Human insulin-like growth factors 2,IGF-2 ELISA Kit

Code CSB-E04583h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01344
Lead Time 3-5 working days
Protein Biological Process 2 glyconeogenesis and glycometabolism
Abbreviation IGF2
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name insulin-like growth factor 2 (somatomedin A)
Alias C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974, OTTHUMP00000011018|OTTHUMP00000011157|insulin-like growth factor 2|insulin-like growth factor II|insulin-like growth factor type 2|putative insulin-like
Species Homo sapiens (Human)
Protein Biological Process 3 Carbohydrate metabolism
Sample Types serum, plasma, cell culture supernates, urine, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3 region and to the upstream INS gene at the 5 region. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene.
HGNC 5466
RGD 2870
MGI 96434
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 84  
Range % 80-92  
1:400 Average % 97  
Range % 91-105  
1:800 Average % 97  
Range % 92-104  
1:1600 Average % 93  
Range % 86-98  
The recovery of human IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-104  
EDTA plasma (n=4) 97 90-106  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.789 2.769 2.779 2.673  
2000 2.034 2.046 2.040 1.934  
1000 1.518 1.478 1.498 1.392  
500 1.205 1.206 1.206 1.100  
250 0.823 0.839 0.831 0.725  
125 0.536 0.546 0.541 0.435  
62.5 0.349 0.369 0.359 0.253  
0 0.102 0.110 0.106    
Still Have Questions? Leave a Message or Start an on-line Chat
Function The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF-II is influenced by placental lactogen. Also involved in tissue differentiation. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (By similarity). In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable).
Involvement in disease Silver-Russell syndrome (SRS); Growth restriction, severe, with distinctive facies (GRDF)
Subcellular Location Secreted
Protein Families Insulin family
Tissue Specificity Expressed in heart, placenta, lung, liver, muscle, kidney, tongue, limb, eye and pancreas.
Database Links

HGNC: 5466

OMIM: 147470

KEGG: hsa:3481

STRING: 9606.ENSP00000391826

UniGene: Hs.272259

Pathway MAPK signaling pathway
PI3K-Akt signaling pathway
Ras signaling pathway

Related Products

IGF2 Antibodies

IGF2 Antibodies for Human

IGF2 Antibodies for Homo sapiens (Human)

IGF2 Proteins

IGF2 Proteins for Homo sapiens (Human)

Most popular with customers


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 CUSABIO TECHNOLOGY LLC All Rights Reserved.