Human tryptophanyl-tRNA synthetase,WARS ELISA Kit

Code CSB-E11789h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The product CSB-E11789h is a sandwich ELISA kit developed to measure levels of human tryptophanyl-tRNA synthetase (WARS) in serum, plasma, cell lysates, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of WARS bound in the initial step.

WARS is an essential enzyme because it catalyzes the ligation of tryptophan (Trp) to its cognate tRNAtrp during translation via aminoacylation. It is localized not only to the nucleus but also to the extracellular space, playing an important role in innate immunity, angiogenesis, and IFN-γ signaling. WARS expression varies significantly in different tissues and pathological states, implying that it plays unique roles in physiological homeostasis and immune defense. It is also involved in the pathogenesis of multiple diseases, including sepsis, cancer, and autoimmune and brain diseases.

Target Name tryptophanyl-tRNA synthetase
Alternative Names
Gamma 2 ELISA Kit; GAMMA-2 ELISA Kit; Gamma2 ELISA Kit; hWRS ELISA Kit; IFI 53 ELISA Kit; IFI53 ELISA Kit; IFP 53 ELISA Kit; IFP53 ELISA Kit; Interferon induced protein 53 ELISA Kit; Interferon-induced protein 53 ELISA Kit; SYWC_HUMAN ELISA Kit; T2-TrpRS ELISA Kit; TrpRS ELISA Kit; Tryptophan tRNA ligase; cytoplasmic ELISA Kit; Tryptophan tRNA ligase 1 cytoplasmic ELISA Kit; Tryptophan tRNA ligase ELISA Kit; Tryptophan--tRNA ligase ELISA Kit; Tryptophanyl tRNA synthetase ELISA Kit; Tryptophanyl tRNA synthetase cytoplasmic ELISA Kit; WARS ELISA Kit; WARS protein ELISA Kit; WRS ELISA Kit
Abbreviation WARS
Uniprot No. P23381
Species Homo sapiens (Human)
Sample Types serum, plasma, cell lysates, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human WARS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 88-95  
1:2 Average % 87  
Range % 81-93  
1:4 Average % 97  
Range % 93-101  
1:8 Average % 100  
Range % 94-106  
Recovery
The recovery of human WARS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 84-95  
EDTA plasma (n=4) 93 88-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 1.972 2.050 2.011 1.920  
10 1.446 1.556 1.501 1.410  
5 1.027 1.101 1.064 0.973  
2.5 0.638 0.606 0.622 0.531  
1.25 0.381 0.387 0.384 0.293  
0.625 0.293 0.275 0.284 0.193  
0.312 0.195 0.201 0.198 0.107  
0 0.093 0.089 0.091    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
Gene References into Functions
  1. Based on these results, secretion of full-length tryptophanyl-tRNA synthetase appears to work as a primary defence system against infection, acting before full activation of innate immunity. PMID: 27748732
  2. findings establish WARS as a gene whose mutations may cause distal hereditary motor neuropathy and alter canonical and non-canonical functions of tryptophanyl-tRNA synthetase. PMID: 28369220
  3. Overexpression of WARS predicts no recurrence and good survival for triple-negative breast cancer patients. PMID: 26209610
  4. Tryptophanyl-tRNA synthetase expression is up-regulated in patients with rheumatoid arthritis. PMID: 24515434
  5. Genes within recently identified loci associated with waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression, which correlates with obesity-related traits. Adipose tissue (AT) mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3, GRB14) PMID: 23670221
  6. Indoleamine2,3-dioxygenase and tryptophanyl-tRNA synthetase may play critical roles in the immune pathogenesis of chronic kidney disease. PMID: 23651343
  7. Naturally occurring fragments of the two proteins involved in translation, TyrRS and TrpRS, have opposing activities on angiogenesis. PMID: 21442253
  8. Tryptophanyl-tRNA synthetase down-regulation by hypoxia may be a factor responsible for low TrpRS in pancreatic tumors with high metastatic ability. PMID: 21926542
  9. Mini-tryptophanyl-tRNA synthetase inhibited ischemic angiogenesis in rats. PMID: 20963594
  10. Tryptophanyl-tRNA synthetase is a multidomain protein exhibiting excellent allosteric communication, and this research has provided valuable structural as well as functional insights into the protein. PMID: 19768679
  11. Low tryptophanyl-tRNA synthetase is associated with recurrence in colorectal cancer. PMID: 19900940
  12. In this study, we show that a recombinant form of a COOH-terminal fragment of TrpRS is a potent antagonist of vascular endothelial growth factor-induced angiogenesis in a mouse model and of naturally occurring retinal angiogenesis in the neonatal mouse PMID: 11773625
  13. Thus, protein synthesis may be linked to the regulation of angiogenesis by a natural fragment of TrpRS PMID: 11773626
  14. Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains PMID: 11834741
  15. The recently discovered antiangiogenic and cell-signaling activities of tryptophanyl-tRNA synthetase bioactive fragments are discussed in this review. PMID: 12416978
  16. TrpRS may have a role in the maintenance of vascular homeostasis PMID: 14630953
  17. results suggest that mammalian and bacterial tryptophanyl-tRNA synthetase might use different mechanisms to recognize the substrate and modeling studies indicate that transfer RNA binds with the dimeric enzyme PMID: 14660560
  18. A crystal structure of human tryptophanyl-tRNA synthetase was solved at 2.1 A with a tryptophanyl-adenylate bound at the active site PMID: 14671330
  19. May play an important role in the intracellular regulation of protein synthesis under conditions of oxidative stress. PMID: 15628863
  20. These crystals captured two conformations of the human tryptophanyl-tRNA synthetase and tRNATrp complex, which are nearly identical with respect to the protein and a bound tryptophan. PMID: 16724112
  21. The first crystal structure of human tryptophanyl-tRNA synthetase (hTrpRS) in complex with tRNA(Trp) and Trp which, together with biochemical data, reveals the molecular basis of a novel tRNA binding and recognition mechanism. PMID: 16798914
  22. Results provide the first evidence of the involvement of heme in regulation of TrpRS aminoacylation activity. PMID: 17877375
  23. the annexin II-S100A10 complex, which regulates exocytosis, forms a ternary complex with TrpRS. PMID: 17999956
  24. Analysis of the molecular basis of the mechanisms of the substrate recognition and the activation reaction by tryptophanyl-tRNA synthetase. PMID: 18180246
  25. Indoleamine 2,3-dioxygenase (IDO)-expression in antigen-presenting cells (APCs) may control autoimmune responses by depleting the available tryptophan, whereas tryptophanyl-tRNA synthetase (TTS) may counteract this effect PMID: 19363598

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Subcellular Location Cytoplasm.
Protein Families Class-I aminoacyl-tRNA synthetase family
Database Links

HGNC: 12729

OMIM: 191050

KEGG: hsa:7453

STRING: 9606.ENSP00000347495

UniGene: Hs.497599

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