Product Details

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.

Target Names

Cxcl14

Uniprot No.

Q9WUQ5

Research Area

Immunology

Alternative Names

Cxcl14; Bmac; Kec; Ks1; Mip2g; Scyb14C-X-C motif chemokine 14; B-cell and monocyte-activating chemokine; Chemokine BRAK; Kidney-expressed chemokine CXC; MIP-2G; Small-inducible cytokine B14

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

23-99aa

Complete Sequence

SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIVTTKS MSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE

Mol. Weight

9.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CXCL14 is a homeostatic chemokine with potent monocyte-recruiting activity, yet its signaling mechanisms remain incompletely characterized, making a reliable recombinant form essential for mechanistic studies. This tag-free recombinant mouse CXCL14 encompasses the full-length mature sequence (residues 23–99) and demonstrates robust chemotactic activity on human monocytes at 1.0–10 ng/ml, providing a suitable basis for chemotaxis and transwell migration assays, monocyte activation studies, and downstream investigation of NF-κB and MAPK signaling pathways. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound innate immune cell readouts, which supports use in sensitive functional assays and in vivo inflammation or tumor biology models in mice. Purity exceeding 95% by SDS-PAGE, combined with the low endotoxin profile, also satisfies the quality thresholds commonly required for ELISA standard curves, antibody validation, and immunogen applications.

Target Background

Function

Chemotactic for CESS B-cells and THP-1 monocytes, but not T-cells.

Gene References into Functions

Platelets are a relevant source of CXCL14. Platelet-derived CXCL14 at the site of vascular lesions might play an important role in vascular repair/regeneration. PMID: 28359053
CXCL14 Tg mice showed a suppressed rate of carcinogenesis, decreased tumour volume, and reduced pulmonary metastasis, as well as an increased survival rate of mice following tumour cell injection. PMID: 25765541
CXCL14 does not seem to play a pivotal role during influenza and Escherichia coli infections of the lung. PMID: 25313607
CXCL14 was able to promote bone metastasis through enhancement of cancer cell tropism to the bone and/or recruitment of bone marrow cells around metastatic cancer cells. PMID: 24534874
In conclusion, our results suggested the important function of Cxcl14 in uNK cells and the proper level of Cxcl14 protein were required to recruit NK cells to pregnant uterus. PMID: 23688424
the transient expression of CXCL14 by Purkinje cells in the developing cerebellum, suggesting that it must be involved in the postnatal maturation of the cerebellum PMID: 22843118
CXCL14 may play an important role in central nervous system regulation of feeding behavior PMID: 20428232
Study identifies CXCL14 as a novel marker of tendon connective tissue. PMID: 21038449
Murine CXCL14 is dispensable for the homeostatic recruitment of antigen-presenting cells toward the periphery and for LC functionality. PMID: 17130243
CXCL14 is a critical chemoattractant of white adipose tissue macrophages and a novel regulator of glucose metabolism that functions mainly in skeletal muscle. PMID: 17724031
These results suggest that CXCL14 plays a causal role in high-fat diet-induced obesity. PMID: 17971304
Data suggest that despite the structural homology and similarity in tissue distribution of human and murine CXCL14, distinct differences point to diverse, species-specific needs for CXCL14 in epithelial immunity. PMID: 18809336
findings demonstrate that early overexpression of PMP22 in a mouse model of Charcot-Marie-Tooth disease type 1A results in a strong up-regulation of CXCL14 which seems to play a novel regulatory role in Schwann cell differentiation PMID: 19111616
CXCL14 is an important paracrine/autocrine modulator regulating trophoblast outgrowth at the maternal-fetal interface during the process of pregnancy establishment. PMID: 19626669
These data indicate the possibility that BRAK expression inhibits tumor cell establishment by regulating interactions between tumor stem cells and NK cells and/or suppressing formation of tumor microvessels. PMID: 19887729

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Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Tissue Specificity

Highly expressed in brain, lung, ovary, muscle and in kidney and liver parenchyma, and at lower levels in bone marrow.

Database Links

KEGG: mmu:57266

STRING: 10090.ENSMUSP00000021970

UniGene: Mm.30211

Code	CSB-AP001181MO
Abbreviation	Recombinant Mouse Cxcl14 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Mouse C-X-C motif chemokine 14 protein (Cxcl14) (Active)

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