Recombinant Human Tryptophan--tRNA ligase, cytoplasmic(WARS)

Code CSB-YP025965HU
Size US$1916
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names WARS
Uniprot No. P23381
Research Area Epigenetics and Nuclear Signaling
Alternative Names Gamma 2; GAMMA-2; Gamma2; hWRS; IFI 53; IFI53; IFP 53; IFP53; Interferon induced protein 53; Interferon-induced protein 53; SYWC_HUMAN; T2-TrpRS; TrpRS; Tryptophan tRNA ligase; cytoplasmic; Tryptophan tRNA ligase 1 cytoplasmic; Tryptophan tRNA ligase; Tryptophan--tRNA ligase; Tryptophanyl tRNA synthetase ; Tryptophanyl tRNA synthetase cytoplasmic; WARS; WARS protein; WRS
Species Homo sapiens (Human)
Source Yeast
Expression Region 2-471aa
Target Protein Sequence PNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGGNCDVDVSFMYLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRKEVTDEIVKEFMTPRKLSFDFQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 56.5 kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
Gene References into Functions
  1. Based on these results, secretion of full-length tryptophanyl-tRNA synthetase appears to work as a primary defence system against infection, acting before full activation of innate immunity. PMID: 27748732
  2. findings establish WARS as a gene whose mutations may cause distal hereditary motor neuropathy and alter canonical and non-canonical functions of tryptophanyl-tRNA synthetase. PMID: 28369220
  3. Overexpression of WARS predicts no recurrence and good survival for triple-negative breast cancer patients. PMID: 26209610
  4. Tryptophanyl-tRNA synthetase expression is up-regulated in patients with rheumatoid arthritis. PMID: 24515434
  5. Genes within recently identified loci associated with waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression, which correlates with obesity-related traits. Adipose tissue (AT) mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3, GRB14) PMID: 23670221
  6. Indoleamine2,3-dioxygenase and tryptophanyl-tRNA synthetase may play critical roles in the immune pathogenesis of chronic kidney disease. PMID: 23651343
  7. Naturally occurring fragments of the two proteins involved in translation, TyrRS and TrpRS, have opposing activities on angiogenesis. PMID: 21442253
  8. Tryptophanyl-tRNA synthetase down-regulation by hypoxia may be a factor responsible for low TrpRS in pancreatic tumors with high metastatic ability. PMID: 21926542
  9. Mini-tryptophanyl-tRNA synthetase inhibited ischemic angiogenesis in rats. PMID: 20963594
  10. Tryptophanyl-tRNA synthetase is a multidomain protein exhibiting excellent allosteric communication, and this research has provided valuable structural as well as functional insights into the protein. PMID: 19768679
  11. Low tryptophanyl-tRNA synthetase is associated with recurrence in colorectal cancer. PMID: 19900940
  12. In this study, we show that a recombinant form of a COOH-terminal fragment of TrpRS is a potent antagonist of vascular endothelial growth factor-induced angiogenesis in a mouse model and of naturally occurring retinal angiogenesis in the neonatal mouse PMID: 11773625
  13. Thus, protein synthesis may be linked to the regulation of angiogenesis by a natural fragment of TrpRS PMID: 11773626
  14. Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains PMID: 11834741
  15. The recently discovered antiangiogenic and cell-signaling activities of tryptophanyl-tRNA synthetase bioactive fragments are discussed in this review. PMID: 12416978
  16. TrpRS may have a role in the maintenance of vascular homeostasis PMID: 14630953
  17. results suggest that mammalian and bacterial tryptophanyl-tRNA synthetase might use different mechanisms to recognize the substrate and modeling studies indicate that transfer RNA binds with the dimeric enzyme PMID: 14660560
  18. A crystal structure of human tryptophanyl-tRNA synthetase was solved at 2.1 A with a tryptophanyl-adenylate bound at the active site PMID: 14671330
  19. May play an important role in the intracellular regulation of protein synthesis under conditions of oxidative stress. PMID: 15628863
  20. These crystals captured two conformations of the human tryptophanyl-tRNA synthetase and tRNATrp complex, which are nearly identical with respect to the protein and a bound tryptophan. PMID: 16724112
  21. The first crystal structure of human tryptophanyl-tRNA synthetase (hTrpRS) in complex with tRNA(Trp) and Trp which, together with biochemical data, reveals the molecular basis of a novel tRNA binding and recognition mechanism. PMID: 16798914
  22. Results provide the first evidence of the involvement of heme in regulation of TrpRS aminoacylation activity. PMID: 17877375
  23. the annexin II-S100A10 complex, which regulates exocytosis, forms a ternary complex with TrpRS. PMID: 17999956
  24. Analysis of the molecular basis of the mechanisms of the substrate recognition and the activation reaction by tryptophanyl-tRNA synthetase. PMID: 18180246
  25. Indoleamine 2,3-dioxygenase (IDO)-expression in antigen-presenting cells (APCs) may control autoimmune responses by depleting the available tryptophan, whereas tryptophanyl-tRNA synthetase (TTS) may counteract this effect PMID: 19363598

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Subcellular Location Cytoplasm
Protein Families Class-I aminoacyl-tRNA synthetase family
Database Links

HGNC: 12729

OMIM: 191050

KEGG: hsa:7453

STRING: 9606.ENSP00000347495

UniGene: Hs.497599

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