Code | CSB-E04724h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Have Questions? | Leave a Message or Start an on-line Chat |
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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1:1 |
Average % |
96 |
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Range % |
85-99 |
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1:2 |
Average % |
92 |
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Range % |
87-105 |
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1:4 |
Average % |
95 |
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Range % |
92-99 |
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1:8 |
Average % |
89 |
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Range % |
86-98 |
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The recovery of human TGF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type |
Average % Recovery |
Range |
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Serum (n=5) |
95 |
89-98 |
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EDTA plasma (n=4) |
93 |
86-99 |
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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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4000 |
1.875 |
1.807 |
1.841 |
1.711 |
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2000 |
1.523 |
1.495 |
1.509 |
1.379 |
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1000 |
1.170 |
1.126 |
1.148 |
1.018 |
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500 |
0.854 |
0.840 |
0.847 |
0.717 |
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250 |
0.626 |
0.614 |
0.620 |
0.490 |
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125 |
0.413 |
0.422 |
0.418 |
0.288 |
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62.5 |
0.289 |
0.296 |
0.293 |
0.163 |
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0 |
0.131 |
0.128 |
0.130 |
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This Human TGFA ELISA Kit was designed for the quantitative measurement of Human TGFA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 5.48 pg/ml-4000 pg/ml and the sensitivity is 5.48 pg/ml.
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Regarding your ELISA kit CSB-E04724h, could you please advise whether or not this kit is suitable for use with cell culture supernatant samples?