Human Protein-glutamine gamma-glutamyltransferase 4(TGM4) ELISA kit

Code CSB-EL023464HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
transglutaminase 4 (prostate)
Alternative Names
Dorsal prostate transglutaminase ELISA Kit; Dorsal protein 1 ELISA Kit; Dp1 ELISA Kit; Fibrinoligase ELISA Kit; hTGP ELISA Kit; Prostate specific transglutaminase ELISA Kit; Prostate transglutaminase ELISA Kit; Prostate-specific transglutaminase ELISA Kit; Protein glutamine gamma glutamyltransferase 4 ELISA Kit; Protein-glutamine gamma-glutamyltransferase 4 ELISA Kit; TG(P) ELISA Kit; TGase P ELISA Kit; TGase-4 ELISA Kit; TGase4 ELISA Kit; Tgm4 ELISA Kit; TGM4_HUMAN ELISA Kit; TGP ELISA Kit; Transglutaminase 4 (prostate) ELISA Kit; Transglutaminase 4 ELISA Kit; Transglutaminase P ELISA Kit; Transglutaminase, prostate ELISA Kit; Transglutaminase-4 ELISA Kit
Abbreviation
TGM4
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
50 pg/mL-3200 pg/mL
Sensitivity
12.5 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TGM4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 81-97
1:2 Average % 90
Range % 83-100
1:4 Average % 95
Range % 89-110
1:8 Average % 95
Range % 91-99
Recovery
The recovery of human TGM4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-104
EDTA plasma (n=4) 93 88-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3200 2.724 2.705 2.715 2.622
1600 2.058 2.017 2.038 1.945
800 1.382 1.369 1.376 1.283
400 0.907 0.924 0.916 0.823
200 0.581 0.584 0.583 0.490
100 0.331 0.339 0.335 0.242
50 0.222 0.220 0.221 0.128
0 0.091 0.095 0.093  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human TGM4 ELISA Kit was designed for the quantitative measurement of Human TGM4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 50 pg/mL-3200 pg/mL and the sensitivity is 12.5 pg/mL.

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Target Background

Function
(From Uniprot)
Associated with the mammalian reproductive process. Catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract.
Gene References into Functions
  1. TGM4 is a prostatic secretory molecule with critical role in male reproduction. PMID: 26084804
  2. indicated that transglutaminase 4 may serve as a potential predictor of biochemical recurrence of prostate cancer PMID: 23974364
  3. Evidence that the retinoic acid receptor gamma plays a major role in the regulation of the hTGP gene and that presence of the androgen receptor, but not its transcriptional transactivation activity, is critical for hTGP transcription. PMID: 22362749
  4. CDKN2A, GATA3, CREBBP, ITGA2, NBL1 and TGM4 were down-regulated in the prostate carcinoma glands compared to the corresponding normal glands PMID: 21743959
  5. The presence of TGase-4 has a biological impact on a prostate cancer cell's response to MDA-7. PMID: 21524313
  6. TGase-4 expression is intrinsically linked to the activation of RON in prostate cancer cells and that this autoactivation of RON contributes to the increased cell motility in TGase-4 expressing cells. PMID: 20596668
  7. The expression status of human transglutaminase 4 in benign prostate hyperplasia (BPH) and prostate cancer was analyzed. PMID: 20177144
  8. transglutaminase 4 has a relatively wide profile of expression in human cancer cell lines PMID: 17552366
  9. TGase-4 plays a pivotal role in the interaction between endothelial cells and prostate cancer cells, an action which is independent of the ROCK pathway. PMID: 18983858

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Protein Families
Transglutaminase superfamily, Transglutaminase family
Tissue Specificity
Prostate.
Database Links

HGNC: 11780

OMIM: 600585

KEGG: hsa:7047

STRING: 9606.ENSP00000296125

UniGene: Hs.438265

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