Human aryl hydrocarbon receptor,AhR ELISA Kit

Instructions
Code CSB-E09355h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name aryl hydrocarbon receptor
Alternative Names Ah receptor ELISA Kit; AhR ELISA Kit; AHR_HUMAN ELISA Kit; Aromatic hydrocarbon receptor ELISA Kit; Aryl hydrocarbon receptor ELISA Kit; Aryl hydrocarbon receptor precursor ELISA Kit; bHLHe76 ELISA Kit; Class E basic helix loop helix protein 76 ELISA Kit; Class E basic helix-loop-helix protein 76 ELISA Kit; HGNC:348 ELISA Kit
Abbreviation AHR
Uniprot No. P35869
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AhR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-95  
1:2 Average % 102  
Range % 97-107  
1:4 Average % 91  
Range % 85-97  
1:8 Average % 97  
Range % 91-103  
Recovery
The recovery of human AhR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 97 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.231 2.108 2.170 1.988  
500 1.794 1.762 1.778 1.596  
250 1.366 1.367 1.367 1.185  
125 0.861 0.898 0.880 0.698  
62.5 0.632 0.602 0.617 0.435  
31.2 0.481 0.472 0.477 0.295  
15.6 0.357 0.342 0.350 0.168  
0 0.181 0.183 0.182    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1.
Gene References into Functions
  1. Expression of microRNA potentially regulated by AhR and CAR in malignant tumors of the endometrium has been reported. PMID: 30225717
  2. The overexpression and activation of AHR are involved in the early development of skin squamous cell carcinomas. AHR expression correlates with EGFR expression and may influence cell proliferation. PMID: 30144817
  3. These studies identified SNPs that were cis-eQTLs for DEFB1 and AHR and, which were associated with variation in plasma KYN concentrations that were related to severity of major depressive disordersymptoms. PMID: 29317604
  4. rs2470893 SNP, which maps 196 bp from a CYP1A1 promoter XRE, is associated with variations in 3MC-dependent AHR binding and CYP1A1 expression. PMID: 29980579
  5. WASH-mediated AHR expression has a critical function in the maintenance of NKp46+ innate lymphoid cells PMID: 28589939
  6. the protective role of AhR in intestinal barrier function PMID: 29992488
  7. Elevated AhR expression may be involved in the progression of tissue remodeling in chronic rhinosinusitis without nasal polyp without allergic rhinitis similar to TGF-beta1 expression. Conversely, lower AhR expression may be involved in allergic reactions in chronic rhinosinusitis with nasal polyp with allergic rhinitis. PMID: 29183012
  8. These results suggest that D-Kyn increases lung cancer cells to metastasize by activating Ahr. PMID: 29442266
  9. The role of IDO1-IDO2-AHR pathway in the TLR4-induced tolerogenic phenotype in human dendritic cells has been reported. PMID: 28256612
  10. The association between AHR Arg55Lys polymorphism and male infertility risk was not confirmed (Meta-Analysis) PMID: 29427904
  11. A meta-analysis of five sets of data evaluated the association of aryl hydrocarbon receptor 1661G>A with male infertility. PMID: 28712079
  12. IDO decreased glycolysis and glutaminolysis by activating GCN2K, resulting in activation of AMPactivated protein kinase. PMID: 29693118
  13. AHR inhibition or knockdown/knockout consistently reduced human ER-/PR-/Her2- and inflammatory breast cancer cell invasion, migration, and metastasis. This was associated with a decrease in invasion-associated genes (e.g., Fibronectin, VCAM1, Thrombospondin, MMP1) and an increase in CDH1/E-cadherin, previously associated with decreased tumor aggression. PMID: 29735912
  14. This study demonstrated that the regulation of AHR expression by miR124 is critical to the development of inflammatory response in chronic rhinosinusitis with nasal polyps. PMID: 29605298
  15. In human keratinocytes, authors found that perillaldehyde (1) inhibited BaP-induced AHR activation and ROS production, (2) inhibited BaP/AHR-mediated release of the CCL2 chemokine, and (3) activated the NRF2/HO1 antioxidant pathway. PMID: 29643980
  16. Infants born to women with specific AHR and XRCC1 genotypes may have higher genetic risks for birth weight reduction PMID: 28893607
  17. ITE suppresses growth of human pulmonary artery endothelial cells independent of AhR PMID: 29140133
  18. High AHR expression is associated with nerve sheath tumorigenesis. PMID: 29351992
  19. IRES (ribosome entry site)activity is increased in the PTX-resistant ovarian cancer cells in which AHR protein expression was also enhanced. These results strongly suggest an important role for AHR ribosome entry site (IRES)-dependent translation mechanism in cancer cell response to paclitaxel treatment PMID: 29048649
  20. AhR activation by FICZ reduces FcepsilonRI and upregulates IDO expression in Langerhans cells. This AhR-mediated anti-inflammatory feedback mechanism may dampen the allergen-induced inflammation in atopic dermatitis. PMID: 28376268
  21. Report yeast estrogen screen for androgen- and estrogen-receptor mediated activities of 4-hydroxytestosterone, 4-hydroxyandrostenedione and their human metabolites. PMID: 29702199
  22. Report AhR-dependent transcriptional activity during acute exposure of lung cells to distinct types of environmental pollutants. PMID: 29704546
  23. The nuclear localization signal (NLS) is closed in the absence of a ligand, the structure of AhR will be changed in the presence of a ligand, which leads to NLS open, result in the nuclear translocation of AhR. PMID: 29092071
  24. Study provides evidence that AhR plays a critical role in polycyclic aromatic hydrocarbon-mediated DNA damage. PMID: 28886471
  25. in transcriptionally active heterodimer with ARNT, which recognizes the dioxin response element (DRE) in the promoter of downstream genes, right next to the DRE-docking sites is the extensive dimerization surface that is more hydrophobic than other surface areas, indicating that the dimerization interface of the AHR transcription complex is largely dictated by hydrophobic contacts PMID: 28396409
  26. AHR signaling is essential for activin A induced regulatory T cells that restrain asthmatic responses. PMID: 28320933
  27. Aryl hydrocarbon receptor activation induced by FICZ and Glyteer increased the expression of OVOL1 and Filaggrin in a dose- and time-dependent manner in normal human epidermal keratinocytes. PMID: 28703805
  28. Study shows the Q-rich/PST domain of the aryl hydrocarbon receptor regulates both ligand-induced nuclear transport and nucleocytoplasmic shuttling. PMID: 27535013
  29. Data suggest that response of bronchial epithelial cells to environmental carcinogen benzo[a]pyrene includes activation of AhR/Src/ERK signaling, CYP1A1 induction, and formation of stable DNA adducts. (AhR = aryl hydrocarbon receptor; Src = Src proto-oncogene kinase; ERK = extracellular signal-regulated kinases; CYP1A1 = cytochrome P450 family 1 subfamily A member 1) PMID: 29545172
  30. These data support a mechanistic pathway for the putative tumour suppressive role of AHR specifically in pituitary adenomas, possibly through its role as a cell cycle co-regulator, even in the absence of exogenous ligands. PMID: 28649092
  31. upregulation of mRNA and protein expression is associated with the pathogenesis of unexplained miscarriage PMID: 28503784
  32. The AhR physiological role in cancer [review] PMID: 28508231
  33. Our findings suggest that AhR-mediated IL-1beta regulation in cerebral endothelium could induce BBB breakdown and contribute to the pathogenesis of a variety of neurologic disorders. PMID: 28285151
  34. Ultraviolet B rays suppressed SIRT1 expression by activating AhR, and subsequently inhibited DNMT1 activity in CD4+ T cells from systemic lupus erythematosus patients. PMID: 28336124
  35. AHR regulated cell proliferation and tumorigenesis by directly targeting and activating HDAC8 expression in hepatocellular carcinoma cells. PMID: 27283490
  36. Human CCL1 gene is selectively targeted by AhR in M(IL-4) macrophage. IL-4-induced epigenetic modification potentiates AhR-mediated CCL1 expression. PMID: 27888289
  37. Moreover, time course and promoter activity assays suggest that TIPARP and TIPARP-AS1 work in concert to regulate AHR signaling. Collectively, these data show an added level of complexity in the AHR signaling cascade which involves lncRNAs, whose functions remain poorly understood. PMID: 29274782
  38. Utilising a three-dimensional lymph endothelial cell (LEC) monolayer & MDA-MB231 breast cancer cell spheroid co-culture model in combination with knock-down approach allowed elucidation of the molecular/biochemical basis of AHR/CYP1A1-induced tumour breaching through the LEC barrier. PMID: 28171546
  39. breast cancer ER status likely influences AhR activity involved in these signaling pathways. The mechanisms involved in AhR activation and target gene expression in breast cancers are also discussed PMID: 29320557
  40. AhR modulates hyperoxic lung injury by regulating the genes that are necessary for cell proliferation and inflammation. PMID: 27103661
  41. This study of human fibroblasts bearing endogenous heterozygous AIP mutations and transfected pituitary GH3 cells shows that AIP mutations affect the AIP protein level and alter AhR transcriptional activity in a gene- and tissue-dependent manner. PMID: 27080473
  42. We demonstrated that infants whose mothers smoked during pregnancy with the combination of AHR, CYP1A1, and XRCC1 polymorphisms had lower birth size. PMID: 27592400
  43. A low AHR-expression level in human lung cancer tissues is significantly associated with its malignancy.Cytoplasmic, resting AHR protein acts as an epithelial-mesenchymal transition suppressor via a non-genomic pathway. PMID: 27752740
  44. Aryl hydrocarbon receptor signaling modifies Toll-like receptor-regulated responses in human dendritic cells. PMID: 27783115
  45. These findings identify a role for pattern recognition receptor (PRR) signaling in regulating the AHR response through selective down-regulation of Cyp1 expression in human monocytes and macrophages. PMID: 29018148
  46. The results of the present study suggest that AhR stimulates estrogen-dependent progression of breast carcinoma by inducing aromatase expression under some conditions. These results provide new insights on the possible roles of environmental toxins in breast cancer development. PMID: 27900579
  47. AHR, CYP1A1, CYP1A2, or CYP1B1 variants associated with head-and-neck squamous cell carcinoma risk in smokers. PMID: 27894333
  48. Low AHR expression is associated with Prostate Cancer. PMID: 28972393
  49. activation of the aryl hydrocarbon receptor using the non-toxic agonist 2-(1'H-indole-3'-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE) induces human regulatory t cells. PMID: 27783946
  50. this study demonstrates a role for the AHR in regulating human B cell development, and it suggests that transcriptional alterations of EBF1 by the AHR are involved in the underlying mechanism PMID: 28978690

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Subcellular Location Cytoplasm, Nucleus
Tissue Specificity Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.
Database Links

HGNC: 348

OMIM: 600253

KEGG: hsa:196

STRING: 9606.ENSP00000242057

UniGene: Hs.171189

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