Human insulin-like growth factors 2,IGF-2 ELISA Kit

Human insulin-like growth factors 2,IGF-2 ELISA Kit

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IGF2 Antibodies

IGF2 Proteins

IGF2 Antibodies

IGF2 Proteins

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Human insulin-like growth factors 2,IGF-2 ELISA Kit

Specification
Target

Code

CSB-E04583h

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P01344

Lead Time

3-5 working days

Protein Biological Process 2

glyconeogenesis and glycometabolism

Abbreviation

IGF2

Protein Biological Process 1

Biosynthesis/Metabolism

Target Name

insulin-like growth factor 2 (somatomedin A)

Alias

Species

Homo sapiens (Human)

Protein Biological Process 3

Carbohydrate metabolism

Sample Types

serum, plasma, cell culture supernates, urine, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3 region and to the upstream INS gene at the 5 region. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene.

HGNC

5466

RGD

2870

MGI

96434

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	84
	Range %	80-92
1:400	Average %	97
	Range %	91-105
1:800	Average %	97
	Range %	92-104
1:1600	Average %	93
	Range %	86-98

Recovery

The recovery of human IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-104
EDTA plasma (n=4)	97	90-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.789	2.769	2.779	2.673
2000	2.034	2.046	2.040	1.934
1000	1.518	1.478	1.498	1.392
500	1.205	1.206	1.206	1.100
250	0.823	0.839	0.831	0.725
125	0.536	0.546	0.541	0.435
62.5	0.349	0.369	0.359	0.253
0	0.102	0.110	0.106

References

Prenatal ambient air pollution and cord blood insulin-like growth factor (IGF) in the ENVIR ON AGE birth cohort Saskia Put.et al,Universiteit Hasselt ,2017
IGF2 mRNA binding protein-2 is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1 Dai N.et al,Elife,2017
Prenatal ambient air pollution and cord blood insulin-like growth factor (IGF) in the ENVIRONAGE birth cohort Saskia .et al,Universiteit Hasselt ,2017
Inhibition of autocrine IGF-II on effect of human HepG2 cell proliferation and angiogenesis factor expression Yao N et al,Tumour Biol,2012
Characteristics of hepatic igf-ii expression and monitored levels of circulating igf-ii mRNA in metastasis of hepatocellular carcinoma Qian J et al,American Journal of Clinical Pathology,2010

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Function	The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF-II is influenced by placental lactogen. Also involved in tissue differentiation. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (By similarity). In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable).
Involvement in disease	Silver-Russell syndrome (SRS); Growth restriction, severe, with distinctive facies (GRDF)
Subcellular Location	Secreted
Protein Families	Insulin family
Tissue Specificity	Expressed in heart, placenta, lung, liver, muscle, kidney, tongue, limb, eye and pancreas.
Database Links	HGNC: 5466 OMIM: 147470 KEGG: hsa:3481 STRING: 9606.ENSP00000391826 UniGene: Hs.272259
Pathway	MAPK signaling pathway PI3K-Akt signaling pathway Ras signaling pathway

IGF2 Antibody

IGF2 Protein

IGF2 Antibodies for Human

Code	Product Name	Species Reactivity	Application
CSB-RA011088A0HU	IGF2 Antibody	Human	ELISA

IGF2 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA011088KA01HU	IGF2 Antibody	Human,Mouse	ELISA,WB

IGF2 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-AP003891HU	Recombinant Human Insulin-like growth factor II(IGF2) (Active)	E.coli
CSB-YP011088HU CSB-EP011088HU CSB-BP011088HU CSB-MP011088HU	Recombinant Human Insulin-like growth factor II(IGF2)	Yeast E.coli Baculovirus Mammalian cell
CSB-AP002551HU	Recombinant Human Insulin-like growth factor II protein(IGF2) (Active)	E.coli
CSB-RP083844h	Recombinant Human Insulin-like growth factor II protein(PP1446),partial	E.coli

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.