protein 1 (FSP1) ELISA Kit; Fibroblast specific protein 1 ELISA Kit; Fibroblast specific protein ELISA Kit; FSP1 ELISA Kit; Leukemia multidrug resistance associated protein ELISA Kit; Malignant transformation suppression 1 (MTS1) ELISA Kit; Malignant transformation suppression 1 ELISA Kit; Metastasin ELISA Kit; MTS1 ELISA Kit; OTTHUMP00000015467 ELISA Kit; OTTHUMP00000015468 ELISA Kit; P9KA ELISA Kit; PEL98 ELISA Kit; Placental calcium-binding protein ELISA Kit; Protein Mts1 ELISA Kit; Protein S100 A4 ELISA Kit; Protein S100-A4 ELISA Kit; S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) ELISA Kit; S100 calcium binding protein A4 ELISA Kit; S100 calcium-binding protein A4 ELISA Kit; S100a4 ELISA Kit; S10A4_HUMAN ELISA Kit

Abbreviation

S100A4

Uniprot No.

P26447

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.9 ng/mL-60 ng/mL

Sensitivity

0.225 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human S100A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-96
1:2	Average %	93
	Range %	82-101
1:4	Average %	97
	Range %	93-104
1:8	Average %	93
	Range %	86-98

Recovery

The recovery of human S100A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-105
EDTA plasma (n=4)	100	95-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.654	2.587	2.621	2.471
30	2.216	2.115	2.166	2.016
15	1.369	1.406	1.388	1.238
7.5	0.689	0.715	0.702	0.552
3.75	0.385	0.367	0.376	0.226
1.8	0.288	0.279	0.284	0.134
0.9	0.216	0.206	0.211	0.061
0	0.156	0.144	0.150

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

7-14 working days

Decreasing of S100A4 in bovine endometritis in vivo and in vitro Y Li,Theriogenology,2020
Differential association between S100A4 levels and insulin resistance in prepubertal children and adult subjects with clinically severe obesity Taxerås S D, et al,Obesity Science & Practice,2019
Towards a circulating marker of hepato-visceral fat excess: S100A4 in adolescent girls with polycystic ovary syndrome-Evidence from randomized clinical trials Malpique R, et al,Pediatric Obesity,2019

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.