Mouse Complement fragment 5a,C5a ELISA Kit

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Mouse Complement fragment 5a,C5a ELISA Kit

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Citations (4) Protocol MSDS

Code	CSB-E08514m
Size	96T,5×96T,10×96T
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Product Details
Citations (4)
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Target Name

Complement fragment 5a,C5a

Abbreviation

C5a

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

39 pg/mL-2500 pg/mL

Sensitivity

9.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:20	Average %	85
	Range %	81-90
1:40	Average %	98
	Range %	91-105
1:80	Average %	89
	Range %	84-94
1:160	Average %	90
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2500

2.844

2.734

2.789

2.662

1250

2.331

2.197

2.264

2.137

625

1.488

1.528

1.508

1.381

312.5

0.810

0.829

0.819

0.693

156.25

0.546

0.521

0.533

0.407

0.357

0.363

0.360

0.234

0.221

0.214

0.218

0.091

0.128

0.125

0.127

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse C5a ELISA Kit was designed for the quantitative measurement of Mouse C5a protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL.

The activation of complement C5a-C5aR1 axis in astrocytes facilitates the neuropathogenesis due to EV-A71 infection by upregulating CXCL1 P Zhu, W Ji, D Li, F Wang, T Sun, H Yang,Journal of Virology,2024
Blockade of C5aR1 alleviates liver inflammation and fibrosis in a mouse model of NASH by regulating TLR4 signaling and macrophage polarization K Jiang,Journal of gastroenterology,2023
Cryogel/hydrogel biomaterials and acupuncture combined to promote diabetic skin wound healing through immunomodulation TY Chen,Biomaterials,2020
Heparin-like surface modification of polyethersulfone membrane and its biocompatibility Min Tang et al,Journal of Colloid and Interface Science,2012

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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