Bovine complement fragment 5a (C5a) ELISA kit

Code CSB-EQ027637BO
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Product Type ELISA kit
Size 96T,5×96T,10×96T
Abbreviation C5a
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 4.69 ng/mL-300 ng/mL
Sensitivity 1.17 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Availability 3-5 working days
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %88-93
1:2Average %101
Range %97-104
1:4Average %94
Range %88-99
1:8Average %93
Range %89-97
Recovery
The recovery of bovine C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-103
EDTA plasma (n=4)9086-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
3002.698 2.489 2.594 2.487
1502.132 2.245 2.189 2.082
751.576 1.706 1.641 1.534
37.51.133 1.050 1.092 0.985
18.750.714 0.677 0.696 0.589
9.380.440 0.466 0.453 0.346
4.690.278 0.256 0.267 0.160
00.106 0.107 0.107
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