Human very low density lipoprotein(VLDL)ELISA Kit

Human very low density lipoprotein(VLDL)ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Human very low density lipoprotein(VLDL)ELISA Kit

Citations (4) Protocol

Code	CSB-E17087h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
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Product Details
Citations (4)
Related Products
Customer Reviews and Q&A

Target Name

very low density lipoprotein(VLDL)

Alternative Names

N/A

Abbreviation

VLDL

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.78 μg/mL-50 μg/mL

Sensitivity

0.195 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	97
	Range %	91-103
1:400	Average %	95
	Range %	91-99
1:800	Average %	94
	Range %	88-98
1:1600	Average %	105
	Range %	101-109

Recovery

The recovery of human VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	103	98-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
50	0.086	0.088	0.087
25	0.140	0.146	0.143
12.5	0.229	0.234	0.232
6.25	0.338	0.348	0.343
3.12	0.576	0.554	0.565
1.56	0.991	0.927	0.959
0.78	1.392	1.354	1.373
0	2.568	2.684	2.626

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

The human VLDL ELISA Kit is used to quantitatively measure human VLDL concentrations in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the competitive ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops negatively to the amount of VLDL in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

VLDL is made by the liver and released into the bloodstream. It is a lipoprotein rich in triacylglycerols (TAGs). VLDL is commonly regarded as bad cholesterol. The body needs some VLDL to run properly. VLDL carries different types of fats, primarily circulatory triglycerides, to cells and tissues. However, excess VLDL can increase the risk of developing health conditions, including heart failure. Overproduction or delayed clearance of VLDL is considered as causing hypertriglyceridemia. VLDL is involved in cardiac remodeling but also causes ventricular function impairment. VLDL engagement with its receptor VLDLR can induce the development of atherosclerosis.

Angiopoietin-like protein 4 promotes very-low-density lipoprotein assembly and secretion in bovine hepatocytes in vitro. Y Kong,IUBMB Life,2020
The adverse effects of long-term l-carnitine supplementation on liver and kidney function in rats Liu L et al,Hum Exp Toxicol,2015
In vitro effects of active components of Polygonum Multiflorum Radix on enzymes involved in the lipid metabolism Wang W et al,J Ethnopharmacol,2014
A Preliminary Comparative Assessment of Apelin Serum Levels in Persistent Atrial Fibrillation and Coronary Artery Disease Elnaz Khorrami.et al,/,2013

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.