Human Protein S100-A4(S100A4) ELISA kit

Instructions
Code CSB-EL020632HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name S100 calcium binding protein A4
Alternative Names 18A2 ELISA Kit; 42A ELISA Kit; calcium Placental protein ELISA Kit; Calvasculin ELISA Kit; CAPL ELISA Kit; Fibroblast specific protein 1 (FSP1) ELISA Kit; Fibroblast specific protein 1 ELISA Kit; Fibroblast specific protein ELISA Kit; FSP1 ELISA Kit; Leukemia multidrug resistance associated protein ELISA Kit; Malignant transformation suppression 1 (MTS1) ELISA Kit; Malignant transformation suppression 1 ELISA Kit; Metastasin ELISA Kit; MTS1 ELISA Kit; OTTHUMP00000015467 ELISA Kit; OTTHUMP00000015468 ELISA Kit; P9KA ELISA Kit; PEL98 ELISA Kit; Placental calcium-binding protein ELISA Kit; Protein Mts1 ELISA Kit; Protein S100 A4 ELISA Kit; Protein S100-A4 ELISA Kit; S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) ELISA Kit; S100 calcium binding protein A4 ELISA Kit; S100 calcium-binding protein A4 ELISA Kit; S100a4 ELISA Kit; S10A4_HUMAN ELISA Kit
Abbreviation S100A4
Uniprot No. P26447
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.9 ng/mL-60 ng/mL
Sensitivity 0.225 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human S100A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-96
1:2Average %93
Range %82-101
1:4Average %97
Range %93-104
1:8Average %93
Range %86-98
Recovery
The recovery of human S100A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-105
EDTA plasma (n=4)10095-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
602.654 2.587 2.621 2.471
302.216 2.115 2.166 2.016
151.369 1.406 1.388 1.238
7.50.689 0.715 0.702 0.552
3.750.385 0.367 0.376 0.226
1.80.288 0.279 0.284 0.134
0.90.216 0.206 0.211 0.061
00.156 0.144 0.150  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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