Bovine complement fragment 5a (C5a) ELISA kit

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Bovine complement fragment 5a (C5a) ELISA kit

Specification

Code

CSB-EQ027637BO

See More Details

Product Type

ELISA kit

Size

96T,5×96T,10×96T

Lead Time

3-5 working days

Abbreviation

C5a

Target Name

complement fragment 5a (C5a)

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

4.69 ng/mL-300 ng/mL

Sensitivity

1.17 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-93
1:2	Average %	101
	Range %	97-104
1:4	Average %	94
	Range %	88-99
1:8	Average %	93
	Range %	89-97

Recovery

The recovery of bovine C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	90	86-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
300	2.698	2.489	2.594	2.487
150	2.132	2.245	2.189	2.082
75	1.576	1.706	1.641	1.534
37.5	1.133	1.050	1.092	0.985
18.75	0.714	0.677	0.696	0.589
9.38	0.440	0.466	0.453	0.346
4.69	0.278	0.256	0.267	0.160
0	0.106	0.107	0.107

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C5a ELISA Kit

C5a ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-E08512h	Human Complement fragment 5a,C5a ELISA Kit	serum, plasma, tissue homogenates	0.156 ng/mL

C5a ELISA Kit for Oryctolagus cuniculus (Rabbit)

Code	Product Name	Sample Types	Sensitivity
CSB-E13247Rb	Rabbit complement fragment 5a,C5a ELISA Kit	serum, plasma, tissue homogenates	0.156 ng/mL

C5a ELISA Kit for Rattus norvegicus (Rat)

Code	Product Name	Sample Types	Sensitivity
CSB-E08513r	Rat Complement fragment 5a,C5a ELISA Kit	serum, plasma, cell culture supernates, tissue homogenates	0.39 ng/mL

C5a ELISA Kit for Canis lupus familiaris (Dog) (Canis familiaris)

Code	Product Name	Sample Types	Sensitivity
CSB-EQ027637DO	Dog complement fragment 5a (C5a) ELISA kit	serum, plasma, tissue homogenates	1.95 ng/mL

C5a ELISA Kit for Sus scrofa (Pig)

Code	Product Name	Sample Types	Sensitivity
CSB-EQ027637PI	Pig complement fragment 5a (C5a) ELISA kit	serum, plasma, tissue homogenates	2.34 ng/mL

C5a ELISA Kit for Mus musculus (Mouse)

Code	Product Name	Sample Types	Sensitivity
CSB-E08514m	Mouse Complement fragment 5a,C5a ELISA Kit	serum, plasma, tissue homogenates	9.75 pg/mL

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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