24T ELISA Kit Trial Size (Only USD$150/ kit)
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The human HGF ELISA kit (CSB-E04573h) is designed for the quantitative measurement of human HGF protein in serum, plasma, or tissue homogenates. It quantitates human HGF with 3.9 pg/ml sensitivity and shows excellent specificity for human HGF. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated HGF antibody that recognizes the analyte bound by the immobilized HGF antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of HGF in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the human HGF in the sample.
HGF is a cytokine with pleiotropic functions during wound healing and repair. In the bleomycin model of lung fibrosis, HGF was demonstrated to exert anti-fibrotic effects and associated with improved survival and proliferation of epithelial cells and reduction of myofibroblast accumulation. HGF can induce epithelial-mesenchymal conversion of epithelial cells in culture, with the dissociated cells becoming highly motile. HGF/c-Met signaling pathway has been involved in different processes including development, organ regeneration, and cancer as well as in medulloblastoma pathogenesis.
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