Rat Complement fragment 5a,C5a ELISA Kit

Code CSB-E08513r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Rat C5a ELISA Kit was designed for the quantitative measurement of Rat C5a protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

Target Name Complement fragment 5a,C5a
Alternative Names
Abbreviation C5a
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 102  
Range % 96-108  
1:2000 Average % 94  
Range % 91-98  
1:4000 Average % 96  
Range % 88-99  
1:8000 Average % 92  
Range % 86-98  
The recovery of rat C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 92-104  
EDTA plasma (n=4) 94 90-98  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.241 2.140 2.191 2.053  
50 1.804 1.703 1.754 1.616  
25 1.215 1.204 1.210 1.072  
12.5 0.713 0.702 0.708 0.570  
6.25 0.464 0.475 0.470 0.332  
3.12 0.328 0.339 0.334 0.196  
1.56 0.245 0.251 0.248 0.110  
0 0.137 0.138 0.138    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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