Human Tropomyosin alpha-4 chain(TPM4) ELISA kit

Code CSB-EL024108HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
tropomyosin 4
Alternative Names
epididymis secretory protein Li 108 ELISA Kit; HEL-S-108 ELISA Kit; TM30p1 ELISA Kit; TMP 4 ELISA Kit; TPM4 ELISA Kit; TPM4_HUMAN ELISA Kit; Tropomyosin 4 ELISA Kit; Tropomyosin alpha 4 chain ELISA Kit; Tropomyosin alpha-4 chain ELISA Kit; Tropomyosin-4 ELISA Kit; wu:fb06e07 ELISA Kit; zgc:103436 ELISA Kit; zgc:63909 ELISA Kit
Abbreviation
TPM4
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TPM4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 100  
Range % 97-103  
1:2 Average % 85  
Range % 81-89  
1:4 Average % 91  
Range % 85-97  
1:8 Average % 99  
Range % 95-104  
Recovery
The recovery of human TPM4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 82-90  
EDTA plasma (n=4) 94 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1500 2.557 2.597 2.577 2.398  
750 1.916 1.936 1.926 1.747  
375 1.278 1.298 1.288 1.109  
187.5 0.798 0.742 0.770 0.591  
94 0.481 0.499 0.490 0.311  
47 0.367 0.355 0.361 0.182  
23.5 0.264 0.268 0.266 0.087  
0 0.178 0.179 0.179    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human TPM4 ELISA Kit was designed for the quantitative measurement of Human TPM4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

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Target Background

Function
(From Uniprot)
Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.
Gene References into Functions
  1. TPM4 is associated with clinical progression in colon cancer patients and acts as a tumor suppressor in colon cancer cells. PMID: 29455030
  2. Tpm4.1 is essential to maintain cell-cell adhesions and to inhibit abnormal increases in cell migration and invasion, which are important to prevent invasion and metastasis of breast cancer cells. PMID: 28431393
  3. findings demonstrate a nonredundant role for TPM4 in platelet biogenesis in humans and mice and reveal that truncating variants in TPM4 cause a previously undescribed dominant Mendelian platelet disorder. PMID: 28134622
  4. Results show that Tpm4.2 facilitates the transition of NM-2A to the strongly actin-binding myosin-ADP state. PMID: 26847712
  5. Data indicate the upregulation of tropomyosin-4 in infiltrating ductal breast carcinomas (IDCAs) tissues, which may suggest its involvement in breast cancer development. PMID: 23812729
  6. TPM4, PDIA and SRC8 were also localized to the trophoblast cells, further highlighting the importance of these cytoskeletal remodelling proteins in early pregnancy PMID: 21373848
  7. Low TPM4 expression in the cytoplasm is associated with uterine cervix carcinogenesis. PMID: 21119665
  8. Tm4-defined cytoskeleton is a marker of growth and repair/regeneration in response to injury, disease state and stress in skeletal muscle. PMID: 17968984

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Tropomyosin family
Tissue Specificity
Detected in cardiac tissue and platelets, the form found in cardiac tissue is a higher molecular weight than the form found in platelets. Expressed at higher levels in the platelets of hypertensive patients with cardiac hypertrophy than in the platelets o
Database Links

HGNC: 12013

OMIM: 600317

KEGG: hsa:7171

UniGene: Hs.631618

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