Human Sclerostin(SOST) ELISA Kit

Instructions
Code CSB-E13146h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name sclerosteosis
Alternative Names VBCH, sclerostin
Abbreviation SOST
Uniprot No. Q9BQB4
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 80-92  
1:2 Average % 95  
Range % 90-100  
1:4 Average % 96  
Range % 90-101  
1:8 Average % 93  
Range % 88-99  
Recovery
The recovery of human SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 89-99  
EDTA plasma (n=4) 90 85-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 1.932 1.904 1.918 1.808  
1000 1.516 1.481 1.499 1.389  
500 1.112 1.101 1.107 0.997  
250 0.733 0.687 0.710 0.600  
125 0.547 0.504 0.526 0.416  
62.5 0.329 0.309 0.319 0.209  
31.25 0.197 0.181 0.189 0.079  
0 0.112 0.107 0.110    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Involvement in disease Sclerosteosis 1 (SOST1); Van Buchem disease (VBCH); Craniodiaphyseal dysplasia autosomal dominant (CDD)
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Sclerostin family
Tissue Specificity Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level).
Database Links

HGNC: 13771

OMIM: 122860

KEGG: hsa:50964

STRING: 9606.ENSP00000301691

UniGene: Hs.349204

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