Transforming Growth Factor Alpha (TGFA), also known as TGF-α, is a mitogenic polypeptide that functions as a ligand for the epidermal growth factor receptor (EGFR). It activates downstream pathways including MAPK/ERK and PI3K/AKT through receptor dimerization and tyrosine kinase phosphorylation, promoting epithelial cell proliferation, differentiation, and survival. TGFA plays critical roles in embryonic development, wound healing, and tissue regeneration. Its expression is tightly regulated under physiological conditions but becomes dysregulated in multiple pathological contexts, particularly in cancers characterized by EGFR hyperactivation.
Overexpression of TGFA correlates with tumorigenesis in colorectal, lung, and hepatocellular carcinomas through sustained EGFR signaling. It also contributes to inflammatory skin disorders such as psoriasis via aberrant keratinocyte activation. Therapeutic strategies targeting TGFA-associated pathways include EGFR monoclonal antibodies (e.g., cetuximab) and tyrosine kinase inhibitors (erlotinib, gefitinib), though resistance mechanisms remain challenging. Recent trials explore combination therapies with MEK or STAT3 inhibitors to overcome resistance. Diagnostic applications leverage TGFA as a biomarker for EGFR-driven malignancies and therapeutic response monitoring.
TGFA Antibody (CSB-PA07294A0Rb)
Validated Data
Western blot
All lanes: TGFA antibody at 2µg/ml
Lane 1: EC109 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 18, 17 kDa
Observed band size: 30, 35 kDa
Immunofluorescent analysis of Hela cells using CSB-PA07294A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA07294A0Rb at dilution of 1:100
Human transforming growth factor α,TGF-α ELISA Kit (CSB-E04724h)
Validated Data
Code: CSB-E04724h
Size: 48T/ 96T
Sensitivity:
Detection Range:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The following TGFA reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
TGFA Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA07294A0Rb | TGFA Antibody | Human | ELISA, WB, IHC, IF |
CSB-PA799735 | TGFA Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA004275 | TGFA Antibody | Human,Mouse,Rat,Monkey | WB, IHC, ELISA |
CSB-PA582653 | TGFA Antibody | Human,Mouse,Rat | ELISA,IHC |
TGFA Antibodies for Ovis aries (Sheep)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA023445ZA01SH | TGFA Antibody | Ovis aries | ELISA, WB (ensure identification of antigen) |
TGFA Proteins for Ovis aries (Sheep)
Code | Product Name | Source |
---|---|---|
CSB-EP023445SH | Recombinant Sheep Protransforming growth factor alpha (TGFA), partial | E.coli |
CSB-YP023445SH | Recombinant Sheep Protransforming growth factor alpha (TGFA), partial | Yeast |
CSB-BP023445SH CSB-MP023445SH CSB-EP023445SH-B |
Recombinant Sheep Protransforming growth factor alpha (TGFA), partial | Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TGFA Proteins for Oryctolagus cuniculus (Rabbit)
Code | Product Name | Source |
---|---|---|
CSB-YP023445RB CSB-EP023445RB CSB-BP023445RB CSB-MP023445RB CSB-EP023445RB-B |
Recombinant Rabbit Transforming growth factor alpha (TGFA) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TGFA Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP023445MO CSB-EP023445MO CSB-BP023445MO CSB-MP023445MO CSB-EP023445MO-B |
Recombinant Mouse Protransforming growth factor alpha (Tgfa), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TGFA Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP360628RA CSB-BP360628RA CSB-MP360628RA CSB-EP360628RA-B |
Recombinant Rat Protransforming growth factor alpha (Tgfa), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TGFA Proteins for Macaca mulatta (Rhesus macaque)
Code | Product Name | Source |
---|---|---|
CSB-CF023445MOW | Recombinant Macaca mulatta (Rhesus macaque) Protransforming growth factor alpha (TGFA) | in vitro E.coli expression system |
CSB-YP023445MOW1 CSB-EP023445MOW1 CSB-BP023445MOW1 CSB-MP023445MOW1 CSB-EP023445MOW1-B |
Recombinant Macaca mulatta (Rhesus macaque) Protransforming growth factor alpha (TGFA), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TGFA Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-CF023445HU | Recombinant Human Protransforming growth factor alpha (TGFA) | in vitro E.coli expression system |
CSB-EP023445HU1 | Recombinant Human Protransforming growth factor alpha (TGFA), partial,Escherichia Coli | E.coli |
CSB-BP023445HU1 | Recombinant Human Protransforming growth factor alpha (TGFA), partial,Baculovirus | Baculovirus |
CSB-MP023445HU1 | Recombinant Human Protransforming growth factor alpha (TGFA), partial,Mammalian cell | Mammalian cell |
CSB-YP023445HU1 | Recombinant Human Protransforming growth factor alpha (TGFA), partial,Yeast | Yeast |
CSB-EP072944h CSB-EP072944h-B |
Recombinant Human Protransforming growth factor alpha (TGFA) | E.coli In Vivo Biotinylation in E.coli |
CSB-EP023445HU1-B |
Recombinant Human Protransforming growth factor alpha (TGFA) | In Vivo Biotinylation in E.coli |
TGFA ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E04724h | Human transforming growth factor α,TGF-α ELISA Kit | serum, plasma, tissue homogenates |
TGFA ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E07290m | Mouse transforming growth factor α,TGF-α ELISA Kit | serum, plasma, cell culture supernates, tissue homogenates | 3.12 pg/mL |
TGFA ELISA Kit for Rattus norvegicus (Rat)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E07289r | Rat transforming growth factor α,TGF-α ELISA Kit | serum, plasma, tissue homogenates | 1.95 pg/ml |