Name: Mouse EGF-containing fibulin-like extracellular matrix protein 1(EFEMP1) ELISA kit
Brand: CUSABIO
SKU: CSB-EL007450MO

Product Details

Target Name

EGF-containing fibulin-like extracellular matrix protein 1

Alternative Names

Efemp1 ELISA Kit; Fbln3EGF-containing fibulin-like extracellular matrix protein 1 ELISA Kit; Fibulin-3 ELISA Kit; FIBL-3 ELISA Kit

Abbreviation

EFEMP1

Uniprot No.

Q8BPB5

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse EFEMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-100
1:2	Average %	88
	Range %	84-92
1:4	Average %	92
	Range %	88-96
1:8	Average %	104
	Range %	100-108

Recovery

The recovery of mouse EFEMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	97	93-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.243	2.168	2.206	2.033
1000	1.553	1.605	1.579	1.406
500	1.016	1.013	1.015	0.842
250	0.597	0.574	0.586	0.413
125	0.388	0.412	0.400	0.227
62.5	0.324	0.337	0.331	0.158
31.25	0.277	0.271	0.274	0.101
0	0.177	0.169	0.173

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse EFEMP1 ELISA Kit was designed for the quantitative measurement of Mouse EFEMP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Customer Reviews and Q&A

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■ Q&A

Regarding your ELISA kit CSB-EL007450MO, a customer is about to use it and they harvested their cells (for cell lysate samples) in RIPA buffer rather than PBS. Do you know if RIPA buffer would cause any negative effects with this kit, or is RIPA going to be expected to be OK?

Thanks for your inquiry!
Generally speaking, RIPA will contain SDS, Triton X-100, NP-40 and other components. Customers now need to confirm the RIPA buffer formula. Pls note that it can not contain SDS (it will destroy the hydrogen bonds of the protein, which will lead to protein denaturation.);
For Triton X-100, NP-40, you need to control the low concentration range (generally not more than 1%, but it is not verified); For the entire RIPA buffer, sorry we have not tried this preparation method before, so we can only suggest you do a pretest if you want to try it.
Pls let me know if you have any further questions. Thank you.

Can you please advise on the immunogens used to raise the antibodies from this kit? Were they full-length or partial-length? And were the antibodies polyclonal or monoclonal?

Thanks for your inquiry!CSB-EL007450MO
Capture antibody is rat monoclonal antibody and immunogen is NS0-derived recombinant protein,full-length.
Detection antibody is goat polyclonal antibody and immunogen is
NS0-derived recombinant protein,partial.

Target Background

Function
(From Uniprot)

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.

Gene References into Functions

TWEAK promotes migration and invasion in murine embryonic fibroblasts through a mechanism dependent on ERKs activation and Fibulin 3 down-regulation. PMID: 28383766
Mutant fibulin-3 causes proteoglycan accumulation, reduction of MMP-2 and MMP-9, but increase of TIMP-3, and impairs diffusion across Bruch's membrane. PMID: 28622396
Define the Efemp1-p27(Kip1) axis as a potential marker module of PDAC cell sensitivity towards dual PI3K-mTOR inhibitors. PMID: 23470560
The Efemp1 knock-in mice reconstitute the most important histopathologic symptoms of both Malattia leventinese and age-related macular degeneration. PMID: 17664227
fibulin-3 plays an important role in maintaining the integrity of fascia connective tissues and regulates aging. PMID: 17872905
both fibulin-3 and -5 are important in maintaining pelvic organ support in mice. PMID: 19095964
Data suggest a role for fibulin-3 in tissue maintenance and regeneration in the adult olfactory pathway. PMID: 19520159

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Subcellular Location

Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.

Protein Families

Fibulin family

Database Links

KEGG: mmu:216616

STRING: 10090.ENSMUSP00000020759

UniGene: Mm.44176

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Code	CSB-EL007450MO
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Mouse EGF-containing fibulin-like extracellular matrix protein 1(EFEMP1) ELISA kit

Product Details

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Target Background