Recombinant Human Insulin-like growth factor II protein(IGF2) (Active)

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Code CSB-AP002551HU
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Product Details

Purity >98% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of >5.0x105 IU/mg.
Target Names IGF2
Uniprot No. P01344
Research Area Cancer
Alternative Names C11orf43; IGF 2; IGF II; IGF-II; IGF2; IGF2_HUMAN; IGFII; INSIGF; insulin like growth factor 2 (somatomedin A) ; Insulin like Growth Factor 2; Insulin like growth factor II; Insulin like growth factor II precursor; Insulin like growth factor type 2; pp9974; Preptin; putative insulin like growth factor II associated protein; Somatomedin A; Somatomedin-A
Species Homo sapiens (Human)
Source E.coli
Expression Region 25-91aa
Mol. Weight 7.5 kDa
Protein Length Partial
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 0.02 % Tween-20
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF-II is influenced by placental lactogen. Also involved in tissue differentiation. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (By similarity). In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable).
Gene References into Functions
  1. serum preptin levels in women decrease after menopause and have a positive correlation with estradiol, femoral and total hip bone mineral density. PMID: 29134283
  2. Analysis of patient serum samples showed that concurrent elevation of insulin like growth factor 2 (IGF2) and vascular endothelial growth factor (VEGF) levels may serve as a prognostic biomarker for oesophageal cancer. PMID: 28186102
  3. Our results revealed that IGF-II promotes cell proliferation and EMT in HCC cells. PMID: 29970663
  4. Serum preptin levels were significantly higher in women with polycystic ovary syndrome compared with controls. PMID: 29374985
  5. infants with intrauterine growth restriction had higher serum levels of IGF2 if they had the A/G genotype at the ApaI restriction fragment length polymorphism and higher values of IGF2R if they had the A/A genotype PMID: 28460554
  6. Upregulation of IGF-II expression is associated with ovarian cancer. PMID: 28829218
  7. Data suggest that high IGF2 DMR methylation status would be a phenomenon that is observed with the progression of gastric cancer (GC) toward more aggressive features, supporting their potential utility as a biomarker in GC patients. PMID: 28871451
  8. Data suggest that expression of CDKN1C and IGF2 is significantly up-regulated in placenta after assisted reproductive technology; DNA methylation was significantly down-regulated in DMR of CDKN1C and up-regulated in DMR of IGF2. (CDKN1C = cyclin-dependent kinase inhibitor-1C; IGF2 = insulin like growth factor 2; DMR = differential methylation regions) PMID: 29277274
  9. The expression of miR-3941 was significantly down-regulated in acute pneumonia; IGF2 was confirmed as a direct target gene of miR-3941. PMID: 29328418
  10. Our data reveal that vigilin is essential for maintenance of imprinting of IGF2 gene via functional interaction between KH1-7 domains of vigilin and zinc-finger domains of CTCF. PMID: 29157910
  11. Low IGF-II serum levels were associated with Pancreatic Cancer. PMID: 28681154
  12. Rapamycin-independent IGF2 expression in Tsc2-null mouse embryo fibroblasts and human lymphangioleiomyomatosis cells. PMID: 29758070
  13. IGF2 rs680 polymorphism may have a role in endurance among Israeli athletes PMID: 29107196
  14. low methylation of the Igf2 gene promoter region may promote the expression of Igf2 and miR4835p; this, in turn, induces the degradation of miR4835p target genes, and leads to the upregulation of oncogenes and the downregulation of tumor suppressors, which promotes the development of ESCC. PMID: 29207103
  15. Results found IGF2 as a direct target gene of miR615 and restoring its expression reverses the inhibitory effects of miR615 in human esophageal squamous cell carcinoma cell motility. PMID: 29115555
  16. A common indel variant in the 3'UTR of the IGF2 gene was associated with the risk of impaired renal function in an elderly population. PMID: 29889555
  17. High molecular weight IGF-2 was associated with hypoglycemia in Recurrent Renal Cell Carcinoma. PMID: 24711554
  18. Impairment of IGF2 gene expression in prostate cancer is triggered by epigenetic dysregulation of IGF2-DMR0 and its interaction with KLF4 PMID: 29017567
  19. HMGA1P7 mRNA sustains the H19 and Igf2 overexpression by acting as miRNA decoy. PMID: 27874091
  20. Data suggest that blocking insulin-like growth factor 2 (IGF2) is a potential therapeutic mechanism for hepatocellular carcinoma (HCC). PMID: 29413895
  21. IGF2 expression varies among mesenchymal stem cells derived from amniotic fluid, amnion, endometrium, and Wharton's jelly during culture period. PMID: 28629288
  22. Results showed that IGF-2 was persistently expressed in oculomotor neurons in health and amyotrophic lateral sclerosis (ALS) and thus could play a role in oculomotor resistance in this disease. PMID: 27180807
  23. Studies indicate that miR-663b is epigenetically repressed by long non-coding RNA HOTAIR and exerts its tumor-suppressive function via targeting insulin-like growth factor 2 (IGF2) in pancreatic cancer. PMID: 27895308
  24. IGF-II-mediated loss of E-cadherin is central in developing hepatomegaly in mice and abnormal cell growth in the hepatoma cell line PMID: 27486970
  25. Aberrant IGF2 imprinting may be an intrinsic epigenetic control mechanism that enhances stemness, self-renewal and chemo/radiotherapy resistance in cancer stem cells. PMID: 27275535
  26. DNMT1-mediated transcriptional upregulation of IGF2 is a novel mechanism of resistance to HDIs, highlighting the role of epigenetic deregulation of IGF2 in HDI resistance and the potential value of the H19/IGF2 ICR hypermethylation and DNMT1 expression as predictive biomarkers in HDI-based anticancer therapies. PMID: 27582487
  27. Human H19/Igf2 imprinting control regions can functionally replace mouse H19/Igf2 imprinting control regions on the maternal allele. PMID: 27621468
  28. IGF2 may exert its oncofunction, at least partly, through its parasitic miR-483 which suppressed DLC-1 in colorectal cancer cells. PMID: 27366946
  29. We report here the first deletions of ICR1 associated with hypomethylation of the IGF2/H19 domain leading to SRS. PMID: 27701793
  30. Methylation patterns of IGF2 regulatory regions can discriminate adrenocortical carcinomas from adrenocortical adenomas with high diagnostic accuracy. PMID: 27535174
  31. IGF-II siRNA inactivates the FAK/PI3K/Akt signaling pathway, and further reduces cell proliferation, N-ras and C-myc levels in SMMC-7721 cells. PMID: 27768959
  32. Study shows impact of folic acid intake during pregnancy on genomic imprinting of IGF2/H19 and 1-carbon metabolism PMID: 28778973
  33. produced by pericentral hepatocytes to promote hepatocyte proliferation and repair tissue damage in the setting of chronic liver injury PMID: 28653763
  34. These findings suggest that reducing IGF2 isoform 2 expression in relevant tissues has potential as a new therapeutic strategy for type 2 diabetes, even beyond the Latin American population, with no major adverse effects on health or reproduction. PMID: 28838971
  35. this is the first report of IGF2/H19 domain triplication associated with BWS or SRS and the second report of a maternal amplification of this region in a patient with clinical features of SRS. This study further supports the hypotheses that the amplification of the H19/IGF2 region on the maternal chromosome can lead to an SRS phenotype. PMID: 27612309
  36. stroma-induced IGF2 promotes colon cancer progression in a paracrine and autocrine manner and propose IGF2 as potential target for tumor stroma cotargeting strategies. PMID: 28534511
  37. We identified vascular INSR expression as a potential biomarker for progression in bladder cancer. The data suggest that IGF-2/INSR mediated paracrine crosstalk between bladder cancer cells and endothelial cells is functionally involved in tumour angiogenesis and may thus represent a new therapeutic target. PMID: 28295307
  38. Methylation of IGF2AS is altered 20 years after preterm birth at VLBW. Altered methylation may be a mechanism of later increased disease risk but more data are needed to indicate causality PMID: 23840686
  39. Prenatal 'unhealthy diet' was positively associated with IGF2 methylation at birth, and with ADHD and conduct disorder. PMID: 27535767
  40. High IGF2 expression is associated with breast cancer. PMID: 27546618
  41. IGF2 and insulin receptor A are important for uterine leiomyoma stem cell proliferation and may represent paracrine signaling between leiomyoma cell types. PMID: 28324020
  42. IGF2 overexpression is associated with cancer. PMID: 27869826
  43. Long non-coding RNA 91H is overexpressed in breast cancer and 91H-induced epigenetic modifications on H19/IGF2 locus suggest that 91H may play essential role in breast cancer development. PMID: 27780718
  44. our results suggest that IGF2 via AKT1 contributes to non-canonical wnt signaling. PMID: 26984550
  45. Elevated insulin-like growth factor 2 expression is associated with Beckwith-Wiedemann syndrome. PMID: 27650505
  46. Here we present IGF2, which is re-expressed through epigenetic mechanisms, as the first actionable validated epi-driver in hepatocellular carcinoma. PMID: 27614046
  47. results demonstrate that the H19-Igf2 axis is negatively regulated by CTCF-PHB1 cooperation and that H19 is involved in modulating the growth-suppressive effect of PHB1 in the liver. PMID: 27687727
  48. Compared with obstructive azoospermia (OA) as normal control, our results suggest that miR-210 was significantly up-regulated in testis of patients with non-obstructive azoospermia (P<0.05), and IGF2 was down-regulated, but without a significant difference. PMID: 27535712
  49. The identification of the INS-IGF2 read-through transcript specifically in tumor tissue but not in normal pancreatic tissue suggests that high expression of INS-IGF2 could be neoplasiaspecific PMID: 27667266
  50. DNA methylation of imprinted loci of IGF2 is not affected in Parkinson's disease patients. PMID: 28081695
  51. direct autocrine/paracrine tumor cell activation through IGF2 and shows an association between IGF2 deregulation and cellular proliferation, adhesion and, to a limited extent, apoptosis. PMID: 27337954
  52. Maternal anxiety in pregnancy is associated with decreased IGF2/H19 imprinting control region DNA methylation in progeny at birth, particularly in female, low birth weight neonates. PMID: 27023171
  53. Molar tissues showed significant differences of allelic distribution of IGF2 and H19 from normal placenta tissues. PMID: 27797252
  54. IGF-II expression was suppressed, suggesting that this system is one of the mechanisms underlying effects of ID1 in salivary gland cancer cells PMID: 27466488
  55. Findings indicate that the IGF2 ApaI polymorphism, but not the ACTN3 R577X polymorphism, may be a candidate gene associated with some types of muscle strength and judo status. PMID: 26677828
  56. DNA methylation in imprinted genes IGF2 and GNASXL is associated with prenatal maternal stress PMID: 26333472
  57. These results demonstrate that IGF2 mRNA expression is more upregulated in fibroadenomas (FAs) and phyllodes tumors (PTs) than in normal tissues PMID: 26676988
  58. Ox-LDL induces NF-kappaB activation and IL-6 expression via IGF2 activation in macrophages. PMID: 26063187
  59. suggest that molecular subtypes of the LIN-28B/let-7a/IGF-II axis associate with heterogeneous progression PMID: 26751131
  60. There was a positive correlation between the IGF2 levels of and those of inflammatory mediators in HIV Infections. PMID: 25890304
  61. Therefore, our results suggest that IGF-II can promote cartilage integrity and halt knee joint destruction in Osteoarthritis PMID: 26015264
  62. cord blood levels significantly reduced in intrauterine growth restriction PMID: 26118397
  63. We conclude that recombinant adenovirus-mediated siRNA targeting CD147 based on the IGF2 LOI system inhibited the growth of the LOI cells in vitro and in vivo, which would provide a novel approach for targeted CRC gene therapy. PMID: 26397886
  64. Differential IGF2 and H19 expression characterize pheochromocytomas and adrenocortical carcinomas. Aberrant DNA methylation is found throughout the IGF2/H19 locus. Somatic copy number changes of chr11p15.5 are abundant and correlate with overexpression. PMID: 26400872
  65. the evidence of strong associations between methylation of insulin-like growth factor 2/H19 and macrosomia induced by intrauterine hyperglycemia. PMID: 26840070
  66. IGF2 expression in infantile hemangioma is strongly related to the expression of a cancer testes and suspected oncogene BORIS. PMID: 26496499
  67. We propose that these interactions occur at the IGF2 and H19 gene cluster and are involved in the formation of loops between the IGF2 and H19 promoters and the enhancer, and thus the expression of the corresponding genes PMID: 26116569
  68. GF2 gene may be aging-related gene and involved in Osteoporosis. PMID: 25771989
  69. Hypomethylation of the differently methylated imprinting center region 1 between the IGF2 and H19 loci on chromosome 11p15 is associated with Russell-Silver syndrome. PMID: 25639378
  70. Data provide evidence that IGF2 expression is frequently increased in ovarian cancer tissues, and high expression of IGF2 may be a significant prognostic factor for poor survival in ovarian cancer patients. PMID: 26063585
  71. Data indicate insulin like growth factor 2 (IGF2) as one of the top upregulated transcripts before and during early in vitro osteogenic differentiation. PMID: 25931278
  72. We hypothesised that the associations between increased maternal distress symptoms and changes in placental gene expression including IGF2 and genes regulating fetal glucocorticoid exposure are more pronounced in SO pregnancy. PMID: 26056743
  73. Positive expression of IGF2 in spinal giant cell tumor was associated with increased microvessel density and expression of IMP3. PMID: 25740666
  74. Serum IGF2 was increased in hepatocellular carcinoma patients compared to patients with liver cirrhosis, but lower than in healthy controls. PMID: 26068014
  75. High IGF2 expression is associated with Hepatocellular Carcinoma. PMID: 25987019
  76. IGF2 on chromosome 11p is overexpressed in 100% of the examined paediatric adrenocortical tumours. PMID: 25743702
  77. SUZ12 is a key molecule in the regulation of monoallelic expression of IGF2. PMID: 26407907
  78. IGF-II was associated with birth weight and expressed at high levels, which suggests that IGF-II may continue to play an important role after birth. H19 DMR methylation may be involved in controlling IGF-II expression PMID: 25503665
  79. Imp2 regulates the activity of IGF2, which further activates PI3K/Akt signaling. PMID: 25719943
  80. The IGF2 gene was variably methylated in monozygotic twins discordant for depressive disorder. PMID: 25918994
  81. Biologic roles of estrogen receptor-beta and insulin-like growth factor-2 in triple-negative breast cancer. PMID: 25874233
  82. fluid shear stress induces the synthesis of Insulin growth factor-2 and vascular endothelial growth factor (VEGF) B and D, which in turn transactivate MMP-12. PMID: 25435370
  83. There is a positive loop interaction between FSH and IGF-2 that is critical for human granulosa cell proliferation and differentiation. PMID: 26066673
  84. Overexpression of IGF2 led to beta-cell dedifferentiation and endoplasmic reticulum stress causing islet dysfunction. PMID: 25971976
  85. High IGF2 mRNA expression is significantly associated with ovarian cancer progression. PMID: 24932685
  86. High Insulin-like Growth Factor 2 Gene Expression is associated with Embryonal Rhabdomyosarcoma. PMID: 26056800
  87. Both flanks of the IGF-II C domain play important roles in the greater ability of IGF-II to bind and activate IR receptors than IGF-I. PMID: 25458127
  88. Expression of IGF2 in several HCC cell lines was lower than in normal cell lines PMID: 25556430
  89. IGF2 variant (c.191C-->A, p.Ser64Ter) with evidence of pathogenicity in a family with four members who have growth restriction. Dysmorphic features are consistent with a role of deficient IGF-II levels in the cause of the Silver-Russell syndrome. PMID: 26154720
  90. upregulation of IGF2/Igf2 is likely controlled by hypermethylation of H19 DMR1 in human NTDs, however, in acute external RA-induced NTD mice it is potentially determined by more open chromatin structure PMID: 25423083
  91. Exhaustive methylation analysis revealed uneven profiles of methylation at IGF2/ICR1/H19 11p15 loci in Russell Silver syndrome. PMID: 25395389
  92. IGFII and N33 methylation status may be related to gastric carcinogenesis. PMID: 25086101
  93. Data indicate that methylation levels at insulin-like growth factor 2 (IGF2)/H19 long non-coding RNA (H19) imprinted regions were associated with maternal body mass index (BMI). PMID: 25293351
  94. As in Dupuytren's disease, beta-catenin levels and IGF2 expression are elevated in Frozen Shoulder Syndrome. PMID: 25090267
  95. higher baseline IGF-II and IGFBP-2 predict increased HDL concentration over time, implicating IGF-II in modulation of circulating HDL-cholesterol concentrations. PMID: 24081183
  96. Loss of imprinting of insulin-like growth factor 2 is associated with increased risk of primary lung cancer. PMID: 25292066
  97. High IGF2 is associated with esophageal squamous cell carcinoma. PMID: 24706416
  98. IGF-II, TGF-beta1 and VEGF-A and its receptor in malignant tumor tissue, as well as increasedplasmin release from proenzyme and MMP-3 activationis apparently associated with the formation of pathogenic mechanism of vasculature development PMID: 25993872
  99. The gene expression pattern of CDKN1C, H19, IGF2, KCNQ1 and PHLDA2 genes was evaluated using RT-PCR. PMID: 24986528
  100. Report association between histopathological findings and IGF2 differentially methylated region hypomethylation in serrated colorectal neoplasms. PMID: 25110432

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Involvement in disease Silver-Russell syndrome (SRS); Growth restriction, severe, with distinctive facies (GRDF)
Subcellular Location Secreted
Protein Families Insulin family
Tissue Specificity Expressed in heart, placenta, lung, liver, muscle, kidney, tongue, limb, eye and pancreas.
Database Links

HGNC: 5466

OMIM: 147470

KEGG: hsa:3481

STRING: 9606.ENSP00000391826

UniGene: Hs.272259

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