Human Thyroid Stimulating Hormone,TSH ELISA Kit

Code CSB-E05114h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details


This Human TSH ELISA Kit was designed for the quantitative measurement of Human TSH protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.3 uIU/mL-12 uIU/mL and the sensitivity is 0.15 μIU/mL.

Target Name Thyroid Stimulating Hormone,TSH
Alternative Names TSHBThyrotropin subunit beta ELISA kit; Thyroid-stimulating hormone subunit beta ELISA kit; TSH-B ELISA kit; TSH-beta ELISA kit; Thyrotropin beta chain ELISA kit; Thyrotropin alfa ELISA kit
Abbreviation TSH
Uniprot No. P01222
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 0.3 uIU/mL-12 uIU/mL
Sensitivity 0.15 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %96
Range %92-104
1:2Average %94
Range %91-98
1:4Average %102
Range %98-106
1:8Average %88
Range %82-94
The recovery of human TSHspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)10398-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
122.219 2.354 2.287
61.215 1.254 1.235
30.648 0.664 0.656
1.50.361 0.354 0.358
0.60.155 0.158 0.157
0.30.078 0.079 0.079
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Indispensable for the control of thyroid structure and metabolism.
Gene References into Functions
  1. Serum macro TSH level is associated with sleep quality in patients with cardiovascular risks. PMID: 28287185
  2. present the cases of two siblings with a novel mutation of TSHB. One of them was c.40A>G (rs10776792) which is a very common variation that is also seen in healthy individuals, the other was c.94G>A at codon 32 of exon 2 which resulted in a change from glutamic acid to lysine (p.E32K PMID: 28515030
  3. The serum levels of IL-8, MIP-1 alpha, MIP-1 beta, MMP-8, Resistin, FLRG, and BCAM were significantly higher in breast cancer patients, but LAP and TSH-beta levels were lower. PMID: 26898119
  4. results provide novel evidence of TSHB as a paracrine factor that is modulated in parallel with cholesterol metabolism in human adipose tissue PMID: 28646016
  5. In hyperthyroidism, bone marrow resident macrophages have the potential to exert enhanced osteoprotective effects by oversecreting a TSH-beta splice variant. PMID: 27300765
  6. In the context of metabolic syndrome, a higher TSH within the euthyroid range confers an increased leptin/adiponectin ratio, a proposed marker of atherosclerosis susceptibility and adipocyte dysfunction. PMID: 28077136
  7. the distribution of 1(st) trimester TSH and evaluate its association with perinatal outcomes and future development of maternal thyrotoxicosis, is reported. PMID: 27351808
  8. Data suggest that the subtle thyroid-stimulating hormone (TSH) reduction could possibly reflect minor hypothalamic-pituitary damage. PMID: 26432979
  9. analysis of a single nucleotide substitution in TSHbeta related to clinical euthyroidism [family case report] PMID: 25950606
  10. Findings defined the immune-derived functional TSHb splice variant that resided in the thyroid of patients with Hashimoto's thyroiditis (HT), which exerted the unique effects on the pathogenesis of the disease. PMID: 26170068
  11. Serum TSH was associated with pulse wave velocity on population level when adjusted with age and sex. PMID: 25185682
  12. Higher TSH levels are associated with less favorable lipid levels in children PMID: 25781359
  13. In cases with isolated central congenital hypothyroidism and undetectably low TSH serum concentrations, a TSHbeta gene deletion should be considered PMID: 25012771
  14. Epistasis between single nucleotide polymorphisms within the TSHB and ADAMTS16 genes may increase the risk of premature ovarian failure in Korean women. PMID: 24366283
  15. High Thyroid Stimulating Hormone levels are associated with papillary thyroid carcinoma. PMID: 24595799
  16. bTSH Induces IL-6 Protein and mRNA in Orbital Fibroblasts and Fibrocytes PMID: 24086448
  17. The use of these age-specific reference intervals for TSH, especially in those over 70 years old, would result in the reclassification of many TSH results from "abnormal" to "normal" and avoid unnecessary treatment. PMID: 23345094
  18. In Hashimoto's thyroiditis the hTSHbeta splice variant exists in human serum and dimerises with TSHalpha. PMID: 22752807
  19. Data suggest that a progressive increase in TSH are predictive factors for thyroid failure in Hashimoto's thyroiditis (HT) patients. PMID: 21981142
  20. Data suggest that likelihood of papillary thyroid carcinoma is reduced when serum TSH is lower, as in thyroid autonomy, and increased when serum TSH is higher, as in thyroid autoimmunity. [Meta-Analysis; REVIEW] PMID: 22278420
  21. Studies indicate that immune system-derived TSH, in particular, a splice variant of TSHbeta that is preferentially made by cells of the immune system, is produced by a subset of hematopoietic cells that traffic to the thyroid. PMID: 20826543
  22. Results suggest that a serum TSH concentration at the lower end of the reference range may be associated with low BMD in men. PMID: 20455884
  23. Novel mutations of the TSH-beta subunit gene underlying congenital central hypothyroidism undetectable in neonatal TSH screening. PMID: 20534762
  24. we describe two new sibships with isolated congenital central hypothyroidism due to two different homozygous TSHbeta gene mutations (c.Q49X and c.C105fs114X). PMID: 20553196
  25. TSHbeta variants C105Vfs114X and Q49X are the most frequent cause of this severe disorder in Europe, now for the first time observed in compound heterozygous state. PMID: 15297803
  26. Data suggest that the expression of Pit-1 in cells of the alpha SU-based gonadotropin cell lineage might also lead to the expression of growth hormone, prolactin, and thyroid-stimulating hormone beta subunit. PMID: 16133148
  27. N-CoR and TRbeta cooperate in the regulation of the TSHbeta gene and this ligand-dependent repression is mediated by the LXXLL motif in N-CoR PMID: 16216492
  28. Serum TSH is frequently suppressed after treatment with antithyroid drugs. PMID: 17954417
  29. In a cohort of pregnant women without overt thyroid dysfunction, the risk of child loss increased with higher levels of maternal TSH. PMID: 19273570
  30. Human pituitary, thyroid, and mononuclear leukocytes express a TSHbeta isoform that is analogous to the mouse TSHbeta isoform, consisting of a 27 nucleotide portion of intron 2 and all of exon 3, coding for 71.2% of the native human TSHbeta polypeptide. PMID: 19364510

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Subcellular Location Secreted.
Protein Families Glycoprotein hormones subunit beta family
Database Links

HGNC: 12372

OMIM: 188540

KEGG: hsa:7252

STRING: 9606.ENSP00000256592

UniGene: Hs.406687

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