TPK1 Antibody

Datasheet

Code	CSB-PA024103ESR2HU
Size	US$166
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Image	Western blot All lanes: TPK1 antibody at 0.78μg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 28, 14 kDa Observed band size: 28 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA024103ESR2HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA024103ESR2HU at dilution of 1:100 Immunofluorescent analysis of PC-3 cells using CSB-PA024103ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Immunoprecipitating TPK1 in Mouse kidney tissue Lane 1: Rabbit control IgG instead of CSB-PA024103ESR2HU in Mouse kidney tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA024103ESR2HU (8μg) + Mouse kidney tissue (500μg) Lane 3: Mouse kidney tissue (10μg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TPK1 Polyclonal antibody

Uniprot No.

Q9H3S4

Target Names

TPK1

Alternative Names

hTPK1 antibody; Placental protein 20 antibody; PP20 antibody; Thiamin pyrophosphokinase 1 antibody; Thiamine diphosphokinase antibody; Thiamine kinase antibody; Thiamine pyrophosphokinase 1 antibody; THMD5 antibody; TPK1 antibody; TPK1_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Thiamin pyrophosphokinase 1 protein (1-243AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.

Gene References into Functions

Mutation analysis of TPK1 uncovered three missense, one splice-site, and one frameshift mutation resulting in decreased TPK protein levels PMID: 22152682
findings indicate the importance of the Sp1 transcription factor cis-element in regulating the human thiamin pyrophosphokinase gene (hTPK1) gene expression PMID: 16262001
Genomic variations in either the fetal or maternal TPK1 gene could contribute to variability of birth weight in normal humans. PMID: 17295612
This is the first report on the primary sequence for mammalian thiamin pyrophosphokinase, a protein that catalyzes the pyrophosphorylation of thiamin with adenosine 5'-triphosphate to form thiamin pyrophosphate. PMID: 10567383

Involvement in disease

Thiamine metabolism dysfunction syndrome 5, episodic encephalopathy type (THMD5)

Protein Families

Thiamine pyrophosphokinase family

Tissue Specificity

Detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues.

Database Links

HGNC: 17358

OMIM: 606370

KEGG: hsa:27010

STRING: 9606.ENSP00000353165

UniGene: Hs.660232

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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