Recombinant Mouse EGF-containing fibulin-like extracellular matrix protein 1 (Efemp1)

Code CSB-EP007450MO
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP007450MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007450MO
MSDS
Size Pls inquire
Source Baculovirus
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Efemp1
Uniprot No.
Alternative Names
Efemp1; Fbln3EGF-containing fibulin-like extracellular matrix protein 1; Fibulin-3; FIBL-3
Species
Mus musculus (Mouse)
Expression Region
18-493
Target Protein Sequence
QYT EETITYTQCT DGYEWDPIRQ QCKDIDECDI VPDACKGGMK CVNHYGGYLC LPKTAQIIVN NEHPQQETPA AEASSGATTG TVAARSMATS GVVPGGGFMA SATAVAGPEV QTGRNNFVIR RNPADPQRIP SNPSHRIQCA AGYEQSEHNV CQDIDECTSG THNCRTDQVC INLRGSFTCQ CLPGYQKRGE QCVDIDECTV PPYCHQRCVN TPGSFYCQCS PGFQLAANNY TCVDINECDA SNQCAQQCYN ILGSFICQCN QGYELSSDRL NCEDIDECRT SSYLCQYQCV NEPGKFSCMC PQGYEVVRSR TCQDINECET TNECREDEMC WNYHGGFRCY PRNPCQDHYV LTSENRCVCP VSNTMCRELP QSIVYKYMSI RSDRSVPSDI FQIQATMIYA NTINTFRIKS GNENGEFYLR QTSPVSAMLV LVKSLSGPRE YIVDLEMLTV SSIGTFRTSS VLRLTIIVGP FSF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
Gene References into Functions
  1. TWEAK promotes migration and invasion in murine embryonic fibroblasts through a mechanism dependent on ERKs activation and Fibulin 3 down-regulation. PMID: 28383766
  2. Mutant fibulin-3 causes proteoglycan accumulation, reduction of MMP-2 and MMP-9, but increase of TIMP-3, and impairs diffusion across Bruch's membrane. PMID: 28622396
  3. Define the Efemp1-p27(Kip1) axis as a potential marker module of PDAC cell sensitivity towards dual PI3K-mTOR inhibitors. PMID: 23470560
  4. The Efemp1 knock-in mice reconstitute the most important histopathologic symptoms of both Malattia leventinese and age-related macular degeneration. PMID: 17664227
  5. fibulin-3 plays an important role in maintaining the integrity of fascia connective tissues and regulates aging. PMID: 17872905
  6. both fibulin-3 and -5 are important in maintaining pelvic organ support in mice. PMID: 19095964
  7. Data suggest a role for fibulin-3 in tissue maintenance and regeneration in the adult olfactory pathway. PMID: 19520159

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Subcellular Location
Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
Protein Families
Fibulin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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