Human Spondin-2 ELISA Kit

Code CSB-E14967h
Size 96T,5×96T,10×96T
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Product Details

Target Name
spondin 2, extracellular matrix protein
Alternative Names
Differentially expressed in cancerous and non-cancerous lung cells 1 ELISA Kit; DIL 1 ELISA Kit; DIL-1 ELISA Kit; DIL1 ELISA Kit; DKFZp686G21139 ELISA Kit; FLJ16313 ELISA Kit; FLJ22401 ELISA Kit; FLJ34460 ELISA Kit; M spondin ELISA Kit; Mindin ELISA Kit; ORF Names: ELISA Kit; SPON 2 ELISA Kit; Spon2 ELISA Kit; SPON2_HUMAN ELISA Kit; Spondin 2 ELISA Kit; Spondin 2 extracellular matrix protein ELISA Kit; Spondin-2 ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, cerebrospinal fluid (CSF), cell culture supernates
Detection Range
3.12 ng/mL-200 ng/mL
0.78 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %84
Range %80-88
1:2Average %97
Range %91-100
1:4Average %97
Range %92-104
1:8Average %93
Range %86-98
The recovery of human spondin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
2001.825 1.897 1.861 1.744
1001.414 1.435 1.425 1.308
500.996 0.981 0.989 0.872
250.692 0.683 0.688 0.571
12.50.423 0.458 0.441 0.324
6.250.295 0.280 0.288 0.171
3.120.206 0.216 0.211 0.094
00.116 0.118 0.117  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human SPON2 ELISA Kit was designed for the quantitative measurement of Human SPON2 protein in serum, plasma, cerebrospinal fluid (CSF), cell culture supernates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

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Target Background

(From Uniprot)
Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens. Binds bacterial lipopolysaccharide (LPS).
Gene References into Functions
  1. Egr-1 regulates mindin expression by directly binding to the mindin promoter; mindin suppresses colon cancer progression by blocking angiogenesis. PMID: 28991232
  2. Spondin-2 overexpression contributes to tumor aggressiveness and prognosis in gastric cancer. PMID: 28060752
  3. SPON2 is a transcriptional target of the metastasis gene MACC1. SPON2 induces cell motility in vitro and CRC metastasis in mice. In patients, SPON2 serves as prognostic indicator for CRC metastasis and survival, and might represent a promising target for therapeutic approaches. PMID: 26686083
  4. Data suggest that spondin-2 could be an independent diagnostic and prognostic biomarker of colon cancer. PMID: 25945835
  5. Mindin protects against vascular hyperplasia by suppression of abnormal VSMC proliferation, migration and phenotypic switching in an AKT-dependent manner. PMID: 25751394
  6. Using tissue microarray by immunohistostaining, we found SPON2 to be over-expressed in prostate cancer (PCa). SPON2 staining was more intense in Gleason score sum 7-8 and in PCa patients with metastasis. PMID: 22615945
  7. mindin serves as a novel mediator that protects against cardiac hypertrophy and the transition to heart failure by blocking AKT/GSK3beta and TGF-beta1-Smad signalling. PMID: 21632881
  8. High Mindin is associated with diabetic nephropathy in type 2 diabetes. PMID: 21098016
  9. Spondin 2, which is regulated by T(3), has an important role in cell invasion, cell migration, and hepatoma tumor progression. PMID: 19903741
  10. The structure of the F-spondin domain of mindin was determined at 1.8-A resolution. PMID: 19153605

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Tissue Specificity
Expressed in normal lung tissue but not in lung carcinoma cell lines.
Database Links

HGNC: 11253

OMIM: 605918

KEGG: hsa:10417

STRING: 9606.ENSP00000290902

UniGene: Hs.302963

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