Human tumor necrosis factor-related apoptosis-inducing ligand receptor 4(TRAIL-R4) ELISA Kit

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Target Data

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Name: Human tumor necrosis factor-related apoptosis-inducing ligand receptor 4(TRAIL-R4) ELISA Kit
Brand: CUSABIO
SKU: CSB-E04749h

Product Details

Target Name

tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain

Alternative Names

CD 264 ELISA Kit; CD264 ELISA Kit; DcR 2 ELISA Kit; DcR2 ELISA Kit; Decoy receptor 2 ELISA Kit; Decoy with truncated death domain ELISA Kit; OTTHUMP00000123528 ELISA Kit; TNF receptor related receptor for TRAIL ELISA Kit; TNF related apoptosis inducing ligand receptor 4 ELISA Kit; TNF-related apoptosis-inducing ligand receptor 4 ELISA Kit; TNFRSF 10D ELISA Kit; TNFRSF10D ELISA Kit; TR10D_HUMAN ELISA Kit; TRAIL R4 ELISA Kit; TRAIL receptor 4 ELISA Kit; TRAIL receptor with a truncated death domain ELISA Kit; TRAIL-R4 ELISA Kit; TRAILR4 ELISA Kit; TRUNDD ELISA Kit; Tumor necrosis factor receptor superfamily member 10D ELISA Kit; Tumor necrosis factor receptor superfamily; member 10d; decoy with truncated death domain ELISA Kit

Abbreviation

TNFRSF10D

Uniprot No.

Q9UBN6

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TRAIL-R4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-92
1:2	Average %	95
	Range %	91-99
1:4	Average %	98
	Range %	92-104
1:8	Average %	92
	Range %	82-95

Recovery

The recovery of human TRAIL-R4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-101
EDTA plasma (n=4)	107	102-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.214	2.323	2.269	2.112
10	1.597	1.486	1.542	1.385
5	0.957	0.886	0.922	0.765
2.5	0.547	0.568	0.558	0.401
1.25	0.355	0.366	0.361	0.204
0.625	0.281	0.297	0.289	0.132
0.312	0.208	0.214	0.211	0.054
0	0.156	0.157	0.157

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Target Data

Function	Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed
Gene References into Functions	The high-dose hook effect was apparent during ELISA testing of uDcR2 in chronic kidney disease (CKD) patients, yet dilution of the urine samples neutralized this effect. However, the use of a four-fold dilution of urine for uDcR2/cre testing may eliminate the high-dose hook effect and make it possible to effectively monitor the severity of TII in CKD patients. PMID: 29879421 results suggest that CD264 is a surface marker of cellular age for bone marrow-derived mesenchymal stem cells. PMID: 28962588 Urinary DcR2 could potentially serve as a novel biomarker for tubulointerstitial injury and may reflect senescence of renal proximal tubular cells in diabetic nephropathy. PMID: 28356293 Results identified epigenetic inactivation of TNFRSF10C and TNFRSF10D in majority of cervical cancer cases. PMID: 26542757 membrane expression more common in endometrioid endometrial cancer than in normal endometrium PMID: 24649804 This study identified TNFRSF10D DNA methylation status as an independent prognostic biomarker for relapse-free survival and overall mortality in non-metastatic melanoma patients. PMID: 25003639 The membrane expression of the TRAIL receptors DR4, DR5, DcR1 and DcR2 is greater in normal endometrium than endometrioid adenocarcinoma (EAC). The level of the receptors in EAC is not dependent on grading and staging and does not predict survival. PMID: 23584885 the results presented here claim for a relevant impact of aberrant methylation of decoy receptors in melanoma and allow to understand how the silencing of DcR1 and DcR2 is related to melanomagenesis. PMID: 24211571 TRAIL receptor-4 expression profiles on T cells might be important in revelation of rheumatoid arthritis pathogenesis. PMID: 20799941 ANT2 shRNA treatment sensitized MCF7, T47 D, and BT474 cells to TRAIL-induced apoptosis by up-regulating the expression of TRAIL death receptors 4 and 5 (DR4 and DR5) and down-regulating the TRAIL decoy receptor 2 (DcR2). PMID: 20875141 these results demonstrated that hypoxia-inducible factor 1alpha played a crucial role in regulating the transcription of DcR2. PMID: 20018172 Enhanced expression of DcR2 promotes peripheral blood eosinophil survival in the airways of allergic asthmatics following segmental antigen challenge. PMID: 12421985 Cytotoxicity and apoptosis induced by TRAIL to beta-cell lines CM were inhibited competitively by soluble TRAIL receptors, R1, R2, R3 or R4. PMID: 12488957 Respiratory syncytial virus infection strongly up-regulated the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its functional receptors death receptor 4 (DR4) and DR5. PMID: 12915532 Our results demonstrate that DcR1 and DcR2 genes are frequently methylated in various tumor types and aberrant methylation was the cause for silencing of DcR1 and DcR2 expression. PMID: 14999791 The DcR2 was found to have a truncated and non-functional death domain. PMID: 15538968 CD8+ lymphocytes and NKT lymphocytes, but not CD4+ lymphocytes, express TRAIL-R4 PMID: 15919363 Resistance to TRAIL-induced apoptosis in acute myeloid leukemia cells is associated with expression of TRAIL-R4. PMID: 15921376 Preligand assembly domain-mediated ligand-independent association between TRAIL receptor 4 (TR4) and TR2 regulates TRAIL-induced apoptosis. PMID: 16319225 DCR2 was found positive in 81 and 33% normal, 46 and 10% nodular hyperplasia, 74 and 36% PIN tissues, 87 and 89% low-grade carcinomas, and 100 and 93% high-grade carcinomas PMID: 16799475 TRAIL-R4-beta is a new splice variant of TRAIL-receptor 4 lacking the cysteine rich domain 1 PMID: 16934748 The specificity of DcR1- and DcR2-mediated TRAIL inhibition reveals an additional level of complexity for the regulation of TRAIL signaling. PMID: 16980609 CASP8, DCR2, and HIN-1 methylation leads to progression of neuroblastoma PMID: 17545522 These data strongly support a recent proposal that a segment at 8p21.3 contains crucial prostate cancer tumor suppressors. PMID: 18460741 DCR2-methylated patients showed significantly poorer 5-year event-free survival in the whole neuroblastoma group (43% PMID: 18980997 High TRAIL death receptor 4 and decoy receptor 2 expression correlates with significant cell death in pancreatic ductal adenocarcinoma patients. PMID: 18981952 Results indicate that disc cells, after herniation, undergo apoptotic cell death via the DR5/TRAIL pathway. PMID: 19730199 Show More Hide All
Subcellular Location	Membrane, Single-pass type I membrane protein
Tissue Specificity	Widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. Also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart.
Database Links	HGNC: 11907 OMIM: 603614 KEGG: hsa:8793 STRING: 9606.ENSP00000310263 UniGene: Hs.213467

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Check Code：

Code	CSB-E04749h
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.