Human Resistin-like Molecule β (RELM-β) ELISA Kit

Code CSB-E13852h
Size 96T,5×96T,10×96T
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Product Details

Target Name
resistin like beta
Alternative Names
C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 2 ELISA Kit; CCGR ELISA Kit; Colon and small intestine specific cysteine rich protein ELISA Kit; Colon and small intestine-specific cysteine-rich protein ELISA Kit; Colon carcinoma related gene protein ELISA Kit; Colon carcinoma-related gene protein ELISA Kit; Cysteine rich secreted A12 alpha like protein 1 ELISA Kit; Cysteine rich secreted protein A12 alpha like 1 ELISA Kit; Cysteine rich secreted protein FIZZ2 ELISA Kit; Cysteine-rich secreted protein A12-alpha-like 1 ELISA Kit; Cysteine-rich secreted protein FIZZ2 ELISA Kit; FIZZ1 ELISA Kit; FIZZ2 ELISA Kit; Found in inflammatory zone 1 ELISA Kit; HXCP2 ELISA Kit; RELM beta ELISA Kit; RELMb ELISA Kit; RELMbeta ELISA Kit; Resistin like beta ELISA Kit; Resistin like protein beta ELISA Kit; Resistin-like beta ELISA Kit; RETNB_HUMAN ELISA Kit; RETNL2 ELISA Kit; Retnlb ELISA Kit; XCP2 ELISA Kit
Abbreviation
RETNLB
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates
Detection Range
47 pg/mL-3000 pg/mL
Sensitivity
11.7 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human RELM-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 85
Range % 80-90
1:2 Average % 96
Range % 92-100
1:4 Average % 90
Range % 85-94
1:8 Average % 92
Range % 88-96
Recovery
The recovery of human RELM-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 95-106
EDTA plasma (n=4) 95 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.210 2.349 2.280 2.121
1500 1.647 1.713 1.680 1.521
750 1.129 1.092 1.111 0.952
375 0.701 0.674 0.688 0.529
187.5 0.438 0.457 0.448 0.289
94 0.352 0.369 0.361 0.202
47 0.224 0.235 0.230 0.071
0 0.156 0.162 0.159  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human RETNLB ELISA Kit was designed for the quantitative measurement of Human RETNLB protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.7 pg/mL.

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Target Background

Function
(From Uniprot)
Probable hormone.
Gene References into Functions
  1. The human mesangial cells with up-regulated and down-regulated expression of RELM-beta increased or decreased significantly at 2-3 days. PMID: 28801998
  2. RELMbeta-overexpression can facilitate invasion and migration of gastric carcinoma cells. PMID: 27001185
  3. RELMbeta levels were increased in Abdominal aortic aneurysm patients in the serum and in the aortic tissue. Increased RELMbeta levels may be involved in the pathogenesis of AAA formation and progress. PMID: 25479529
  4. The data demonstrated that RELM-a is a promising novel biomarker of angiogenesis in patients with gastric cancer. PMID: 25937206
  5. elevated RELM-beta expression in asthmatic airways contributes to airways remodelling at least partly by increasing fibroblast proliferation and differentiation with resulting deposition of extracellular matrix proteins. PMID: 25545115
  6. It is concluded that proper exercise training prevents up-regulation of FIZZ1/RELMalpha induced by cigarette smoking, which may be involved in the mechanism of proper exercise training modulating airway hyperresponsiveness. PMID: 23392702
  7. RELMbeta is abundantly expressed in foam cells within plaques and contributes to atherosclerosis development via lipid accumulation and inflammatory facilitation. PMID: 23702657
  8. Epithelial cell derived Fizz1 transgene is sufficient to increase bone-marrow derived dendritic cells in the lungs. PMID: 22726462
  9. RELM-beta may play an important role not only in animal models of airway remodelling, but also in human airway pathology. PMID: 22758223
  10. RELM-beta has the potential to contribute to airway remodelling in diseases such as asthma by acting on epithelial cells to increase proliferation, mucin and growth factor production, at least partly via ERK/MAPK-PI3K/Akt signalling pathways. PMID: 21828035
  11. Data show that over-expression of RELMbeta abolishes the invasion, metastasis and angiogenesis of gastric cancer cells in vitro. PMID: 22371635
  12. High RELMbeta is associated with colorectal cancer. PMID: 21094111
  13. FIZZ2 is highly induced in lungs of human patients with idiopathic pulmonary fibrosis. PMID: 21602491
  14. Gastric cncer patients showing positive RELMbeta expression had a significantly longer overall survival than those with negative expression (P = 0.001). PMID: 19967544
  15. High levels of RELMbeta can be detected in the stool of mice and humans, where it exists as a homodimer under nonreducing conditions. PMID: 14598255
  16. Of the 80 colon cancer patients studied, 65 (81.25%) tested positive for RELM beta, mainly in the cytoplasm of colon mucosa. PMID: 18594973
  17. Results suggest that RELM-beta may be involved in the development of scleroderma-associated pulmonary hypertension. PMID: 19251945
  18. Supernatant from COS cells transfected with the CCRG (RETNLB) expression vector stimulated proliferation of colon cancer cells, which supports the growth factor nature of the RETNLB gene. PMID: 12224133

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Subcellular Location
Secreted.
Protein Families
Resistin/FIZZ family
Tissue Specificity
Expressed only in the gastrointestinal tract, particularly the colon.
Database Links

HGNC: 20388

OMIM: 605645

KEGG: hsa:84666

STRING: 9606.ENSP00000295755

UniGene: Hs.307047

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