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| Code | CSB-E17087h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:1600 | Average % | 105 | ||||
| Range % | 101-109 | |||||
| The recovery of human VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-95 | ||||
| EDTA plasma (n=4) | 103 | 98-107 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human VLDL ELISA Kit is used to quantitatively measure human VLDL concentrations in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the competitive ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops negatively to the amount of VLDL in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
VLDL is made by the liver and released into the bloodstream. It is a lipoprotein rich in triacylglycerols (TAGs). VLDL is commonly regarded as bad cholesterol. The body needs some VLDL to run properly. VLDL carries different types of fats, primarily circulatory triglycerides, to cells and tissues. However, excess VLDL can increase the risk of developing health conditions, including heart failure. Overproduction or delayed clearance of VLDL is considered as causing hypertriglyceridemia. VLDL is involved in cardiac remodeling but also causes ventricular function impairment. VLDL engagement with its receptor VLDLR can induce the development of atherosclerosis.
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