Bovine complement fragment 5a (C5a) ELISA kit

Code CSB-EQ027637BO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
complement fragment 5a (C5a)
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
4.69 ng/mL-300 ng/mL
1.17 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of bovine C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %90
Range %88-93
1:2Average %101
Range %97-104
1:4Average %94
Range %88-99
1:8Average %93
Range %89-97
The recovery of bovine C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-103
EDTA plasma (n=4)9086-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
3002.698 2.489 2.594 2.487
1502.132 2.245 2.189 2.082
751.576 1.706 1.641 1.534
37.51.133 1.050 1.092 0.985
18.750.714 0.677 0.696 0.589
9.380.440 0.466 0.453 0.346
4.690.278 0.256 0.267 0.160
00.106 0.107 0.107
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Bovine C5a ELISA Kit was designed for the quantitative measurement of Bovine C5a protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.69 ng/mL-300 ng/mL and the sensitivity is 1.17 ng/mL.

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