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Monkey Interferon γ,IFN-γ ELISA Kit

Protocol MSDS
Code CSB-E10049Mo
Size 96T,5×96T,10×96T
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Product Details

Target Name
interferon, gamma
Alternative Names
IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
Abbreviation
IFNG
Uniprot No.
P63310
Species
Monkey
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20001.830 1.990 1.910 1.805
10001.496 1.398 1.447 1.342
5001.133 1.138 1.136 1.031
2500.932 0.902 0.917 0.812
1250.544 0.535 0.540 0.435
62.50.212 0.226 0.219 0.114
31.250.117 0.111 0.114 0.009
00.106 0.104 0.105 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Monkey IFNG ELISA Kit was designed for the quantitative measurement of Monkey IFNG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

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Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample type: Cell culture supernatant

Sample species: Monkey

Sample dilution: 1:3，1:5

Review: CSB-E10049Mo was used to detect the culture supernatant of cynomollis cells. The OD value of standard curve was 2.780-0.1055, and the OD value of samples was 0.1274-0.0503. The results were lower than the lower limit, which might be due to the dilution factor problem.

By Anonymous

Read more reviews >>

Target Background

Function
(From Uniprot)
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
Gene References into Functions
  1. IFNgamma expression in colonic epithelial cells was regulated by TGFB1. PMID: 25769244
  2. Higher KIR2DL4 copy numbers is associated with an increased IFN-gamma production in NK cell subsets in SIV-infected Mamu-A*01-negative rhesus macaques. PMID: 23449795
  3. Data show that viral set-point in simian immunodeficiency virus diseasewas is associated with expression of interferon gamma -stimulated genes. PMID: 17719201
  4. Low Sociable animals showed alterations in lymph node expression of the immunoregulatory cytokine interferon-gamma. PMID: 18068331
Subcellular Location
Secreted.
Protein Families
Type II (or gamma) interferon family
Tissue Specificity
Released primarily from activated T lymphocytes.
Database Links
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Related pathways
HIF-1 signaling pathway Jak-STAT signaling pathway TGF-beta signaling pathway Necroptosis Antigen processing and presentation IL-17 signaling pathway Natural killer cell mediated cytotoxicity Osteoclast differentiation T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation
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