Thrombospondin-1 is a large matricellular glycoprotein that plays important roles in cell adhesion, migration, and angiogenesis regulation. This multifunctional protein is secreted by various cell types including platelets, endothelial cells, and smooth muscle cells, where it modulates extracellular matrix interactions and influences vascular remodeling processes. THBS1 shows potent anti-angiogenic properties by inhibiting endothelial cell proliferation and migration, making it an important regulator of tumor growth and wound healing. Its expression levels are altered in numerous pathological conditions, including cardiovascular disease, cancer, and inflammatory disorders. This positions thrombospondin-1 as a significant biomarker and therapeutic target in biomedical research.
The Human thrombospondin 1,TSP-1 ELISA Kit (CSB-E08763h) uses a quantitative sandwich measurement principle to detect THBS1 in multiple sample types including serum, plasma, breast milk, saliva, tissue homogenates, and cell culture supernatants. This assay provides a detection range of 2.7 ng/ml to 2000 ng/ml with a sensitivity of 2.7 ng/ml. The protocol requires 50-100 μl sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research investigating thrombotic and hemostatic disorders, where thrombospondin-1 serves as a key biomarker alongside other coagulation-related proteins. Studies have used the kit to measure plasma thrombospondin-1 levels in clinical samples, supporting research into vascular pathophysiology and blood coagulation mechanisms.
• Hemostatic research: Quantification of thrombospondin-1 in plasma samples alongside other coagulation factors to study thrombotic disorders and vascular dysfunction
• Clinical biomarker studies: Measurement of plasma thrombospondin-1 levels in patient samples collected from routine clinical testing to assess disease-related changes
• Vascular pathophysiology: Study of thrombospondin-1 as part of multi-analyte panels examining the interplay between coagulation proteins and endothelial function
• Growth factor profiling: Analysis of thrombospondin-1 levels as part of broader growth factor characterization studies
