Product Details

Target Name

chemokine (C-X-C motif) ligand 14

Alternative Names

Cxcl14 ELISA Kit; Bmac ELISA Kit; Kec ELISA Kit; Ks1 ELISA Kit; Mip2g ELISA Kit; Scyb14C-X-C motif chemokine 14 ELISA Kit; B-cell and monocyte-activating chemokine ELISA Kit; Chemokine BRAK ELISA Kit; Kidney-expressed chemokine CXC ELISA Kit; MIP-2G ELISA Kit; Small-inducible cytokine B14 ELISA Kit

Abbreviation

CXCL14

Uniprot No.

Q9WUQ5

Species

Mus musculus (Mouse)

Sample Types

serum, plasma

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	103
	Range %	99-108
1:4	Average %	97
	Range %	92-101
1:8	Average %	89
	Range %	84-93

Recovery

The recovery of mouse CXCL14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	97	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.737	2.652	2.695	2.547
10	2.235	2.276	2.256	2.108
5	1.533	1.604	1.569	1.421
2.5	0.915	0.938	0.927	0.779
1.25	0.557	0.524	0.541	0.393
0.625	0.312	0.327	0.320	0.172
0.312	0.239	0.229	0.234	0.086
0	0.146	0.150	0.148

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse CXCL14 ELISA Kit was designed for the quantitative measurement of Mouse CXCL14 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Background

Function
(From Uniprot)

Chemotactic for CESS B-cells and THP-1 monocytes, but not T-cells.

Gene References into Functions

Platelets are a relevant source of CXCL14. Platelet-derived CXCL14 at the site of vascular lesions might play an important role in vascular repair/regeneration. PMID: 28359053
CXCL14 Tg mice showed a suppressed rate of carcinogenesis, decreased tumour volume, and reduced pulmonary metastasis, as well as an increased survival rate of mice following tumour cell injection. PMID: 25765541
CXCL14 does not seem to play a pivotal role during influenza and Escherichia coli infections of the lung. PMID: 25313607
CXCL14 was able to promote bone metastasis through enhancement of cancer cell tropism to the bone and/or recruitment of bone marrow cells around metastatic cancer cells. PMID: 24534874
In conclusion, our results suggested the important function of Cxcl14 in uNK cells and the proper level of Cxcl14 protein were required to recruit NK cells to pregnant uterus. PMID: 23688424
the transient expression of CXCL14 by Purkinje cells in the developing cerebellum, suggesting that it must be involved in the postnatal maturation of the cerebellum PMID: 22843118
CXCL14 may play an important role in central nervous system regulation of feeding behavior PMID: 20428232
Study identifies CXCL14 as a novel marker of tendon connective tissue. PMID: 21038449
Murine CXCL14 is dispensable for the homeostatic recruitment of antigen-presenting cells toward the periphery and for LC functionality. PMID: 17130243
CXCL14 is a critical chemoattractant of white adipose tissue macrophages and a novel regulator of glucose metabolism that functions mainly in skeletal muscle. PMID: 17724031
These results suggest that CXCL14 plays a causal role in high-fat diet-induced obesity. PMID: 17971304
Data suggest that despite the structural homology and similarity in tissue distribution of human and murine CXCL14, distinct differences point to diverse, species-specific needs for CXCL14 in epithelial immunity. PMID: 18809336
findings demonstrate that early overexpression of PMP22 in a mouse model of Charcot-Marie-Tooth disease type 1A results in a strong up-regulation of CXCL14 which seems to play a novel regulatory role in Schwann cell differentiation PMID: 19111616
CXCL14 is an important paracrine/autocrine modulator regulating trophoblast outgrowth at the maternal-fetal interface during the process of pregnancy establishment. PMID: 19626669
These data indicate the possibility that BRAK expression inhibits tumor cell establishment by regulating interactions between tumor stem cells and NK cells and/or suppressing formation of tumor microvessels. PMID: 19887729

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Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Tissue Specificity

Highly expressed in brain, lung, ovary, muscle and in kidney and liver parenchyma, and at lower levels in bone marrow.

Database Links

KEGG: mmu:57266

STRING: 10090.ENSMUSP00000021970

UniGene: Mm.30211

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Mouse C-X-C motif chemokine 14(CXCL14) ELISA kit

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