Rat very low density lipoprotein(VLDL)ELISA Kit

Code CSB-E17088r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
very low density lipoprotein(VLDL)
Alternative Names
N/A
Abbreviation
VLDL
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 ng/mL-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %99
Range %91-103
1:200Average %87
Range %81-92
1:400Average %104
Range %98-107
1:800Average %101
Range %92-105
Recovery
The recovery of rat VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9486-99
EDTA plasma (n=4)9891-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
2000.084 0.088 0.086
1000.152 0.159 0.156
500.226 0.234 0.230
250.389 0.378 0.384
12.50.613 0.635 0.624
6.250.830 0.843 0.837
3.121.138 1.117 1.128
01.970 1.869 1.920
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat VLDL ELISA Kit was designed for the quantitative measurement of Rat VLDL protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

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