Recombinant Human Thrombospondin-1(THBS1) ,partial

Code CSB-YP023487HU
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Source Yeast
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Code CSB-EP023487HU
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Source E.coli
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Code CSB-EP023487HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP023487HU
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Source Baculovirus
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Code CSB-MP023487HU
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Source Mammalian cell
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Product Details

Description
Recombinant Human Thrombospondin-1 (THBS1) is a partial length protein expressed with an N-terminal His-tagged or Tag-Free. Its purity was determined by SDS-PAGE and reached up to 85%. The activity of this protein isn’t tested. This recombinant THBS1 protein may be used to synthesize antibodies against THBS1 or on the studies of THBS1-associated signal transduction.
THBS1, a subunit of a disulfide-linked homotrimeric protein, is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to heparin, fibrinogen, fibronectin and laminin. It may play a role in dentinogenesis and/or maintenance of dentin and dental pulp. THBS1 can mediate antiangiogenic properties as a ligand for CD36. It also plays a role in ER stress response by interacting with the activating transcription factor 6 alpha (ATF6). Additionally, this protein also has been shown to play roles in platelet aggregation and tumorigenesis.
Purity >85% (SDS-PAGE)
Target Names THBS1
Uniprot No. P07996
Alternative Names THBS 1; THBS; Thbs1; Thrombospondin 1; Thrombospondin-1; TSP 1; TSP; TSP1; TSP1_HUMAN
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity).
Gene References into Functions
  1. High THBS1 expression is associated with migration, invasion, and progression of bladder cancer. PMID: 29602637
  2. intracellular dynamics of the TSP1-induced apoptosis signaling pathway PMID: 29258506
  3. TSP-1 effectively elevated P. gingivalis LPS-induced inflammation mediated by the NF-kappaB pathway and may be critical for pathology of periodontitis. PMID: 28634844
  4. These findings indicate that PRR13/THBS1 and TXN expression could be used for the prediction of resistance to treatment of epithelial ovarian cancer patients. PMID: 27779244
  5. TSP-1 appears to play an accessory role in modulating Mp activity in humans and BlaJ mice in a gender, age and muscle-dependent manner, but is unlikely a primary driver of disease progression of dysferlinopathy. PMID: 29206970
  6. TSP-4 A387P polymorphism, but not TSP-1 polymorphism, is an independent risk factor for acute myocardial infarction in Egyptians. PMID: 29336258
  7. Results show that the thrombospondin 1 (TSP1) and its receptor CD47 (CD47) axis selectively regulates NADPH oxidase 1 (Nox1) in the regulation of endothelial senescence and suggest potential targets for controlling the aging process at the molecular level. PMID: 29042481
  8. 15-LOX-1 expression in colon and prostate cancer cells leads to reduced angiogenesis. These changes could be mediated by an increase in the expression of both ICAM-1 and the anti-angiogenic protein TSP-1. PMID: 28757355
  9. miR-98 can suppress the expression of TSP1 in the peripheral B cells of patients with allergic asthma. PMID: 28760845
  10. Data indicate correlation between the levels of thrombospondin-1 and overall survival of ovarian cancer patients, suggesting thrombospondin-1 may be used as a prognostic factor in ovarian cancer patients. PMID: 28655131
  11. Studies show that thrombospondin-1 (Thbs-1), is a prolific contributor to the production and modulation of ROS in large conductance vessels and in the peripheral circulation. Recently, the presence of physiologically relevant circulating Thbs-1 levels was proven to also disrupt vasodilation to nitric oxide (NO) in coronary arterioles from aged animals, negatively impacting coronary blood flow reserve. [review] PMID: 28762749
  12. Our findings suggest that Rab37-mediated TSP1 secretion in cancer cells suppresses metastasis and angiogenesis via a cross-talk with endothelial cells and reveal a novel component of the vesicular exocytic machinery in tumor microenvironment and tumor progression PMID: 28151721
  13. TSP-1 strongly potentiated the proliferative and migratory activity of PDGF on mesenchymal stromal cells. PMID: 26992552
  14. This study demonstrated that THBS! was detected in the serums of Alzheimer disease patients. PMID: 27911324
  15. Functional studies showed THBS1 and TNC to mediate chemoresistance through the integrin beta1/mTOR pathway. PMID: 27487140
  16. These findings shed light on the mechanisms leading to beta-cell failure during metabolic stress and point to THBS1 as an interesting therapeutic target to prevent oxidative stress in type 2 diabetes. PMID: 27588705
  17. Thrombospondin-1 gene polymorphism is associated with estimated pulmonary artery pressure in patients with sickle cell anemia. PMID: 28033687
  18. High plasma levels of TSP-1 are associated with increased pulmonary arterial pressure, increased pulmonary vascular resistance and decreased survival pulmonary hypertension. PMID: 27473366
  19. Genetic variants of THBS1 were significantly more frequent in patients affected by idiopathic/genetic generalized epilepsies than in non-epileptic controls. PMID: 28913875
  20. In pulmonary hypertension TSP1-CD47 is upregulated, and contributes to pulmonary arterial vasculopathy and dysfunction. PMID: 27742621
  21. High THBS1 expression is associated with primary effusion lymphoma. PMID: 28146424
  22. KLK4 further liberated an N-terminal product, with purported angiogenic activity, from thrombospondin-1 (TSP1) and cleaved TSP1 in an osteoblast-derived matrix. PMID: 27378148
  23. Data suggest pituitary cells secrete factor (TSP1) that binds to and inhibits action of BMP2 and BMP4; von Willebrand type C domain of TSP1 is likely responsible for this BMP2/4-binding activity. These studies were initially conducted using cultured cells from ovine pituitary gland and mouse cell line; interactions were confirmed using recombinant human proteins. (TSP1 = thrombospondin-1; BMP = bone morphogenetic protein) PMID: 28747434
  24. the results obtained by combining bioinformatics and preclinical studies strongly suggest that targeting TSP-1/CD47 axis may represent a valuable therapeutic alternative for hampering melanoma spreading. PMID: 27349907
  25. The levels of CD36, STAT3 and TGFbetaR2 mRNA were significantly higher in the TSP1-high group. PMID: 28668845
  26. Study discovered novel and independent associations of prediabetes and related traits with MASP1, and some evidence for associations with THBS1, GPLD1 and ApoA-IV, suggesting a role for these proteins in the pathophysiology of type 2 diabetes. PMID: 27344311
  27. TSP-1 deficiency promotes maladaptive remodelling of the ECM leading to accelerated abdominal aortic aneurysms progression. PMID: 28364044
  28. Low THBS1 expression is associated with lung cancer. PMID: 27513329
  29. FGF7 stimulation of cell invasion and migration was partially suppressed by the FGFR2 knockdown. In addition, FGF7/FGFR2 upregulated THBS1, and cell invasion and migration were decreased by knockdown of THBS1 PMID: 28339036
  30. Introduces a computational model that describes the mechanistic interactions between intracellular biomolecules and cooperation between signaling pathways that together make up the complex network of TSP-1 regulation both at the transcriptional and post-transcriptional level. PMID: 28045898
  31. Peg3 Induces Thrombospondin 1 Secretion and Inhibits Capillary Morphogenesis Independently of Beclin 1. PMID: 28174297
  32. The simulated model of the fully calcium-loaded and calcium-depleted TSP1-Sig1 may enable the development of its interactions as a novel therapeutic target for the treatment of vascular diseases. PMID: 27485292
  33. Regulatory elements for both IRF-1 (-1019 to -1016) and CREB (-1198 to -1195), specific to the distal THBS1 promoter, were required for leptin-induced TSP-1 transcription. PMID: 27281481
  34. Competitive inhibition experiments with other pneumococcal hTSP-1 adhesins demonstrated that PspC and PspC-like Hic recognize similar domains, whereas PavB and Hic can bind simultaneously to hTSP-1. PMID: 28209711
  35. Methylation of DAPK and THBS1 genes occurs in esophageal gastric-type columnar metaplasia and is associated with H. pylori cagA positive infection. PMID: 27182166
  36. Within the limitations of this study, the results seem to sustain the involvement of Pentraxin 3 and Thrombospondin 1 in the processes of inflammation and angiogenesis in wound healing of patients with postorthodontic gingivectomy. PMID: 27403446
  37. Circulating TSP-1 levels decrease up to 24 months prior to diagnosis of PDAC and significantly enhance the diagnostic performance of CA19-9. The influence of diabetes mellitus on biomarker behavior should be considered in future studies. PMID: 26573598
  38. F-actin dynamics modulate the patterning of TSP1 in extracellular matrix and that TSP1 oligomer state is a key determinant of this process. PMID: 26182380
  39. TSP-1 regulates multiple microRNAs in VSMCs adding a new layer of complexity to TSP-1 regulation of VSMC function PMID: 26728995
  40. quercetin could increase TSP-1 expression to inhibit angiogenesis resulting in antagonizing prostate cancer PC-3 cell and xenograft tumor growth PMID: 26676551
  41. The anti-angiogenic activity of the native TSP-1 active fragment was maintained in the new TSP-1 mimetics and the results provide a new chemical approach for the design of TSP-1 mimetics. PMID: 26464514
  42. In the current study Factor V Leiden, prothrombin G20210A, and thrombospondin-1 polymorphisms showed no association with severity of hepatic fibrosis. PMID: 26768578
  43. Triptolide can down regulates the expression of thrombospondin 1 and TGF-beta-1 in renal tubular cells. This plays a role in the pathogenesis of renal tubular fibrosis. PMID: 25945607
  44. increased plasma thrombospondin-1 concentrations following ICH are independently associated with injury severity and short-term and long-term clinical outcomes PMID: 26368265
  45. Novel role of TNFalpha in inducing inflammatory stress response in human microvascular endothelial cells through Akt- and P38 MAPK-mediated expression of TSP-1, independent of NFkappaB signaling. PMID: 25963668
  46. Plasma thrombospondin-1 concentrations are elevated obviously and are highly associated with long-term outcome of ischemic stroke PMID: 26296896
  47. TSP1 up regulation might be critically involved in the disease progression with rheumatoid arthritis. PMID: 25934826
  48. The THBS1 N700S polymorphism was associated with coronary artery disease risk. PMID: 25976449
  49. DLL4/Notch1 and BMP9 interdependent signaling induces endothelial cell quiescence via P27KIP1/thrombospondin pathway. PMID: 26471266
  50. Authors demonstrated that TSP-1 mRNA expression level was significantly upregulated in inflamed periodontitis gingival tissues PMID: 25501558

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Subcellular Location Endoplasmic reticulum, Sarcoplasmic reticulum
Protein Families Thrombospondin family
Database Links

HGNC: 11785

OMIM: 188060

KEGG: hsa:7057

STRING: 9606.ENSP00000260356

UniGene: Hs.164226

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