Rat Complement fragment 5a,C5a ELISA Kit

Rat Complement fragment 5a,C5a ELISA Kit

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Rat Complement fragment 5a,C5a ELISA Kit

Protocol

Code	CSB-E08513r
Size	96T,5×96T,10×96T
Trial Size
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Description

This Rat C5a ELISA Kit was designed for the quantitative measurement of Rat C5a protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

Target Name

Complement fragment 5a,C5a

Alternative Names

N/A

Abbreviation

C5a

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

1.56 ng/mL-100 ng/mL

Sensitivity

0.39 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	102
	Range %	96-108
1:2000	Average %	94
	Range %	91-98
1:4000	Average %	96
	Range %	88-99
1:8000	Average %	92
	Range %	86-98

Recovery

The recovery of rat C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-104
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.241	2.140	2.191	2.053
50	1.804	1.703	1.754	1.616
25	1.215	1.204	1.210	1.072
12.5	0.713	0.702	0.708	0.570
6.25	0.464	0.475	0.470	0.332
3.12	0.328	0.339	0.334	0.196
1.56	0.245	0.251	0.248	0.110
0	0.137	0.138	0.138

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Check Code：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.