Product Details

Uniprot No.

Target Names

Alternative Names

CD 264 antibody; CD264 antibody; DcR 2 antibody; DcR2 antibody; Decoy receptor 2 antibody; Decoy with truncated death domain antibody; OTTHUMP00000123528 antibody; TNF receptor related receptor for TRAIL antibody; TNF related apoptosis inducing ligand receptor 4 antibody; TNF-related apoptosis-inducing ligand receptor 4 antibody; TNFRSF 10D antibody; TNFRSF10D antibody; TR10D_HUMAN antibody; TRAIL R4 antibody; TRAIL receptor 4 antibody; TRAIL receptor with a truncated death domain antibody; TRAIL-R4 antibody; TRAILR4 antibody; TRUNDD antibody; Tumor necrosis factor receptor superfamily member 10D antibody; Tumor necrosis factor receptor superfamily; member 10d; decoy with truncated death domain antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human TNFRSF10D protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

21F5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

TNFRSF10D, also known as TRAIL-R4 or DcR2, functions as a decoy receptor within the TRAIL signaling pathway, playing a critical regulatory role in apoptosis. Unlike death-inducing TRAIL receptors, TNFRSF10D contains a truncated death domain that prevents it from transmitting apoptotic signals, effectively competing for TRAIL binding and protecting cells from programmed cell death. This protective mechanism has significant implications for cancer research, where tumor cells may exploit decoy receptor expression to evade TRAIL-mediated immune surveillance.

This recombinant monoclonal antibody, generated from clone 21F5 in rabbit host, offers the consistency and reliability that demanding research applications require. Because the antibody sequence is defined and produced through recombinant technology, you can expect reproducible performance across experiments and between lots, eliminating the variability that can complicate long-term studies or multi-site collaborations. The antibody was raised against a synthesized peptide derived from human TNFRSF10D protein, ensuring specificity for your target of interest.

Validation in immunohistochemistry demonstrates effective performance in paraffin-embedded human tissue sections. Testing in human liver cancer tissue using a Leica Bond system with citrate buffer antigen retrieval and HRP-polymer detection confirmed clear staining at 1:100 dilution, with recommended working dilutions ranging from 1:50 to 1:200 depending on your specific tissue and detection system. The antibody is also validated for ELISA applications, providing flexibility across your experimental workflow.

Supplied in a stabilized liquid format with glycerol for convenient storage at -20°C or -80°C, this antibody is well-suited for researchers investigating TRAIL pathway regulation, apoptosis resistance mechanisms, and tumor biology.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway.

Gene References into Functions

The high-dose hook effect was apparent during ELISA testing of uDcR2 in chronic kidney disease (CKD) patients, yet dilution of the urine samples neutralized this effect. However, the use of a four-fold dilution of urine for uDcR2/cre testing may eliminate the high-dose hook effect and make it possible to effectively monitor the severity of TII in CKD patients. PMID: 29879421
results suggest that CD264 is a surface marker of cellular age for bone marrow-derived mesenchymal stem cells. PMID: 28962588
Urinary DcR2 could potentially serve as a novel biomarker for tubulointerstitial injury and may reflect senescence of renal proximal tubular cells in diabetic nephropathy. PMID: 28356293
Results identified epigenetic inactivation of TNFRSF10C and TNFRSF10D in majority of cervical cancer cases. PMID: 26542757
membrane expression more common in endometrioid endometrial cancer than in normal endometrium PMID: 24649804
This study identified TNFRSF10D DNA methylation status as an independent prognostic biomarker for relapse-free survival and overall mortality in non-metastatic melanoma patients. PMID: 25003639
The membrane expression of the TRAIL receptors DR4, DR5, DcR1 and DcR2 is greater in normal endometrium than endometrioid adenocarcinoma (EAC). The level of the receptors in EAC is not dependent on grading and staging and does not predict survival. PMID: 23584885
the results presented here claim for a relevant impact of aberrant methylation of decoy receptors in melanoma and allow to understand how the silencing of DcR1 and DcR2 is related to melanomagenesis. PMID: 24211571
TRAIL receptor-4 expression profiles on T cells might be important in revelation of rheumatoid arthritis pathogenesis. PMID: 20799941
ANT2 shRNA treatment sensitized MCF7, T47 D, and BT474 cells to TRAIL-induced apoptosis by up-regulating the expression of TRAIL death receptors 4 and 5 (DR4 and DR5) and down-regulating the TRAIL decoy receptor 2 (DcR2). PMID: 20875141
these results demonstrated that hypoxia-inducible factor 1alpha played a crucial role in regulating the transcription of DcR2. PMID: 20018172
Enhanced expression of DcR2 promotes peripheral blood eosinophil survival in the airways of allergic asthmatics following segmental antigen challenge. PMID: 12421985
Cytotoxicity and apoptosis induced by TRAIL to beta-cell lines CM were inhibited competitively by soluble TRAIL receptors, R1, R2, R3 or R4. PMID: 12488957
Respiratory syncytial virus infection strongly up-regulated the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its functional receptors death receptor 4 (DR4) and DR5. PMID: 12915532
Our results demonstrate that DcR1 and DcR2 genes are frequently methylated in various tumor types and aberrant methylation was the cause for silencing of DcR1 and DcR2 expression. PMID: 14999791
The DcR2 was found to have a truncated and non-functional death domain. PMID: 15538968
CD8+ lymphocytes and NKT lymphocytes, but not CD4+ lymphocytes, express TRAIL-R4 PMID: 15919363
Resistance to TRAIL-induced apoptosis in acute myeloid leukemia cells is associated with expression of TRAIL-R4. PMID: 15921376
Preligand assembly domain-mediated ligand-independent association between TRAIL receptor 4 (TR4) and TR2 regulates TRAIL-induced apoptosis. PMID: 16319225
DCR2 was found positive in 81 and 33% normal, 46 and 10% nodular hyperplasia, 74 and 36% PIN tissues, 87 and 89% low-grade carcinomas, and 100 and 93% high-grade carcinomas PMID: 16799475
TRAIL-R4-beta is a new splice variant of TRAIL-receptor 4 lacking the cysteine rich domain 1 PMID: 16934748
The specificity of DcR1- and DcR2-mediated TRAIL inhibition reveals an additional level of complexity for the regulation of TRAIL signaling. PMID: 16980609
CASP8, DCR2, and HIN-1 methylation leads to progression of neuroblastoma PMID: 17545522
These data strongly support a recent proposal that a segment at 8p21.3 contains crucial prostate cancer tumor suppressors. PMID: 18460741
DCR2-methylated patients showed significantly poorer 5-year event-free survival in the whole neuroblastoma group (43% PMID: 18980997
High TRAIL death receptor 4 and decoy receptor 2 expression correlates with significant cell death in pancreatic ductal adenocarcinoma patients. PMID: 18981952
Results indicate that disc cells, after herniation, undergo apoptotic cell death via the DR5/TRAIL pathway. PMID: 19730199

Hide All

Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. Also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart.

Database Links

HGNC: 11907

OMIM: 603614

KEGG: hsa:8793

STRING: 9606.ENSP00000310263

UniGene: Hs.213467

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Code	CSB-RA160607A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA160607A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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TNFRSF10D Recombinant Monoclonal Antibody

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Target Background