Human Sclerostin(SOST) ELISA Kit

Code CSB-E13146h
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9BQB4
Lead Time 3-5 working days
Abbreviation SOST
Protein Biological Process 1 Signaling Pathway
Target Name sclerosteosis
Alias VBCH, sclerostin
Species Homo sapiens (Human)
Protein Biological Process 3 Wnt signaling pathway
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
HGNC 13771
RGD 69358
MGI 1921749
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 80-92  
1:2 Average % 95  
Range % 90-100  
1:4 Average % 96  
Range % 90-101  
1:8 Average % 93  
Range % 88-99  
The recovery of human SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 89-99  
EDTA plasma (n=4) 90 85-96  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 1.932 1.904 1.918 1.808  
1000 1.516 1.481 1.499 1.389  
500 1.112 1.101 1.107 0.997  
250 0.733 0.687 0.710 0.600  
125 0.547 0.504 0.526 0.416  
62.5 0.329 0.309 0.319 0.209  
31.25 0.197 0.181 0.189 0.079  
0 0.112 0.107 0.110    
Still Have Questions? Leave a Message or Start an on-line Chat
Function Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Involvement in disease Sclerosteosis 1 (SOST1); Van Buchem disease (VBCH); Craniodiaphyseal dysplasia autosomal dominant (CDD)
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Sclerostin family
Tissue Specificity Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level).
Database Links

HGNC: 13771

OMIM: 122860

KEGG: hsa:50964

STRING: 9606.ENSP00000301691

UniGene: Hs.349204

Pathway Wnt signaling pathway

Related Products

SOST Antibodies

SOST Antibodies for Human

SOST Antibodies for Homo sapiens (Human)

SOST Proteins

SOST Proteins for Homo sapiens (Human)

Most popular with customers


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 CUSABIO TECHNOLOGY LLC All Rights Reserved.


Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.