Code | CSB-E04725h-IS |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:50 | Average % | 99 | ||||
Range % | 92-106 | |||||
1:100 | Average % | 92 | ||||
Range % | 85-99 | |||||
1:200 | Average % | 89 | ||||
Range % | 84-94 | |||||
1:400 | Average % | 92 | ||||
Range % | 86-98 |
The recovery of human TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 100 | 95-106 | ||||
EDTA plasma (n=4) | 93 | 88-99 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
1500 | 2.690 | 2.638 | 2.664 | 2.491 | |||
750 | 2.196 | 2.147 | 2.171 | 1.999 | |||
375 | 1.630 | 1.608 | 1.619 | 1.446 | |||
187.5 | 1.061 | 1.039 | 1.050 | 0.877 | |||
94 | 0.602 | 0.631 | 0.617 | 0.444 | |||
47 | 0.428 | 0.403 | 0.416 | 0.243 | |||
23.5 | 0.246 | 0.234 | 0.240 | 0.067 | |||
0 | 0.175 | 0.171 | 0.173 |
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