Target Name
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3G
Alternative Names
FLJ00014 ELISA Kit; MGC119473 ELISA Kit; Sem2 ELISA Kit; SEM3G_HUMAN ELISA Kit; Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3G ELISA Kit; Sema3g ELISA Kit; Semaphorin 3G ELISA Kit; Semaphorin sem2 ELISA Kit; Semaphorin-3G ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human SEMA3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 97 | | |
| Range % | 91-102 | | |
| 1:2 | Average % | 101 | | |
| Range % | 96-105 | | |
| 1:4 | Average % | 94 | | |
| Range % | 87-98 | | |
| 1:8 | Average % | 99 | | |
| Range % | 91-103 | | |
Recovery
| The recovery of human SEMA3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 87 | 82-91 | | |
| EDTA plasma (n=4) | 97 | 90-100 | | |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | | 40 | 2.005 | 1.941 | 1.973 | 1.824 | | | | 20 | 1.613 | 1.576 | 1.595 | 1.446 | | | | 10 | 1.144 | 1.109 | 1.127 | 0.978 | | | | 5 | 0.746 | 0.733 | 0.740 | 0.591 | | | | 2.5 | 0.544 | 0.524 | 0.534 | 0.385 | | | | 1.25 | 0.383 | 0.372 | 0.378 | 0.229 | | | | 0.625 | 0.275 | 0.269 | 0.272 | 0.123 | | | | 0 | 0.152 | 0.146 | 0.149 | | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Human SEMA3G ELISA Kit was designed for the quantitative measurement of Human SEMA3G protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
