Human coagulation factor Ⅶ,FⅦ ELISA Kit

Code CSB-E12909h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P08709
Lead Time 3-5 working days
Abbreviation F7
Protein Biological Process 1 Blood Coagulation
Target Name coagulation factor VII (serum prothrombin conversion accelerator)
Alias , FVII coagulation protein|coagulation factor VII|eptacog alfa
Species Homo sapiens (Human)
Protein Biological Process 3 Blood coagulation
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Blood Coagulation
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Alternative splicing of this gene results in 2 transcripts. Defects in this gene can cause coagulopathy.
HGNC 3544
RGD 628678
MGI 109325
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FⅦ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %92
Range %87-95
1:2000Average %104
Range %99-107
1:4000Average %92
Range %85-96
1:8000Average %97
Range %93-100
Recovery
The recovery of human FⅦ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-97
EDTA plasma (n=4)9591-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.664 2.652 2.658 2.559
102.341 2.302 2.322 2.223
51.900 1.898 1.899 1.800
2.51.120 1.132 1.126 1.027
1.250.653 0.634 0.644 0.545
0.6250.376 0.366 0.371 0.272
0.3120.247 0.232 0.240 0.141
00.101 0.097 0.099  
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Function Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
Involvement in disease Factor VII deficiency (FA7D)
Subcellular Location Secreted
Protein Families Peptidase S1 family
Tissue Specificity Plasma.
Database Links

HGNC: 3544

OMIM: 227500

KEGG: hsa:2155

STRING: 9606.ENSP00000364731

UniGene: Hs.36989

Pathway Complement and coagulation cascades

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