Human coagulation factor Ⅶ,FⅦ ELISA Kit

Instructions
Code CSB-E12909h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name coagulation factor VII (serum prothrombin conversion accelerator)
Alternative Names , FVII coagulation protein|coagulation factor VII|eptacog alfa
Abbreviation F7
Uniprot No. P08709
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Blood Coagulation
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FⅦ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %92
Range %87-95
1:2000Average %104
Range %99-107
1:4000Average %92
Range %85-96
1:8000Average %97
Range %93-100
Recovery
The recovery of human FⅦ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-97
EDTA plasma (n=4)9591-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.664 2.652 2.658 2.559
102.341 2.302 2.322 2.223
51.900 1.898 1.899 1.800
2.51.120 1.132 1.126 1.027
1.250.653 0.634 0.644 0.545
0.6250.376 0.366 0.371 0.272
0.3120.247 0.232 0.240 0.141
00.101 0.097 0.099  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
Involvement in disease Factor VII deficiency (FA7D)
Subcellular Location Secreted
Protein Families Peptidase S1 family
Tissue Specificity Plasma.
Database Links

HGNC: 3544

OMIM: 227500

KEGG: hsa:2155

STRING: 9606.ENSP00000364731

UniGene: Hs.36989

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.