| Description |
This Human TAGLN2 ELISA Kit was designed for the quantitative measurement of Human TAGLN2 protein in serum, plasma, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL . |
| Target Name |
transgelin 2 |
| Alternative Names |
CDABP0035 ELISA Kit; epididymis tissue protein Li 7e ELISA Kit; HA1756 ELISA Kit; KIAA0120 ELISA Kit; SM22-alpha homolog ELISA Kit; TAGL2_HUMAN ELISA Kit; Tagln2 ELISA Kit; Transgelin-2 ELISA Kit |
| Abbreviation |
TAGLN2 |
| Uniprot No. |
P37802 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell lysates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TAGLN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:1 | Average % | 90 | | | Range % | 87-92 | | | 1:2 | Average % | 101 | | | Range % | 98-103 | | | 1:4 | Average % | 92 | | | Range % | 85-96 | | | 1:8 | Average % | 99 | | | Range % | 93-102 | |
|
| Recovery |
| The recovery of human TAGLN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 95 | 92-98 | | | EDTA plasma (n=4) | 96 | 92-99 | | | | | | | | | | | | | | | | | |
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.398 | 2.367 | 2.383 | 2.228 | | | 10 | 1.672 | 1.576 | 1.624 | 1.469 | | | 5 | 1.071 | 1.062 | 1.067 | 0.912 | | | 2.5 | 0.624 | 0.618 | 0.621 | 0.466 | | | 1.25 | 0.427 | 0.411 | 0.419 | 0.264 | | | 0.625 | 0.319 | 0.306 | 0.313 | 0.158 | | | 0.312 | 0.251 | 0.240 | 0.246 | 0.091 | | | 0 | 0.156 | 0.154 | 0.155 | | |
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| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
