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| Code | CSB-E12637h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human MFGE8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 85-100 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 80-93 | |||||
| The recovery of human MFGE8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-99 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human milk fat globule-EGF factor 8 protein (MFGE8) Elisa kit is suitable for the quantitative measurement of human MFGE8 in serum, cell culture supernates, urine, or cerebrospinal fluid (CSF). This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of MFGE8 in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
MFGE8 is a multifunctional glycoprotein that is found in lacteal glands and milk fat globules and contributes to connecting αvβ3 integrin on phagocytic macrophages with phosphatidylserine on apoptotic cells thus enhancing engulfment of apoptotic cells. This process is essential for maintaining the host immune system under physiological conditions. In addition, MFGE8 also directly regulates various cellular functions, such as attenuating inflammation and healing of injured tissues. MFGE8 has been demonstrated to have anti-inflammatory and regenerating roles during intestinal inflammation. Studies have recently identified MFGE8 as a novel and outstanding modulator for vascular aging via targeting endothelial cells (ECs) and vascular smooth muscle cells (VSMCs).
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