Mouse Gastrin-releasing peptide(GRP) ELISA kit

Instructions
Code CSB-EL009940MO
Size 96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name gastrin-releasing peptide
Alternative Names Grp ELISA Kit; Gastrin-releasing peptide ELISA Kit; GRP) [Cleaved into: Neuromedin-C ELISA Kit; GRP-10)] ELISA Kit
Abbreviation GRP
Uniprot No. Q8R1I2
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 39 pg/mL-2500 pg/mL
Sensitivity 9.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %98
Range %92-102
1:2Average %98
Range %91-106
1:4Average %95
Range %89-98
1:8Average %96
Range %92-104
Recovery
The recovery of mouse GRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)8982-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
25002.738 2.616 2.677 2.512
12502.158 2.010 2.084 1.919
6251.390 1.368 1.379 1.214
312.50.866 0.886 0.876 0.711
156.250.560 0.542 0.551 0.386
78.1250.351 0.363 0.357 0.192
39.0630.256 0.250 0.253 0.088
00.162 0.167 0.165  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Stimulates the release of gastrin and other gastrointestinal hormones (By similarity). Contributes to the perception of prurient stimuli and to the transmission of itch signals in the spinal cord that promote scratching behavior
Gene References into Functions
  1. These data suggest that GRPR expression in fibroblast-like synoviocytes (FLS)and that exogenous GRP are able to activate FLS invasion. This effect occurs at least in part through the AKT activation. Therefore, understanding of the GRP/GRPR pathway could be relevant in the development of FLS-targeted therapy for Rheumatoid arthritis . PMID: 28733141
  2. Fos and phosphorylation of extracellular signal-regulated kinases (ERK) were used to look for evidence that interneurons expressing GRP were activated following intradermal injection of chloroquine. PMID: 27270268
  3. GrpmRNA and protein are upregulated in dorsal root ganglias, but not in the spinal cord, of mice with chronic itch. PMID: 27068287
  4. GRP is expressed by a distinct population of excitatory interneurons in laminae I-II that are likely to be involved in the itch pathway PMID: 25496164
  5. BNP-NPRA signaling is involved in both itch and pain and does not function upstream of the GRP-GRPR dedicated neuronal pathway. PMID: 24438367
  6. nonamidated peptides derived from the C terminus of pro-GRP are expressed in significant quantities in colorectal cancer cell lines PMID: 22202166
  7. GRP delays the phase of the clock during the early night by prolonging day-like membrane properties of SCN cells PMID: 21454290
  8. The GRP receptor system does not mediate kappa opioid receptor antagonist (GNTI)-induced scratching. The kappa opioid system is involved, at least in part, in the scratch suppressing activity of the kappa opioid receptor agonist nalfurafine. PMID: 21126550
  9. These studies demonstrate that (64)Cu-SarAr-SA-Aoc-bombesin(7-14) and (64)Cu-SarAr-SA-Aoc-GSG-bombesin(7-14) bound with high affinity to GRPR-expressing cells. PMID: 21321264
  10. A general possible effect of GRP on cortical inhibitory transmission, was shown. PMID: 20836873
  11. GRP/GRP-R play a transient and non-critical role in intestinal development PMID: 11960700
  12. GRP has a role in regulation of fear and memory for fear in the mouse amygdala PMID: 12526815
  13. Potential new role for gastrin-releasing peptide -BB2 signaling within the circadian clock. PMID: 16319315
  14. New evidence for the occurrence of marked effects of bombesin/gastrin-releasing peptide concerning both the articular chondrocytes and the inflammatory process is obtained in this study PMID: 17911468
  15. GRP nerve fibers were markedly high in the epidermis of atopic dermatitis mice PMID: 19571818

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Subcellular Location Secreted, Cytoplasmic vesicle, secretory vesicle lumen
Protein Families Bombesin/neuromedin-B/ranatensin family
Tissue Specificity Detected in the suprachiasmatic nucleus in the hypothalamus (PubMed:28280205). Detected in peptidergic dorsal root ganglion neurons (at protein level) (PubMed:17653196). Highly expressed both in the lateral nucleus of the amygdala, and in regions sending
Database Links

KEGG: mmu:225642

STRING: 10090.ENSMUSP00000025395

UniGene: Mm.20298

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