Recombinant Human Spondin-2 (SPON2)

Code CSB-YP874811HU
MSDS
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Source Yeast
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Code CSB-EP874811HU
MSDS
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Source E.coli
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Code CSB-EP874811HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874811HU
MSDS
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Source Baculovirus
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Code CSB-MP874811HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPON2
Uniprot No.
Alternative Names
Differentially expressed in cancerous and non-cancerous lung cells 1; DIL 1; DIL-1; DIL1; DKFZp686G21139; FLJ16313; FLJ22401; FLJ34460; M spondin; Mindin; ORF Names: ; SPON 2; Spon2; SPON2_HUMAN; Spondin 2; Spondin 2 extracellular matrix protein; Spondin-2
Species
Homo sapiens (Human)
Expression Region
27-331
Target Protein Sequence
QPLG GESICSARAL AKYSITFTGK WSQTAFPKQY PLFRPPAQWS SLLGAAHSSD YSMWRKNQYV SNGLRDFAER GEAWALMKEI EAAGEALQSV HEVFSAPAVP SGTGQTSAEL EVQRRHSLVS FVVRIVPSPD WFVGVDSLDL CDGDRWREQA ALDLYPYDAG TDSGFTFSSP NFATIPQDTV TEITSSSPSH PANSFYYPRL KALPPIARVT LVRLRQSPRA FIPPAPVLPS RDNEIVDSAS VPETPLDCEV SLWSSWGLCG GHCGRLGTKS RTRYVRVQPA NNGSPCPELE EEAECVPDNC V
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens. Binds bacterial lipopolysaccharide (LPS).
Gene References into Functions
  1. Egr-1 regulates mindin expression by directly binding to the mindin promoter; mindin suppresses colon cancer progression by blocking angiogenesis. PMID: 28991232
  2. Spondin-2 overexpression contributes to tumor aggressiveness and prognosis in gastric cancer. PMID: 28060752
  3. SPON2 is a transcriptional target of the metastasis gene MACC1. SPON2 induces cell motility in vitro and CRC metastasis in mice. In patients, SPON2 serves as prognostic indicator for CRC metastasis and survival, and might represent a promising target for therapeutic approaches. PMID: 26686083
  4. Data suggest that spondin-2 could be an independent diagnostic and prognostic biomarker of colon cancer. PMID: 25945835
  5. Mindin protects against vascular hyperplasia by suppression of abnormal VSMC proliferation, migration and phenotypic switching in an AKT-dependent manner. PMID: 25751394
  6. Using tissue microarray by immunohistostaining, we found SPON2 to be over-expressed in prostate cancer (PCa). SPON2 staining was more intense in Gleason score sum 7-8 and in PCa patients with metastasis. PMID: 22615945
  7. mindin serves as a novel mediator that protects against cardiac hypertrophy and the transition to heart failure by blocking AKT/GSK3beta and TGF-beta1-Smad signalling. PMID: 21632881
  8. High Mindin is associated with diabetic nephropathy in type 2 diabetes. PMID: 21098016
  9. Spondin 2, which is regulated by T(3), has an important role in cell invasion, cell migration, and hepatoma tumor progression. PMID: 19903741
  10. The structure of the F-spondin domain of mindin was determined at 1.8-A resolution. PMID: 19153605

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Tissue Specificity
Expressed in normal lung tissue but not in lung carcinoma cell lines.
Database Links

HGNC: 11253

OMIM: 605918

KEGG: hsa:10417

STRING: 9606.ENSP00000290902

UniGene: Hs.302963

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