Pig complement fragment 5a (C5a) ELISA kit

Code CSB-EQ027637PI
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Pig C5a ELISA Kit was designed for the quantitative measurement of Pig C5a protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.375 ng/mL-600 ng/mL and the sensitivity is 2.34 ng/mL .

Target Name complement fragment 5a (C5a)
Abbreviation C5a
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 9.375 ng/mL-600 ng/mL
Sensitivity 2.34 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of pig C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %99
Range %91-109
1:2Average %87
Range %82-93
1:4Average %102
Range %98-106
1:8Average %98
Range %92-104
The recovery of pig C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)9692-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
6002.385 2.284 2.335 2.160
3001.903 1.802 1.853 1.678
1501.376 1.355 1.366 1.191
750.903 0.914 0.909 0.734
37.50.650 0.661 0.656 0.481
18.750.411 0.422 0.417 0.242
9.3750.317 0.312 0.315 0.140
00.175 0.174 0.175  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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