Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

THBS1

Uniprot No.

P07996

Research Area

Cell Adhesion

Alternative Names

THBS 1; THBS; Thbs1; Thrombospondin 1; Thrombospondin-1; TSP 1; TSP; TSP1; TSP1_HUMAN

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

19-344aa

Target Protein Sequence

NRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

40.6kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The production of this Recombinant Human THBS1 protein started with the THBS1 gene synthesis. And then using recombinant DNA technology, the THBS1 gene was inserted into an expression vector so that we could get the recombinant express plasmid of THBS1. Transform the plasmid into the cells of E.coli, culture the cells and we could get the desired Recombinant Human THBS1 protein. But the work was not completed, protein purification and a strict QC system were performed in the last step. The purity is 90%+ determined by SDS-PAGE.

THBS1 (also named TSP or TSP1) is a gene providing an instruction of making a protein named thrombospondin-1 (abbreviated as THBS1) in human. THBS1 protein is also called glycoprotein G and is a subunit of a disulfide-linked homotrimeric protein. This protein is well known as a natural angiogenesis inhibitor and has a potent effect on tumor growth of the ability to inhibit angiogenesis. The anti-angiogenic effect of thrombospondin-1 is mediated by inhibiting vascular endothelial cell adhesion and migration.

Target Background

Function

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.

Gene References into Functions

High THBS1 expression is associated with migration, invasion, and progression of bladder cancer. PMID: 29602637
intracellular dynamics of the TSP1-induced apoptosis signaling pathway PMID: 29258506
TSP-1 effectively elevated P. gingivalis LPS-induced inflammation mediated by the NF-kappaB pathway and may be critical for pathology of periodontitis. PMID: 28634844
These findings indicate that PRR13/THBS1 and TXN expression could be used for the prediction of resistance to treatment of epithelial ovarian cancer patients. PMID: 27779244
TSP-1 appears to play an accessory role in modulating Mp activity in humans and BlaJ mice in a gender, age and muscle-dependent manner, but is unlikely a primary driver of disease progression of dysferlinopathy. PMID: 29206970
TSP-4 A387P polymorphism, but not TSP-1 polymorphism, is an independent risk factor for acute myocardial infarction in Egyptians. PMID: 29336258
Results show that the thrombospondin 1 (TSP1) and its receptor CD47 (CD47) axis selectively regulates NADPH oxidase 1 (Nox1) in the regulation of endothelial senescence and suggest potential targets for controlling the aging process at the molecular level. PMID: 29042481
15-LOX-1 expression in colon and prostate cancer cells leads to reduced angiogenesis. These changes could be mediated by an increase in the expression of both ICAM-1 and the anti-angiogenic protein TSP-1. PMID: 28757355
miR-98 can suppress the expression of TSP1 in the peripheral B cells of patients with allergic asthma. PMID: 28760845
Data indicate correlation between the levels of thrombospondin-1 and overall survival of ovarian cancer patients, suggesting thrombospondin-1 may be used as a prognostic factor in ovarian cancer patients. PMID: 28655131
Studies show that thrombospondin-1 (Thbs-1), is a prolific contributor to the production and modulation of ROS in large conductance vessels and in the peripheral circulation. Recently, the presence of physiologically relevant circulating Thbs-1 levels was proven to also disrupt vasodilation to nitric oxide (NO) in coronary arterioles from aged animals, negatively impacting coronary blood flow reserve. [review] PMID: 28762749
Our findings suggest that Rab37-mediated TSP1 secretion in cancer cells suppresses metastasis and angiogenesis via a cross-talk with endothelial cells and reveal a novel component of the vesicular exocytic machinery in tumor microenvironment and tumor progression PMID: 28151721
TSP-1 strongly potentiated the proliferative and migratory activity of PDGF on mesenchymal stromal cells. PMID: 26992552
This study demonstrated that THBS! was detected in the serums of Alzheimer disease patients. PMID: 27911324
Functional studies showed THBS1 and TNC to mediate chemoresistance through the integrin beta1/mTOR pathway. PMID: 27487140
These findings shed light on the mechanisms leading to beta-cell failure during metabolic stress and point to THBS1 as an interesting therapeutic target to prevent oxidative stress in type 2 diabetes. PMID: 27588705
Thrombospondin-1 gene polymorphism is associated with estimated pulmonary artery pressure in patients with sickle cell anemia. PMID: 28033687
High plasma levels of TSP-1 are associated with increased pulmonary arterial pressure, increased pulmonary vascular resistance and decreased survival pulmonary hypertension. PMID: 27473366
Genetic variants of THBS1 were significantly more frequent in patients affected by idiopathic/genetic generalized epilepsies than in non-epileptic controls. PMID: 28913875
In pulmonary hypertension TSP1-CD47 is upregulated, and contributes to pulmonary arterial vasculopathy and dysfunction. PMID: 27742621
High THBS1 expression is associated with primary effusion lymphoma. PMID: 28146424
KLK4 further liberated an N-terminal product, with purported angiogenic activity, from thrombospondin-1 (TSP1) and cleaved TSP1 in an osteoblast-derived matrix. PMID: 27378148
Data suggest pituitary cells secrete factor (TSP1) that binds to and inhibits action of BMP2 and BMP4; von Willebrand type C domain of TSP1 is likely responsible for this BMP2/4-binding activity. These studies were initially conducted using cultured cells from ovine pituitary gland and mouse cell line; interactions were confirmed using recombinant human proteins. (TSP1 = thrombospondin-1; BMP = bone morphogenetic protein) PMID: 28747434
the results obtained by combining bioinformatics and preclinical studies strongly suggest that targeting TSP-1/CD47 axis may represent a valuable therapeutic alternative for hampering melanoma spreading. PMID: 27349907
The levels of CD36, STAT3 and TGFbetaR2 mRNA were significantly higher in the TSP1-high group. PMID: 28668845
Study discovered novel and independent associations of prediabetes and related traits with MASP1, and some evidence for associations with THBS1, GPLD1 and ApoA-IV, suggesting a role for these proteins in the pathophysiology of type 2 diabetes. PMID: 27344311
TSP-1 deficiency promotes maladaptive remodelling of the ECM leading to accelerated abdominal aortic aneurysms progression. PMID: 28364044
Low THBS1 expression is associated with lung cancer. PMID: 27513329
FGF7 stimulation of cell invasion and migration was partially suppressed by the FGFR2 knockdown. In addition, FGF7/FGFR2 upregulated THBS1, and cell invasion and migration were decreased by knockdown of THBS1 PMID: 28339036
Introduces a computational model that describes the mechanistic interactions between intracellular biomolecules and cooperation between signaling pathways that together make up the complex network of TSP-1 regulation both at the transcriptional and post-transcriptional level. PMID: 28045898
Peg3 Induces Thrombospondin 1 Secretion and Inhibits Capillary Morphogenesis Independently of Beclin 1. PMID: 28174297
The simulated model of the fully calcium-loaded and calcium-depleted TSP1-Sig1 may enable the development of its interactions as a novel therapeutic target for the treatment of vascular diseases. PMID: 27485292
Regulatory elements for both IRF-1 (-1019 to -1016) and CREB (-1198 to -1195), specific to the distal THBS1 promoter, were required for leptin-induced TSP-1 transcription. PMID: 27281481
Competitive inhibition experiments with other pneumococcal hTSP-1 adhesins demonstrated that PspC and PspC-like Hic recognize similar domains, whereas PavB and Hic can bind simultaneously to hTSP-1. PMID: 28209711
Methylation of DAPK and THBS1 genes occurs in esophageal gastric-type columnar metaplasia and is associated with H. pylori cagA positive infection. PMID: 27182166
Within the limitations of this study, the results seem to sustain the involvement of Pentraxin 3 and Thrombospondin 1 in the processes of inflammation and angiogenesis in wound healing of patients with postorthodontic gingivectomy. PMID: 27403446
Circulating TSP-1 levels decrease up to 24 months prior to diagnosis of PDAC and significantly enhance the diagnostic performance of CA19-9. The influence of diabetes mellitus on biomarker behavior should be considered in future studies. PMID: 26573598
F-actin dynamics modulate the patterning of TSP1 in extracellular matrix and that TSP1 oligomer state is a key determinant of this process. PMID: 26182380
TSP-1 regulates multiple microRNAs in VSMCs adding a new layer of complexity to TSP-1 regulation of VSMC function PMID: 26728995
quercetin could increase TSP-1 expression to inhibit angiogenesis resulting in antagonizing prostate cancer PC-3 cell and xenograft tumor growth PMID: 26676551
The anti-angiogenic activity of the native TSP-1 active fragment was maintained in the new TSP-1 mimetics and the results provide a new chemical approach for the design of TSP-1 mimetics. PMID: 26464514
In the current study Factor V Leiden, prothrombin G20210A, and thrombospondin-1 polymorphisms showed no association with severity of hepatic fibrosis. PMID: 26768578
Triptolide can down regulates the expression of thrombospondin 1 and TGF-beta-1 in renal tubular cells. This plays a role in the pathogenesis of renal tubular fibrosis. PMID: 25945607
increased plasma thrombospondin-1 concentrations following ICH are independently associated with injury severity and short-term and long-term clinical outcomes PMID: 26368265
Novel role of TNFalpha in inducing inflammatory stress response in human microvascular endothelial cells through Akt- and P38 MAPK-mediated expression of TSP-1, independent of NFkappaB signaling. PMID: 25963668
Plasma thrombospondin-1 concentrations are elevated obviously and are highly associated with long-term outcome of ischemic stroke PMID: 26296896
TSP1 up regulation might be critically involved in the disease progression with rheumatoid arthritis. PMID: 25934826
The THBS1 N700S polymorphism was associated with coronary artery disease risk. PMID: 25976449
DLL4/Notch1 and BMP9 interdependent signaling induces endothelial cell quiescence via P27KIP1/thrombospondin pathway. PMID: 26471266
Authors demonstrated that TSP-1 mRNA expression level was significantly upregulated in inflamed periodontitis gingival tissues PMID: 25501558

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Subcellular Location

Secreted. Cell surface. Secreted, extracellular space, extracellular matrix. Endoplasmic reticulum. Sarcoplasmic reticulum.

Protein Families

Thrombospondin family

Database Links

HGNC: 11785

OMIM: 188060

KEGG: hsa:7057

STRING: 9606.ENSP00000260356

UniGene: Hs.164226

Code	CSB-RP073494h(N)
Abbreviation	Recombinant Human THBS1 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Thrombospondin-1 (THBS1), partial

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