Human Sclerostin(SOST) ELISA Kit

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Target Data

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Human Sclerostin(SOST) ELISA Kit

Name: Human Sclerostin(SOST) ELISA Kit
Brand: CUSABIO
SKU: CSB-E13146h

^Instructions

Code	CSB-E13146h
Size	96T,5×96T,10×96T
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Target Data

Target Name

sclerosteosis

Alternative Names

VBCH, sclerostin

Abbreviation

SOST

Uniprot No.

Q9BQB4

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-92
1:2	Average %	95
	Range %	90-100
1:4	Average %	96
	Range %	90-101
1:8	Average %	93
	Range %	88-99

Recovery

The recovery of human SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-99
EDTA plasma (n=4)	90	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	1.932	1.904	1.918	1.808
1000	1.516	1.481	1.499	1.389
500	1.112	1.101	1.107	0.997
250	0.733	0.687	0.710	0.600
125	0.547	0.504	0.526	0.416
62.5	0.329	0.309	0.319	0.209
31.25	0.197	0.181	0.189	0.079
0	0.112	0.107	0.110

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Role of sclerostin and dkk1 in bone remodeling in type 2 diabetic patients Wang N.et al,Endocr Res.,2017
Increased RANKL/OPG Ratio and Sclerostin in Patients withSeptic Shock Maria Bergquist.et al,Clinical Microbiology and Infectious Diseases,2017
Serum sclerostin values are associated with abdominal aortic calcification and predict cardiovascular events in patients with chronic kidney disease stages 3-5D. Wang XR.et al,Nephrology (Carlton).,2016
Paeoniflorin Atttenuates Amyloidogenesis and the Inflammatory Responses in a Transgenic Mouse Model of Alzheimer´s Disease Hong-Ri Zhang. et al,Neurochemical Research,2015
The effect of single extremity-vibration on the serum sclerostin level Halil Ibrahim Cakar. et al,Journal of Physical Therapy Science,2015
Serum YKL-40/chitinase 3-like protein 1 level is an independent predictor of atherosclerosis development in patients with obstructive sleep apnoea syndrome Eftal Murat Bakirci. et al,Turk Kardiyol Dern Ars,2015
Effects of sex steroids on serum sclerostin levels during the menstrual cycle. Cidem M et al,Gynecol Obstet Invest,2013

Function	Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Involvement in disease	Sclerosteosis 1 (SOST1); Van Buchem disease (VBCH); Craniodiaphyseal dysplasia autosomal dominant (CDD)
Subcellular Location	Secreted, extracellular space, extracellular matrix
Protein Families	Sclerostin family
Tissue Specificity	Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level).
Database Links	HGNC: 13771 OMIM: 122860 KEGG: hsa:50964 STRING: 9606.ENSP00000301691 UniGene: Hs.349204

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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