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| Code | CSB-E17834h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human GOT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 80-89 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 88-97 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 91-100 | ||||||
| 1:8 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| The recovery of human GOT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 98-109 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human GOT2 ELISA Kit is engineered for accurate measurement of human GOT2 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the human GOT2 content in the sample. The color intensity is positively correlated with GOT2 content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
GOT2 is a mitochondrial glutamate oxaloacetate transaminase that catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). It facilitates the cellular uptake of long-chain free fatty acids. GOT2 plays a key role in amino acid metabolism, intracellular NAD(H) redox balance, as well as metabolite exchange between mitochondria and cytosol. Studies have shown that loss of GOT2 disturbs redox homeostasis and halts the proliferation of pancreatic ductal adenocarcinoma (PDA) cells in vitro.
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