Human Complement fragment 5a,C5a ELISA Kit

Code CSB-E08512h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Alternative Names
N/A
Abbreviation
C5a
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
39 pg/ml-2500 pg/ml
Sensitivity
9.75 pg/ml
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
 
Linearity
 
Recovery
 
Typical Data
 
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The human C5a ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure human C5a in serum, plasma, or tissue homogenates. It is based on the Sandwich-ELISA mechanism. C5a in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated C5a antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the C5a bound in the initial step. The C5a concentration can be calculated according to the standard curve. This kit is tested with high sensitivity and strong specificity.

C5a is a pro-inflammatory polypeptide that is generated when the complement cascade of serum proteins is activated in response to external surfaces like bacteria and tissue that has been injured by physical or chemical harm. C5a has potent biological actions that result in inflammatory consequences. C5a is a strong chemoattractant that plays a role in the recruitment of inflammatory cells like neutrophils, eosinophils, monocytes, and T lymphocytes, as well as the activation of phagocytic cells, the release of granule-based enzymes, and the generation of oxidants, all of which can contribute to innate immune functions or tissue damage. Increasing evidence suggests that C5a serves as a crucial link between innate and adaptive immune processes, potentially expanding C5a's role in inflammation. Overexpression or under-regulation of C5a is implicated in human and/or experimental models of inflammatory conditions, such as rheumatoid arthritis (RA), and osteoarthritis, and inflammatory bowel diseases.

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