Recombinant Human Hepatocyte growth factor(HGF) (Active)

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Code CSB-AP003681HU
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity The ED50 as determined by its ability to induce IL-11 secretion by Saos?2 human osteosarcoma cells is less than 10 ng/ml.
Target Names HGF
Uniprot No. P14210
Research Area Cancer
Alternative Names DFNB39; F TCF; Fibroblast derived tumor cytotoxic factor; Hepatocyte growth factor (hepapoietin A; scatter factor); Hepatocyte growth factor; Hepatocyte growth factor beta chain; Hepatocyte growth factor precursor; Hepatopoietin A; Hepatopoietin-A; Hgf; HGF_HUMAN; HGFB; HPTA; Lung fibroblast derived mitogen; OTTHUMP00000161349; OTTHUMP00000206710; OTTHUMP00000206711; OTTHUMP00000206712; OTTHUMP00000206713; OTTHUMP00000206730; Scatter factor; SF
Species Homo sapiens (Human)
Source Mammalian cell
Expression Region 32-728aa
Complete Sequence QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS
Mol. Weight 79.7 kDa
Protein Length Full Length of Mature Protein
Tag Info C-terminal 6xHis-tagged
Form Lyophilized powder
Buffer Lyophilized from a 0.2 μm Filtered 20 mM Tris-HCl, 150 mM NaCl, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
Gene References into Functions
  1. miR-449a suppresses hepatocellular carcinoma tumorigenesis by down-regulating activity in the c-Met/ERK pathway. PMID: 30108016
  2. widely stained in sclerosing stromal tumours of the ovary PMID: 29433373
  3. This novel study, in HIV-positive, preeclampsia, and normotensive pregnancies, demonstrates that HGF was two-fold higher in conducting compared to exchange villi irrespective of the pregnancy type. HIV infection does not influence HGF expression within the conducting and exchange villi. PMID: 29886319
  4. CAFderived HGF promotes angiogenesis. PMID: 29917165
  5. These results suggest that gastric cancer progression is not associated with a unique signaling pathway and that a feedback loop may exist between the HGF/c-Met and Notch1 signaling pathways, which may result in therapeutic resistance. PMID: 29781036
  6. a higher HGF serum level during hemodialysis treatment is associated with a slower loss of residual renal function PMID: 29227979
  7. prolonged treatment of single HGF/c-Met or Hh inhibitor leads to resistance to these single inhibitors, likely because the single c-Met treatment leads to enhanced expression of Shh, and vice versa. Targeting both the HGF/c-Met and Hh pathways simultaneously overcame the resistance to the single-inhibitor treatment and led to a more potent antitumor effect in combination with the chemotherapy treatment. PMID: 28864680
  8. TGF-beta negatively controls the HGF/c-MET pathway by regulating of stemness in glioblastoma. PMID: 29238047
  9. Reduction of fibroblast size upregulates HGF expression, which in turn contributes to loss of collagen, a prominent feature of aged skin. PMID: 28826691
  10. Results of our present study suggest that both IL-6 and HGF derived from Cancer-associated fibroblasts (CAFs)could contribute to the intratumoral androgen metabolism in ER-negative breast carcinoma patients. PMID: 28831645
  11. Hepatocyte growth factor as cardiovascular hormone: role of HGF in the pathogenesis of cardiovascular disease. Review. PMID: 12201209
  12. this study shows that decidual NK cells facilitate the interaction between trophoblastic and endothelial cells via VEGF-C and HGF PMID: 28653669
  13. We identify HGF, acting through the c-Met receptor, as the key polarity-inducing morphogen, which acts to activate b1-integrin-dependent adhesion. HGF and ECM-derived integrin signals co-operate via a c-Src-dependent inhibition of the RhoA-ROCK1 signalling pathway via p190A RhoGAP. PMID: 28888686
  14. High glucose activated Met receptor in HK2 cells independently of HGF, via induction of integrin a5b1 and downstream signaling. This mode of Met activation was associated with tubular cell damage and apoptosis and it may represent a novel pathogenic mechanism and a treatment target in diabetic nephropathy. PMID: 28819999
  15. results show that HGF was involved in regulating Chk1 phosphorylation, and further demonstrate that AKT activity was responsible for this HGF-induced Chk1 phosphorylation. PMID: 28573382
  16. Lymph node metastasis is strongly associated with expression status of HGF and CD133 at the deep invasive front, suggesting the usefulness of these proteins as independent predictive markers of lymph node metastasis in early gastric cancer. PMID: 28595915
  17. EGF-mediated lysosome trafficking, protease secretion, and invasion is regulated by the activity of p38 mitogen activated protein kinase (MAPK) and sodium hydrogen exchangers (NHEs). Interestingly, EGF stimulates anterograde lysosome trafficking through a different mechanism than previously reported for HGF, suggesting that there are redundant signaling pathways that control lysosome positioning PMID: 28978320
  18. HGF/c-MET pathway mediates VEGFR inhibitor resistance and vascular remodeling in NSCLC. PMID: 28559461
  19. We also propose that full-length HGF and HGF-NK1 convey desirable wound healing properties, whilst fibroblasts preferentially expressing more HGF-NK2 readily undergo TGF-beta-driven differentiation into myofibroblasts. PMID: 28837064
  20. In conclusion, these findings indicate that CMs derived from primary culture of NPC fractions of BA liver contain a large amount of active HGF, which may activate hepatic stem/progenitor cells and promote the appearance of hepatocyte-like cells or their clusters through HGF/c-Met signaling. PMID: 28364348
  21. Oxidative stress contributes to HGF-dependent pro-senescence activity of ovarian cancer cells. PMID: 28652056
  22. These results hold promise that dual targeting of HGF and MET by combining extracellular ligand inhibitors with intracellular MET TKIs could be an effective intervention strategy for cancer patients who have acquired resistance that is dependent on total MET protein. PMID: 28341789
  23. cabozantinib suppressed MMP1 expression by blocking HGF-MET signaling and that HGF-MET-MMP1 signaling is involved in the invasiveness and proliferation of BCa cells. These results suggest that cabozantinib might prove useful for future treatment of muscle-invasive BCa. PMID: 28013036
  24. The results suggested that HGF may inhibit TEMT by inhibiting AngII through the JAK2/STAT3 signaling pathway in HK2 cells and HGF may prevent apoptosis induced by AngII. The present study provides a basis for understanding the mechanisms involved in the inhibition of TEMT by HGF, which requires further investigation PMID: 28447719
  25. The identification of novel small-molecule inhibitors of microtubule polymerization highlights the role of the microtubule cytoskeleton in HGF-induced epithelial scattering. PMID: 27245142
  26. miRNA-200a expression was inverse correlation with HGF expression in stromal fibroblasts. High miRNA-200a and low HGF expression in stromal fibroblasts may predict a good prognosis in patients with non-small cell lung cancer. PMID: 27374174
  27. Activation of proHGF by St14 induces mouse embryonic stem cell differentiation. PMID: 27316827
  28. Study shows that Met and HGF have a multi-factorial relationship to the biology and outcome of breast cancer, influenced by gene copy number and protein expression, activation status, stromal environment, and cellular localisation. PMID: 27175600
  29. These results reveal the effect of HGF on the distinct pathways of eosinophil secretory functions and also provide novel insights into the role of HGF in the pathogenesis of allergic inflammation. PMID: 27552115
  30. The hepatocyte growth factor (HGF)-MET receptor tyrosine kinase signaling pathway PMID: 26822708
  31. MiRNA199a-3p suppresses tumor growth, migration, invasion and angiogenesis in hepatocellular carcinoma by targeting VEGFA, VEGFR1, VEGFR2, HGF and MMP2 PMID: 28358369
  32. High HGF expression is associated with castration-resistant progression in androgen dependent metastatic prostate cancer. PMID: 27599544
  33. miR-26a/mir-26b could suppress tumorigenesis and angiogenesis by targeting the HGF-VEGF axis, and could serve as a potential treatment modality for targeted therapy in the clinical treatment of gastric cancer. PMID: 28738343
  34. Which control critical events for colonization such as HGF/Met axis and Wwox, as therapy of bone metastasis. PMID: 28151481
  35. Data suggest that HGF, IL-20, and IL-22 in the serum and bronchoalveolar lavage fluid (BALF) of non-small cell lung cancer (NSCLC) patients before chemotherapy may be a prognostic of cancer progression. PMID: 27573644
  36. Studies indicate that the over-expression of hepatocyte growth factor (HGF) is valuable in colorectal cancer (CRC) prognosis. PMID: 28423584
  37. These results establish HGF/C-Met as a central organizing signal in blood vessel-directed tumor cell migration in vivo and highlight a promising role for C-Met inhibitors in blocking tumor cell streaming and metastasis in vivo, and for use in human trials. PMID: 27893712
  38. Furthermore, activation of HGF/Met signaling increased the expression and transcriptional activity of FOXM1, and the cross talk between FOXM1 and HGF/Met signaling promoted pancreatic ductal adenocarcinoma (PDA)growth and resistance to Met inhibition PMID: 26876216
  39. These data suggest that paxillin appears to influence major cell functions in a diverse range of prostate and breast cancer models. The responsiveness of cells to environmental factors such as HGF or BME may be influenced by paxillin status, although this seems to be dependent on cell type PMID: 28739717
  40. Fibronectin and Hepatocyte Growth Factor were shown to be produced by lung fibroblasts and, furthermore, to enhance malignant pleural mesothelioma cell migration and invasion PMID: 28476806
  41. Glioblastoma patients with high expression of hepatocyte growth factor or unmethylated O(6)-methylguanine-DNA methyltransferase may benefit from onartuzumab plus bevacizumab chemotherapy. PMID: 27918718
  42. Plasma levels of HGF in PAH patients with mild disease were significantly higher than those in healthy controls, suggesting that plasma HGF has potential utility as a diagnostic biomarker for early PAH. PMID: 27342109
  43. Blood HGF was significantly higher in chronic hepatitis C patients with liver fibrosis compared to those without fibrosis. PMID: 27930387
  44. In summary, a decrease in VEGF-A and an increase in sVEGFR1 during chemotherapy and bevacizumab exposure can contribute to both chemotherapy (due to c-MET/b-catenin activation) and bevacizumab (due to low VEGF requirements) resistance. Because HGF levels decrease also during acquired resistance, alternative strategies to HGF-ligand inhibition should be investigated PMID: 28621236
  45. We found the roles of hepatocyte growth factor (HGF) signaling in stria vascularis development for the first time and that lack of HGF signaling in the inner ear leads to profound hearing loss in the mouse. Our findings reveal a novel mechanism that may underlie human deafness DFNB39 and DFNB97. Our findings reveal an additional example of context-dependent c-MET signaling diversity, required here for proper cellular inva PMID: 27488639
  46. Either upregulating miR-182 in retinal pigment epithelial cells or downregulating c-Met expression reduced HGF/SF-induced rises in both retinal pigment epithelial cells proliferation and chemotaxis through declines in Akt activation. PMID: 27936146
  47. Enzyme-linked immunosorbent assay was used to detect the levels of chemokine (C-X-C motif) ligand 12, chemokine (C-X-C motif) ligand 7, hepatocyte growth factor, and fibroblast growth factor 1 in the supernatants of the laryngeal squamous cell carcinoma and control cells. PMID: 28475003
  48. Potential immune markers, including interleukin 1 receptor antagonist, interferon gamma-inducible protein 10, hepatocyte growth factor, soluble p75 tumor necrosis factor alpha receptor, vascular cell adhesion molecule 1, and matrix metalloproteinase 2, were significantly associated with significant plasma leakage during Dengue virus infection. PMID: 28077582
  49. Results indicated that HGF may promote angiogenesis by not only increasing the expression of VEGF but also by decreasing the expression of an angiostatic chemokine, CXCL10 in keratinocytes. PMID: 27718226
  50. overexpression of HGF resulted in resistance to c-MET tyrosine kinase inhibitors through an autocrine manner in gastric cancer cells PMID: 28314274
  51. Activation of the HGF-mediated MET pathway is involved in escape to selective VEGFR inhibition in neuroblastoma suggesting combined inhibition of MET and VEGFR signaling to reduce secondary resistance and enhanced invasiveness. PMID: 27922668
  52. HGF displays an antioxidant response by inducing the glutathione-related protection system. PMID: 27143995
  53. High expression of HGF is associated with invasion in gastric cancer. PMID: 27683052
  54. NK2 and HGF drive motogenic and anti-apoptotic signaling but only HGF drives cell proliferation by activating AKT-pathway signaling. PMID: 27224506
  55. Serum HGF and CK18 levels were significantly up-regulated and positively correlated with metastasis stage, tumor stage, and disease stage of esophageal squamous cell carcinoma. PMID: 27706656
  56. findings indicate that the effects of stimulation with EGF, HGF and TGFbeta on melanoma cell invasiveness could depend on melanoma cell progression stage. PMID: 28076931
  57. activation of HGF/c-MET signaling in an autocrine fashion leads to an aggressive phenotype in SS and targeting of this signaling exerts superior antitumor effects on c-MET-activated SS. HGF/c-MET expression status is a potential biomarker for identification of SS patients with a worse prognosis who can benefit from c-MET inhibitors. PMID: 27779808
  58. HGF efficiently ameliorates the vascular response to stent implantation, and has an important redeeming influence on the deleterious endothelial effects of drug-eluting stents. PMID: 27423724
  59. Plasma HGF is overexpressed in amyloid light-chain cardiac amyloidosis. PMID: 27809600
  60. Using cytokine array analysis, we were able to demonstrate that GSC1 cell growth is mediated through hepatocyte growth factor (HGF)/c-MET signaling pathway which is activated exclusively by HGF secreted from gastric carcinoma mesenchymal stem cells. PMID: 26676563
  61. These data demonstrate that selective perturbation of the satellite cell niche results in distinct sarcoma subtypes in a Pax7 lineage-dependent manner. PMID: 26987019
  62. High Serum level of hepatocyte growth factor is associated with resistance to the treatment with trastuzumab in HER2-positive patients with metastatic gastric cancer PMID: 26716644
  63. Low hemodialysis-induced release of the anti-inflammatory cytokine HGF correlates with impaired quality of life in that group of patients PMID: 26722560
  64. Treatment of bronchiolitis Obliterans with HGF gene modified MSCs results in reduction of local inflammation and promotion in recovery of allograft trachea histopathology. PMID: 26857230
  65. Case Report: favorable outcome in hepatitis A complicated with acute renal failure and high hepatocyte growth factor. PMID: 26327776
  66. Report the application of advanced quantitative fluorescence microscopy techniques to study changes in the oligomerization state of the RTK Met in response to stimulation by its endogenous ligand hepatocyte growth factor. PMID: 27094128
  67. The findings suggest that the oncogenic activity of hepsin arises not only from elevated expression level but also from depletion of HAI-1, events which together trigger gain-of-function activity impacting HGF/MET signalling and epithelial cohesion PMID: 26165838
  68. we suggest that combinatorial therapeutic strategies with HGF and c-Met inhibitors comprise promising candidates for overcoming sorafenib resistance. PMID: 26790028
  69. The findings support a role for HGF in regulating both myoblast cell number and terminal differentiation. PMID: 26135603
  70. Pre-osteoblasts stimulate migration of breast cancer cells via the HGF/MET pathway. PMID: 26934743
  71. Peptide HGP-1 could disrupt the activation of HGF/MET signaling to influence tumor cell activity. PMID: 26254225
  72. The signal transduction mechanisms in a gene interaction network describing hepatocyte growth factor-stimulated cell migration and proliferation from gene-expression measured with microarray PMID: 26518250
  73. Adenoviral delivery of truncated MMP-8 fused with the hepatocyte growth factor mutant 1K1 ameliorates liver cirrhosis and promotes hepatocyte proliferation. PMID: 26527860
  74. Treatment of silicosis with HGF-expressed autologous bone marrow stromal cells has been reported. PMID: 26400297
  75. High serum HGF levels in breast cancer patients are associated with clinically detectable regional node metastasis and, paradoxically, with longer relapse-free survival in stage II/III breast cancer. PMID: 26577828
  76. level of HGF is associated with incident diabetes and may reflect a unique type of impaired metabolism PMID: 26892517
  77. reduced serum level in kidney stone patients, relative to healthy subjects; no difference in allele frequency PMID: 26081218
  78. study suggests that GPC3 is involved in HCC cell migration and motility through HS chain-mediated cooperation with the HGF/Met pathway, showing how HS targeting has potential therapeutic implications for liver cancer PMID: 26332121
  79. these data demonstrate that FGF-2 priming enhances the angiogenic potential of SHED through the secretion of both HGF and VEGF. PMID: 26798059
  80. HGF and its receptor, cMET, are antagonized in human lung cancer cells treated with YangZheng XiaoJi PMID: 26310485
  81. HGF upregulates the expression of DNA methyltransferase 1 (DNMT1) in HEPG2 cells. PMID: 26099202
  82. Evidence for association was found for both of the tested loci.It was strongest for rs3735520:G>A near HGF with A allele being a risk factor and rs2956540:G>C within LOX with C allele having a protective effect PMID: 25735481
  83. Early treatment with HGF/IgG complexes after myocardial ischaemia with reperfusion may rescue tissue through vasoprotection conferred by c-Met and RYK signalling PMID: 26025956
  84. integrin alpha6beta4 stimulates invasion by promoting autocrine EGFR signaling through transcriptional up-regulation of key EGFR family members and by facilitating HGF-stimulated EGFR ligand secretion PMID: 26381405
  85. Direct binding of CD44v6 to HGF and VEGF was demonstrated in pull-down assays PMID: 26181364
  86. hepatocyte growth factor has a role in differentiation potential of bone marrow-derived mesenchymal stem cells treated with L-ascorbic acid 2-phosphate and fibroblast growth factor-2 PMID: 25714612
  87. Results show that MET and HGF overexpression were significantly correlated in undifferentiated pleomorphic sarcomas or angiosarcomas suggesting an autocrine activation. These proteins may be potential biomarkers fro these types of sarcoma. PMID: 25844809
  88. Hepatocyte Growth Factor Gene-Modified Mesenchymal Stem Cells Augment Sinonasal Wound Healing. PMID: 25835956
  89. our work reveals a critical relationship of Cox-2/PGE2 and HGF/MET signaling in promoting glioma cells proliferation PMID: 26097553
  90. In donors significantly lower levels of IL-6, TNF-alpha, HGF, and TGF-beta were detected in portal blood compared to hepatic vein In patients with cirrhosis there were no significant differences of IL-6, TNF-alpha, and TGF-beta levels between portal and hepatic veins PMID: 26090463
  91. These results suggested that the expression of the HGF/cMet pathway in gastric cancer may be a potential predictive factor for disease progression. PMID: 25592281
  92. Sequential and timely transfection of hepatocyte growth factor and monocyte chemotactic protein-1 ameliorates hyperkinetic pulmonary artery hypertension in rabbits. PMID: 25940417
  93. HGF induction is a crucial contributor to the acceleration of cell dedifferentiation and growth. PMID: 25793303
  94. HGF secreted by ovarian cancer cells induces the mesothelial-to-mesenchymal transition and stimulates the invasion of mesothelial cells. HGF secreted by ovarian cancer cells plays an important role in cancer peritoneal implantation. PMID: 26335595
  95. HGF level may be a new detection method used for the rapid diagnosis of benign and malignant SPNs. PMID: 25973105
  96. HGF/cMET signaling plays and important role in adrenocortical carcinoma growth and resistance to commonly used treatments. PMID: 26282167
  97. HGF and TGFbeta1 of bone-metastasis microenvironment acted co-ordinately, influencing non redundant pathways regulated by Twist program or Snail-transcription factor, with reversible MET switch PMID: 26041563
  98. Genetic variation contributes to the interindividual variation in growth factor levels and explains a modest proportion of circulating hepatocyte growth factor, Ang-2, and Tie-2. PMID: 25552591
  99. High HGF (and follistatin) expression in tumor tissue from esophageal cancer patients was associated with poor prognosis. PMID: 25885021
  100. Data show that Ash1l and EZH2 methyltransferases protein expression was significantly elevated in fibrotic liver and decreased in mutant matrix metalloproteinase-9 (mMMP-9) and human hepatocyte growth factor (hHGF) gene transfer treated fibrotic livers. PMID: 25380300

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Involvement in disease Deafness, autosomal recessive, 39 (DFNB39)
Protein Families Peptidase S1 family, Plasminogen subfamily
Database Links

HGNC: 4893

OMIM: 142409

KEGG: hsa:3082

STRING: 9606.ENSP00000222390

UniGene: Hs.396530

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