Human Transgelin-2(TAGLN2) ELISA kit

Code CSB-EL023106HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name transgelin 2
Alternative Names CDABP0035 ELISA Kit; epididymis tissue protein Li 7e ELISA Kit; HA1756 ELISA Kit; KIAA0120 ELISA Kit; SM22-alpha homolog ELISA Kit; TAGL2_HUMAN ELISA Kit; Tagln2 ELISA Kit; Transgelin-2 ELISA Kit
Abbreviation TAGLN2
Uniprot No. P37802
Species Homo sapiens (Human)
Sample Types serum, plasma, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TAGLN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %87-92
1:2Average %101
Range %98-103
1:4Average %92
Range %85-96
1:8Average %99
Range %93-102
Recovery
The recovery of human TAGLN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9592-98
EDTA plasma (n=4)9692-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1OD2AverageCorrected
202.398 2.367 2.383 2.228
101.672 1.576 1.624 1.469
51.071 1.062 1.067 0.912
2.50.624 0.618 0.621 0.466
1.250.427 0.411 0.419 0.264
0.6250.319 0.306 0.313 0.158
0.3120.251 0.240 0.246 0.091
00.156 0.154 0.155  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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