Human Spondin-2 ELISA Kit

Code CSB-E14967h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9BUD6
Lead Time 3-5 working days
Abbreviation SPON2
Protein Biological Process 1 Cell Adhesion
Target Name spondin 2, extracellular matrix protein
Alias DIL-1, DIL1, DKFZp686G21139, FLJ16313, FLJ34460, M-spondin, Mindin,
Species Homo sapiens (Human)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma, cerebrospinal fluid (CSF), cell culture supernates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 11253
RGD 708584
MGI 1923724
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %84
Range %80-88
1:2Average %97
Range %91-100
1:4Average %97
Range %92-104
1:8Average %93
Range %86-98
Recovery
The recovery of human spondin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2001.825 1.897 1.861 1.744
1001.414 1.435 1.425 1.308
500.996 0.981 0.989 0.872
250.692 0.683 0.688 0.571
12.50.423 0.458 0.441 0.324
6.250.295 0.280 0.288 0.171
3.120.206 0.216 0.211 0.094
00.116 0.118 0.117  
References
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Function Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS).
Subcellular Location Secreted, extracellular space, extracellular matrix
Tissue Specificity Expressed in normal lung tissue but not in lung carcinoma cell lines.
Database Links

HGNC: 11253

OMIM: 605918

KEGG: hsa:10417

STRING: 9606.ENSP00000290902

UniGene: Hs.302963

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